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1、中国组织工程研究与临床康复第14卷第6期2010–02–05出版JournalofClinicalRehabilitativeTissueEngineeringResearchFebruary5,2010Vol.14,No.6兔骨髓间充质干细胞体外分离培养及多向诱导分化*★徐成峰，胡大海，赵周婷，张万福，白晓智，蔡维霞Isolation,cultureandmultipledifferentiationsofrabbitbonemarrow-derivedmesenchymalstemcellsinvitroXuCheng-feng,HuDa-hai,ZhaoZhou-ting
2、,ZhangWan-fu,BaiXiao-zhi,CaiWei-xiaAbstractDepartmentofBurnsandCutaneousBACKGROUND:Mesenchymalstemcells(MSCs),withlowimmunogenicity,canregulatecellularimmunityandmitigategraftSurgery,Burnsrejection,whichhasagoodprospectintissueengineering.However,itisrarelypresentinbonemarrow.CenterofPLA,Xij
3、ingOBJECTIVE:Toexploreanisolationandculturemethodoftherabbitbonemarrow-derivedMSCs,toobservethebiologicalHospital,theFourthMilitaryMedicalcharacteristicsanddifferentiationpotentialofbonemarrow-derivedMSCs.UniversityofChineseMETHODS:MSCswereisolatedfromrabbittibiabonemarrowbycombinationofgrad
4、ientcentrifugationanddifferentPLA,Xi’an710032,adherentmethod,thenproliferationinvitro.Morphologywasexaminedbyphasecontrastmicroscopy,andthegrowthcurveShaanxiProvince,ofculturedMSCswasdrawnviaMTTresults.MSCsweretreatedwithosteogeneticinductor(L-DMEM/F12,10%fetalbovineChinaserum,0.1μmol/Ldexam
5、ethasone,200μmol/LvitaminC,10mmol/Lβ-phosphoglycerol),adiposeinductor(L-DMEM/F12,10%XuCheng-feng★,FBS,1μmol/Ldexamethasone,200μmol/Lantifani,0.5mmol/LIBMX,10μg/mLinsulin),andchondrocytesinductorStudyingformaster’s(L-DMEM/F12,10%FBS,10μg/LTGF-β1,0.1μmol/Ldexamethasone,50μmol/LvitaminC,6.25mg/
6、Linsulin)todifferentiateddegree,Physician,intoosteoblast,dipocytesandchondrocytes.Andthedifferentiatedcellswereidentifiedbyalkalinephosphatasestaining,oilredDepartmentofBurnsandCutaneousOstaining,andtoluidinebluestaining,respectively.Surgery,BurnsRESULTSANDCONCLUSION:Bonemarrow-derivedMSCsca
7、nbeisolatedandculturedbythecombinationofgradientCenterofPLA,Xijingcentrifugationanddifferentadherentmethodinvitro,whichhavethebetterpotentialityofproliferationandmulti-directionalHospital,theFourthdifferentiation.Mostlyoftheprimaryandpassagedcellsw
