蛋白激酶c-β2f衔接蛋白氧化应激通路介导早产儿氧暴露后体内活性氧簇产生的-研究

《蛋白激酶c-β2f衔接蛋白氧化应激通路介导早产儿氧暴露后体内活性氧簇产生的-研究》由会员上传分享，免费在线阅读，更多相关内容在应用文档-天天文库。

1、万方数据能介导早产儿氧暴露后体内ROS的产生。产关键词：蛋白激酶C—p／衔接蛋白：高体积分数氧：活性氧簇：婴儿，早万方数据ProteinkinaseC-beta／the66一kDaisoformofthegrowthfactoradaptorShcoxidativestresspathwayinmediatingtheproductionofthereactiveoxygenspeciesinhyperoxia-exposedprematureinfantAbstractobjectiveTostud

2、ythepossiblemitochondriaoxidativestresssignaltransductionpathway，whichprobablymediatedtheproductionofreactiveoxygenspeciesinhyperoxia—exposedprematureinfants．MethodsControlgroup：twentycasesofprematureinfantswhosegestationalagewasbelow34weekswerediagnose

3、dasnorespiratorydistresssyndromeandrespiratoryfailure[exposedtoairover48h,pulseoxygensaturation(Sp02)>85％)]inDepartmentofNeonatology,theAffiliatedHospitalofSiChuanMedicalUniversity，fromJuly2012toDecember2012．Thecontrolgroup’Smainreasonforinhospitalwaspr

4、ematureinfants．Hyperoxiagroup：twentycasesofprematureinfantswiththesamegestationalagewerediagnosedasrespiratoryfailure(treatedwithexposuretoover400mL／Loxygenwithin48h一69hinthesameroomsimultaneously)．Therespiratoryfailuresincludetwotypes：Itypefailure：arte

5、rialbloodoxygenpartialpressure(Pa02)<6．67kPa；IItypefailure：Pa02<6．67kPa，arterialbloodc02partialpressure(PaC02)>6．67kPa．Themainreasonofhospitalizationwaswithvaryingdegreesofrespiratorydistresssyndromewithrespiratoryfailureinhyperoxiagroup．Twogroupsofinfa

6、ntsweretakento．4．万方数据hospitalforO．25～10．00hafterbirth．Andwiththefollowingdiseasewereexcluded：(1)ABOhemolysisandglucose-6一phosphatedehydrogenase(G6PD)deficient；(2)Congenitalheartdisease；(3)Convulsions，intracranialandgastrointestinalbleeding；(4)Infection．

7、AfterauthorizedbytheethicscommitteeoftheAffiliatedHospitalofLuZhouMedicalCollegeandfamiliesafterinformedconsent，twogroupsofpreterminfantsweresampledblood2mlwhichwasputtedinheparinanticoagulanttub72hafteradmissionfromradialarterybloodaftersterilization．B

8、lendfreshdilutedanticoagulant2mlwithHank'sliquidAccordingtotheproportionof1：1，absorbthefreshdilutedblood4mlthroughastraw,addtoonthesurfaceofthelymphocyteseparationliquidalongthecentrifugaltubewallslowly,horizontalcentrifugal，extr