资源描述：
《玉米花粉诱导小黑麦单倍体技术建立与单倍体诱导率遗传规律分析》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、关键词：小黑麦；玉米花粉；单倍体；遗传规律论文类型：B（应用研究）IIAbstractHaploidbreedingisapowerfultooltoenhancebreedingprocessandimprovebreedingefficiency.Andantherorpollencultureismainlyappliedintraditionalhaploidbreeding.However,thecomplexprocessandhigherfrequencyofcolonelvariationmakeantherorpollencu
2、lturequitedifficultinlargescalesapplication.Itissimpleandfeasibletoobtainwheathaploidinducedbymaizepollen.Thepresentstudyisaimedtoestablishthetechnologyoftriticalehaploidinducedbymaizepollen,andunderstandgeneticofhaploidinductionrate.Thisstudywillprovidesometheoreticalbasis’
3、sforanefficienttriticalebreedingtechnology.Somewinterandspringtriticalegenotypeswereinducedbyfourmethods,A:maizepollenandchemicals(inducedbymaizepollenandsprayedwithDMSO40ml/L,COL4g/L,Inositol100mg/L,IAA20mg/Land2,4-D20mg/L）;B:chemicals（DMSO40ml/L,COL4g/L,Inositol100mg/L,IAA
4、20mg/Land2,4-D20mg/L）;C:maizepollenandhormone(inducedbymaizepollen,thensprayedwith100mg/L2,4-Dand30ml/LDMSO);D:hormoneonly(100mg/L2,4-Dand30ml/LDMSO).Theeffectsofhaploidembryosrescuingmedium,maizegenotypeandtriticalegenotypeonhaploidembryoinductionwerealsostudiedtoestablisht
5、hetechnologyofinducingtriticalehaploidbymaizepollen.Throughthecomplete6×6diallelcrossingbetween6triticalevarietieswithdifferenthaploidembryofrequency,thecombinationability,geneticeffect,heterosisofhaploidembryofrequenciesofparent’sheritabilityandgeneticcorrelationofF1offspri
6、ngwereanalyzed.Themainresultswereasfollows:1.Inductionbymaizepollenandhormoneisthebestmeansamongthefourmethodstoinducetriticalehaploidembryofor6.65%haploidembryos,whichishigherthantheother3methodsinthisexperiment.2.Haploidembryocouldbeinducedbymaizepollenandchemicalsinmosttr
7、iticalegenotypes.However,thetriticalehaploidinductionratesvariedamongdifferentgenotypes.Thetriticalegenotype07H261-62/NTH2146-2isthebestonewithhigherinductionratehaploidembryo.Theinductionratesofhaploidembryodifferentiatesignificantlyamongdifferenttypesofmaizetoo,andwaxycorn
8、isthebestoneamongthethreemaizetypestestedintheexperiment.3.Thereisnosignifi
