SZDBZ132-2015-小反刍兽疫抗体检测竞争酶联免疫吸附法.pdf

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1、SZDB/Z132-2015ICS11.220B41SZDB/Z深圳市标准化指导性技术文件SZDB/Z132-2015小反刍兽疫抗体检测竞争酶联免疫吸附法DetectionMethodofCompetitiveenzyme-linkedimmunosorbentassayforPestedespetitsruminants2015-02-27发布2015-03-01实施发布深圳市市场监督管理局1SZDB/Z132-2015目次前言..............................................................

2、................................................................................................II1范围................................................................................................................................................................12规范性引用文件.........

3、...................................................................................................................................13原理................................................................................................................................................

4、................14缩略语............................................................................................................................................................15试剂和材料...............................................................................................

5、.....................................................26主要器械和设备............................................................................................................................................27PPRV重组N蛋白抗原的制备.............................................................

6、.......................................................38PPRV单克隆抗体的制备.............................................................................................................................49待测样品的采集和处理.........................................................................

7、.......................................................510竞争酶联免疫吸附（C-ELISA）试验.......................................................................................................6附录A...............................................................................................

8、...............................................