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1、1.20mMMOPSBuffer,pH7.0称取4.12gMOPS,加入无水醋酸钠0.656g,加水800mL溶解搅拌溶解;用2MNaOH(80g/L)调节pH至7.0。再加入EDTA0.5845g,加纯化水定容至1L。1.甘氨酸–盐酸缓冲液(0.05mol/L)6Q)w#m3E4?+V2^1C,jX毫升0.2mol/L甘氨酸+Y毫升0.2mol/LHCI,再加水稀释至200毫升!d:cG5c0u-x"ppH%a(Z9p$?5e"O*T!?5t#H&hXY+r.V1V.L,]:V3H%QpHX5E&Y-x(x3?Y/y9m,t4p
2、4B,J(H!K8N2.02.42.62.89p:]6z*A+p4B:S5050,v.T+a$Z$u,Z]0K+[50/a/W4v$m*t7B/n$R.z;d5044.0!
3、9u#K+k#vq1K-T%q32.4%n*A#H3[&f)x5y24.216.8.Ri:g5U&[3.0:Q.}6C$T(}$N9C:y3.23.4$k.X-d-C4V)m#X)y1A.K3.61X5N'L:_;R'@)u,?1T,L:q(q50%N)N1I#n1L1X#l5050{,H'h'I0c5011.4/H&~7n7J:l9Y8.26.4(a7T&H
4、!w6W0F/R)d5.0甘氨酸分子量=75.07,0.2mol/L甘氨酸溶液含15.01克/升。1l8h4x"^;{4
5、,~7n;e-^7k*c"C"C~/T2.邻苯二甲酸–盐酸缓冲液(0.05mol/L)&c:B+s0b/D*Q(RX毫升0.2mol/L邻苯二甲酸氢钾+0.2mol/LHCl,再加水稀释到20毫升2U/h;?;~'w9R3{!_8s2f3O2WpH(20℃)*o+L7]5E$Y7C%y)f)d6g+HXY$Z2v.w9w%P-w!UpH(20℃)X%S2S*v0g)?*F%xY2.22.4!q:s4v:oo/C
6、2.62.8,E(N3~*C8x,P/O:Y3.055;j,k:2g3t%n8a1b:A5%s(q5R#o7H+@;K550{6M/J,i9V4.070&g.y#{#a~5z[;~3.960:t#d'L7`1W5u9i&G3.2952.6422.0226R4?4I:s5h2~3.23.47w/w:D$P(L3.6,M1f,m:g,N3C6e.q3.855'_%h8e"A)R.A54r,]1[8Q2f&W6w*B5x5-N"v+A$};C2u~1.4700.990#H7x"X2W0.597+n)_6V2m3R"B"o-Y%j0
7、.263(O9t(H(g)W邻苯二甲酸氢钾分子量=204.23,0.2mol/L邻苯二甲酸氢溶液含40.85克/升;C3_8O"]"I%g)`0F5o8{/y.f(@7tK'W)k3.磷酸氢二钠–柠檬酸缓冲液(}5R&^0o7G;I7B%UpH0.2mol/LNa2HPO4(毫升)0.1mol/L"H6Z6z;!v柠檬酸pH2n(?5D!v"e0.2mol/LNa2HPO4*O:~9m)E-t7t+0.1mol/L柠檬酸"^4i1V7J6Z)i;d(毫升)u:AN(毫升)(毫升),X._8]3^$t+}2.2,c6A:g4U
8、%[4d1N2.42.68F9C3R#i8m$@$}9G;a2.83.03.23.43.64O&V(VS$x:l4`){3.84.06b#Y8_!I7h(p(f6a,C8j6R4.22Z-c0@"_7j3U6S(L4.44.64.8,P$X9T"X'u/Y*w5.06P%^(K2N$N6o0C8d;K&P:k0.40'm4_*k!T)w1.242.183.174.114.945.70:T/j+[0L)x6.447.10/Z.{0w6M)z"O7.718.288.829.35%?.U(g1l+C!o9`9.8610.300l)P9z
9、'N2h10.600b,R/^*I4P-T3[18.76%`,U%T*y7o+M17.8216.833T'y%f*~#L/{15.89'[0h7{,{,L3t2@15.0614.305Q+
10、5P3s8o+p(Y0i;R13.56:]+Z7i,N8F8[7t%q12.9012.2911.72&y/U4}2]&b-]11.18:[8f,J1M:_10.659t1}:e!x0K$z9O0l10.149.705.25.4!h+n*a2K%_X&J5Z"u2W5.66?-?'m;V%J8^5.86.06.2!I(v%A7u(N/w1T*E4
11、a6.4;d;Ho/g2U(r:]4y6.66.87.09U"p9U2o;L$I%s7.27.48[7m;u#@)t.]7.67.88.010.7211.15#C)C0c)W;Q9A11.6012.099l+J*c/Y;y6B.J%s12.631E