vector nti使用介绍

《vector nti使用介绍》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。

1、MoleculeConstructionandDesignUsingVectorNTI10AdvanceYi-BuChen,Ph.D.BioinformaticsSpecialistNorrisMedicalLibraryUniversityofSouthernCalifornia323-442-3309yibuchen@belen.hsc.usc.eduWorkshopOutlineOverviewofmoleculetypesandcreationmethodsMajorstepsandtoolsformoleculeconstr

2、uctionanddesignConstructanewDNAmoleculeDesignanewDNAmoleculeOverviewofGatewayandTOPOcloningUsingsimulatedgelelectrophoresistoanalyzeclonedproductsUsefulonlineresourcesformoleculedesignpurposeFundamentalMoleculeTypesinVectorNTIBasicMolecules(DNA/RNA/Protein)☼Notbuiltfrom

3、componentfragments☼Sequencesandfeaturesareeitherenteredbyusersorimportedfromotherdatabases.ConstructedDNA/RNAMolecules☼Builtfromoneormorefragments(parentmolecules,linkers,adapters,etc.)☼AutomaticallyreceivetheFeaturemapandsequencesfromtheparentmolecules.ConstructedProte

4、inMolecules☼TranslatedfromacodingsequenceofaDNAmolecule☼DoesnotreceivetheFeaturemapfromitsparentDNAmoleculeMethodsofCreatingNewMoleculeinVectorNTIBasicMolecules(DNA/RNA/Protein)☼Importingmoleculesorsequences☼ManuallycreatingnewmoleculesConstructedDNA/RNAMolecules☼Splici

5、ngexonsofanintron-exonjoinfeatureofanothermolecules☼Constructingfromcompatiblecomponentfragmentsfromothermolecules☼Designingfromcomponentsofauser-definedfragmentslist☼Back-translatingfromproteinmoleculesfromcomponentsofauser-definedfragmentslistConstructedProteinMolecul

6、es☼Splicingexonsofagene☼TranslatedfromDNAmoleculesMoleculeConstructionvs.MoleculeDesignRecipientanddonorfragmentsaredefinedbyusers.Restrictionsitesaredefinedbyusers.Whenrequired,themethodsofterminusmodificationaredefinedbyusers.Recipientanddonorfragmentsareselectedbyuse

7、rs.RestrictionsitesareanalyzedandgeneratedbyVectorNTI.MethodsofterminusmodificationaredeterminedbyVectorNTI.MajorToolsforMolecularConstructionandDesignFragmentWizard☼Constructfragments(definepositionsandtermini)☼Designrecipient/donorfragments(definetermini)☼Adddefinedfr

8、agmentstotheGoalMoleculeDefinitionListTheGoalMoleculeDefinitionList☼Containsthelistoffragmentsdefinedandaddedb