nagase作为甲壳类动物丰度指示因子的可行性分析

《nagase作为甲壳类动物丰度指示因子的可行性分析》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、万方数据浙江人学硕士学位论文ABSTRACTChitobiase科·Acetyl-13一D—glucosaminidase，NAGase)isfoundinvolvedinexoskeletondegradationduringthemoultingprocessofarthropods．Inaquaticspecies，thenx)ultingfluidisreleasedintotheaqueottsenvironment,andthepresentactivityoftheNAGasether

2、eincanbeusedtofollowthepopulationsInthisstudy,threespeciesofChdocera，ie．DaphnmmagnaStraus，Moinasp．，andSimocephalussp．wereusedastestorganismstostudydynamicpropertiesofthefree-livingNAGase．Theenzymereleasedbyallofthespeciesren：】ainedstableattemperature％3

3、4"Candtheactivityremainedhigh(110lessthan75％ofthemaximal)inpHof6．5—9．0．TheenzymereleasedbytheDmagnashoweditsKmofO．072mmoI／LandVmof1．281amol／L／htowardsthesubstrateof4-methylumbelliferylN—acetyl-[3-D—glncosaminide(MtW—NAG)．Theenzymereleasedbythesespecies

4、wassimilar,withrespecttoKmaswellaspHandtemperatm-estability,tothosespemmtedfromcrustaceans，buthadcertaindifferencefromthoseresideinmicroorganismsandplants．Momasp．andSimocephalussp．wereusedastestorganismstostudytheimpactofetoxazoleonthechangeofamouI】tof

5、NAGasereleasedinwatercircumstance(measuredbyenzymeactivity)andproductionofneonates(measuredbynumberofneonatesproducedperfemale)．ThelinearcorrelativeequationwasestablishedbytakingtheNAGaseactivityasthedependentvariable(y)andthenumberofneonatesproducedpe

6、rferruleastheindependentvariable(x)．ForMoma印．，thelinearcorrelationcoefficientis0．2andthePvalueis0．4，forSimocephalussp．，thelinearcorrelationcoefficientisO．7andthePvalueis0．08．TheweakcorrelationfortheMomasp．wasmainlyduetotheletthatthespecieshadaveryshort

7、life．ThenaturalmortalityoftheF0reached50％incontrolgroupatendofthe14一dayexperiment．ThissuggeststhatthesurvivalofF0iscriticalforrmintingasoundlinearrelationshipbetweenthequantityofNAGaseandreproductionrateofthespecies万方数据浙江大学硕士学位论文Theimpactofthreetypesof

8、insecticide，i．e．1ufenuron,diafenthiuron,andetoxazole，Oilpopulationdevelopmentandtherelatingchan伊inamountoftheNAGasetobereleasedwasstudiedusingDaplm&magnaStraus，Moinasp．，andSimocephalussp．astestspecies．AmountofNAGasereleased(countedwitha