常用缓冲液配制方法

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1、常用缓冲液的配制方法常用缓冲液的配制方法1.甘氨酸-盐酸缓冲液（0.05M）Xml0.2M甘氨酸+Yml0.2M盐酸再加水稀释至200mlpHX/mlY/mlpHX/mlY/ml2.25044.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸分子量＝75.070.2M甘氨酸溶液含15.01g/L2.邻苯二甲酸-盐酸缓冲液（0.05M）Xml0.2M邻苯二甲酸氢钾+Yml0.2M盐酸再加水稀释至200mlpHXYpHXY

2、2.254.6703.251.4702.453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.052.032邻苯二甲酸氢钾分子量＝2.4.230.2M邻苯二甲酸氢钾溶液含40.85g/L3.磷酸氢二钠-柠檬酸缓冲液pH0.2MNa2HPO4/ml0.1M柠檬酸/mlpH0.2MNa2HPO4/ml0.1M柠檬酸/ml2.20.4019.65.210.729.282.41.2418.765.411.158.852.62.1817.825.611.

3、608.402.83.1716.835.812.097.913.04.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.731.274.89.

4、8610.147.819.150.855.010.309.708.019.450.55Na2HPO4分子量＝141.980.2M溶液含28.40g/LNa2HPO4·2H2O分子量＝178.050.2M溶液含35.61g/LC6H8O7·H2O分子量＝210.140.1M溶液含21.01g/L4.柠檬酸-氢氧化钠-盐酸缓冲液pH钠离子浓度/M柠檬酸C6H8O7·H2O/g氢氧化钠NaOH/g浓盐酸HCl/ml终体积/L2.20.2021084160103.10.2021083116103.30.2

5、021083106104.30.202108345105.30.3524514468105.80.45285186105106.50.3826615612610使用时可以每升中加入1g酚，若最后pH有变化，再用少量50%氢氧化钠溶液或浓盐酸调节，冰箱保存。5.柠檬酸-柠檬酸钠缓冲液（0.1M）pH0.1M柠檬酸/ml0.1M柠檬酸钠/mlpH0.1M柠檬酸/ml0.1M柠檬酸钠/ml3.018.61.45.08.211.83.217.22.85.27.312.73.416.04.05.46.413

6、.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48.66.42.018.04.610.39.76.610418.64.89.210.8C6H8O7·H2O分子量＝210.140.1M溶液含21.01g/LNa3C6H5O7·2H2O分子量＝294.120.1M溶液含29.41g/L6.乙酸-乙酸钠缓冲液（0.2M）pH0.2MNaAc/ml0.2MHAC/mlpH0.2

7、MNaAc/ml0.2MHAC/ml3.60.759.254.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.657.355.48.601.404.43.706.305.69.100.904.64.905.105.89.400.60NaAc分子量＝136.090.2M溶液为27.22g/L7.磷酸盐缓冲液(1)磷酸氢二钠-磷酸二氢钠缓冲液（0.2M）pH0.2MNa2HPO4/ml0.2MNaH2PO4/mlpH0.2MNa2HP

8、O4/ml0.2MNaH2PO4/ml5.88.092.07.061.039.05.910.090.07.167.033.06.012.387.77.272.028.06.115.085.07.377.023.06.218.581.57.481.019.06.322.577.57.584.016.06.426.573.57.687.013.06.531.568.57.789.510.56.637.562.57.891.58.56.743.556.57.993.07.06.849.5