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1、渝东北山区野葛中葛粉和总黄酮含量动态变化探究[摘要]目的通过对渝东北山区野葛中葛粉和总黄酮含量测定,总结其成分在1年中随季节的变化规律,从而确定每年最佳采收时间。方法2011年6月〜2012年5月每月定期采集渝东北山区产野葛样本,切成2〜3mm薄片,自然干燥。通过文献调研,方法稳定性考察确定采用组织捣碎水沉淀法测定野葛葛粉含量;采用UV紫外分光光度法,以葛根素为对照品,在波长250nm处测定样品溶液吸收度,计算出野葛总黄酮含量,从而得到渝东北山区产野葛在1年里葛粉和总黄酮含量随季节的变化规律。结果经实验测定,渝东北山区野葛葛粉和总黄酮含量全年变化趋势基本一致,变化规律为葛粉含量
2、在8.9%〜25.3%之间,从11月〜次年2月最高,3、8、9、10月其次,4〜7月最低;总黄酮含量在2.13%〜11.6%之间,11月〜次年3月高于9%,8〜10月约6%,4〜7月较低在2%左右。结论渝东北山区野葛最佳采集时间在11月〜次年3月。[关键词]野葛;葛粉;总黄酮;含量测定[中图分类号]R629[文献标识码]A[文章编号]1673-7210(2014)02(c)-0087-03StudyontheregulationofstarchandtotalflavonedynamiccontentinPuerarialobata(Willd.)Ohwifromnorthea
3、stmountainwildofChongqingTANXiaoyanlLIDabin2LINZhongxianglHUANGJing3JIAHaniWANGLiqingl1.ChongqingThreeGorgesMedicalCollege,Chongqing404020,China;2.WuxiCountyPeople,sHospital,Chongqing405800,China;3.WestChina.SchoolofPharmacy,SichuanUniversity,SichuanProvince,Chengdu610041,China[Abstract]Obje
4、ctiveToprovidescientificbasisfordeterminingtheoptimumharvesttime,byassayingandsummaryingthecontentofstarchandtotalflavoneinPuerarialobata(Willd.)OhwifromnortheastmountainwildofChongqingandthechangeregulationwiththeseasoninayear・MethodsPuerarialobatasampleswerecollectedeverymonthfromJune2011t
5、oMay2012innortheastofChongqingareaonschedule・Sampleswereslicedinto2-3mm,anddriedatroomtemperature・Accordingtoliterature,tissuesmashandwatersedimentmethodwasusedtodeterminethecontentofPuerariastarch・ThetotalconcentrationofflavoneinPuerarialobatasamplescouldbeevaluatedbyUV-visspectrumat250nm,u
6、singpuerarinascontrols,whichcaninferthechangeregulationofstarchandflavoneinPuerarialobatasamplesalongwiththealternatingofseasons・ResultsTheexperimentaldeterminationshowedthatthecontentofstarchandflavoneinPuerarialobataremainedaconstanttrendthroughoutayear.Generally,starchmaintainedbetween8.9
7、%~25・3%,andkeptmosthighfromNovembertoFebruary,thenMarch,August,SeptemberandOctober.ThelowestcontentwasfromApriltoJuly.Flovonewentsimilarity,whichkeptbetween2.13%-11.6%.Itwenthigherthan9%fromNovembertoMarch,remained6%duringAugustandOctoberandlowerth