免疫抑制剂对小鼠腹腔巨噬细胞的影响论文

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1、免疫抑制剂对小鼠腹腔巨噬细胞的影响论文.freelg/只，环磷酰胺2.0mg/只.freelethasoneandcyclphosphamideonperitonealmacrophageofmice【Abstract】Objective)inducedbyinjectionofdexamethasoneandcyclophosphamideinmice.MethodsTheimmunefunctionbydeterminingthephagocyticfunctionandstructureofthemacrophagocytesoftheabdomina

2、lcavitiesofmiceinvitro.Thephagocytosisofperitonealmacrophagesethod;ObservedtheconcentratuonofNO-2/NO-3intheperitoneallavagefluid.ResultsDexamethasoneandcyclophosphamideinducedtheapoptosisofperitonealmacrophageandconitantdecreaseofphagocytosis(vscontrolgroup，P0.01)，theconcentratuono

3、fNO-2/NO-3intheperitoneallavagefluidsignificantlyincreasedafterdexamethasoneinjection;VitC(inhibitorofiNOSandinhibitorofreactiveoxygenspecies)preventedtheapoptosisofperitonealmacrophageandreducedtheconcentration　　免疫抑制剂对小鼠腹腔巨噬细胞的影响论文.freelg/只，环磷酰胺2.0mg/只.freelethasoneandcyclphospham

4、ideonperitonealmacrophageofmice【Abstract】Objective)inducedbyinjectionofdexamethasoneandcyclophosphamideinmice.MethodsTheimmunefunctionbydeterminingthephagocyticfunctionandstructureofthemacrophagocytesoftheabdominalcavitiesofmiceinvitro.Thephagocytosisofperitonealmacrophagesethod;Ob

5、servedtheconcentratuonofNO-2/NO-3intheperitoneallavagefluid.ResultsDexamethasoneandcyclophosphamideinducedtheapoptosisofperitonealmacrophageandconitantdecreaseofphagocytosis(vscontrolgroup，P0.01)，theconcentratuonofNO-2/NO-3intheperitoneallavagefluidsignificantlyincreasedafterdexame

6、thasoneinjection;VitC(inhibitorofiNOSandinhibitorofreactiveoxygenspecies)preventedtheapoptosisofperitonealmacrophageandreducedtheconcentrationofNO-2/NO-3intheperitoneallavagefluidaftertheeffectofdexamethasone.ConclusionTheseevidencessupportthatNOandactiveoxygenspeciesmaybeinvolvedi

7、ntheapoptosisprocessofperitonealperitonealmacrophageinducedbydexamethasoneinmice.【Keyethasonecyclophosphamidemacrophage地塞米松和环磷酰胺在临床肿瘤的治疗中是常用的药物，在杀伤肿瘤细胞的同时又会引起免疫系统损伤，而影响治疗效果。巨噬细胞(macrophage，M)是机体免疫体系中重要的免疫效应细胞，具有非特异性直接清除各种异物，杀伤病原体和肿瘤细胞的功能，并具有抗原提呈作用。本实验选用小鼠腹腔巨噬细胞进一步探讨在整体条件下地塞米松和环磷酰胺对

8、免疫功能影响的机制。1材料和方法1.1实验分组及动物