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1、中国农学通报2014,30(33):156-161ChineseAgriculturalScienceBulletin再生丝素膜的生物安全性评估试验周婵，王介平，吕金凤，王海燕，曾秀（重庆市畜牧科学院，重庆402460）摘要：以再生丝素蛋白为原料，采用旋转干燥法，通过控制温度和湿度及乙醇改性，最终得到不溶于水的丝素膜。为了评价再生丝素膜的生物安全性，进行体外细胞毒性、全身过敏反应、迟发型超敏反应、急性毒性反应、热原反应以及溶血性试验。结果：用丝素膜浸提液对体外培养的成纤维细胞进行处理后，细胞平均相
2、对增殖率为92.3%，属于细胞毒性I级。小鼠经由100%丝素膜浸提液多次致敏后再次激发，未产生全身性过敏反应；供试小鼠静脉大剂量注射100%丝素膜浸提液后全部存活，无急性全身毒性反应，7天观察期内小鼠的体质量逐步增长；用100%丝素膜浸提液对家兔进行静脉注射后，兔的单只体温升高幅度为0.2~0.5℃，未超0.6℃上限，总和也未超1.4℃上限；选取不同剂量（100%、50%、25%、10%）丝素膜浸提液对家兔进行溶血试验，溶血率为4.02%、2.57%、1.56%、1.01%，均小于5%。综上所述，
3、研究制备的再生丝素膜状材料，具有良好的生物安全性。关键词：再生丝素；蛋白；膜状材料；生物安全性中图分类号：S881.3；R318.08文献标志码：B论文编号：2014-2155EvaluationoftheBiologicalSafetyofSFMembraneZhouChan,WangJieping,LVJinfeng,WangHaiyang,ZengXiu(ChongqingAcademyofAnimalSciences,Chongqing402460)Abstract:Water-fasts
4、ilkfibroinfilmswerecreatedfromregeneratedsilkfibroinbydryingatacontrolledtemperatureandhumidityanddegeneratingwithethanol.Toevaluatethebiosafetyofthesesilkfibroinmembranes,cytotoxicitytestinvitro,generalallergic,delayed-typehypersensitivityassay,acute
5、toxicity,pyrogenicreactionandhemolytictestonerythrocytesofrabbitswereconducted.Theresultsshowedthat:afterinvitroculturedfibroblastsweretreatedwithsilkfibroinmembraneleachingliquor,theyproliferatedappropriatelyat92.3%,indicatingthecytotoxicityofsilkfib
6、roinmembraneleachingliquorwasgradeI;afterrepeatedinjectionsof100%silkfibroinmembraneleachingliquor,nomicepresentedgeneralizedanaphylaxis;allthemicethatreceivedinjectionsof100%silkfibroinmembraneleachingliquorsurvived,noacutegeneraltoxicreactions;thebo
7、dyweightofmicegraduallyincreasedwithin7days.Afterinjectionof100%silkfibroinmembraneleachingliquorintotheearveinofJapanesebigearedrabbits,thebodytemperatureofperrabbitincreasedby0.2-0.5℃,butdidnotexceedtheupperlimitof0.6,and1.4℃intotal.Thehemolysistest
8、showedthatthehemolyticratesof100%,50%,25%,and10%silkfibroinmembraneleachingliquorwere4.02%,2.57%,1.56%,and1.01%，respectively,allrateswerelowerthan5%.Inshort,thedataindicatethatregeneratedsilkfibroinfilmsexhibitgoodbiosafety.Keywords:regenerate
