中山大学细胞生物学英文题库

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1、中山大学细胞生物学英文题库1.Youthinkyoumighthavediscoveredaneworganelleusingelectronelectronmicroscopy,butyouarenotsureifit'srealoranartifactofyourtechnique.Whatmightyoudotoproveitisreal?a.Lookforthestructureinmanysimilarlypreparedspecimensfromthesamesource.b.Lookforthestructur

2、einmanysimilarpreparedspecimensfromdifferentsources.c.Lookforthesamestructureinspecimenspreparedusingdifferentstainingprotocols.d.Lookforthesamestructureinspecimenspreparedusingdifferent(orno)fixatives.2.Traditionalgeneticapproachestoproblemsofcellbiologyhavefollow

3、edthepathofinformationfromgenetoprotein.BiochemicalapproacheshavetracedproteinsbackltotheDNAthatencodesthem,i.e.,fromproteintogene.Thischapterhasgivenyouthebasicstofolloweitherpath.Assumeyouarestudyingaproblemrelatingtocellfunction.Brieflyoutlinethebasicstepsyouwou

4、ldtakeifyouwerestartingwithageneandtryingtodeducethefunctionoftheprotein.Nowoutlinethestepsthatyouwouldtaketogetfromaspecificproteintoasequenced,isolatedgene.1.Indicatewhetherthefollowingstatementistrueorfalse.Ifitisfalse,expolainwhyinNuclease-chypersensitivesitesi

5、nchromationarelocatedinthelinkerDNAbetweennucleosomes.(F)2.Shortanswera.Evolutionaryconservationofhistoneproteins.b.nuclearmatrix3.Supposeyoudiscoveratemperature-sensitivenutantwhosenucleusfailedtoaccumulatecertainnuclearproteinatanelevated(restrictive)temperature,

6、butcontinuedtoaccumulateotherspecies.Whatconclusionsmightyoudrawaboutnuclearlocalizationandthenatureofthismutation?CellspossessmorethanoneNLS.receptorthatrecongnizedifferentlocalizationsequences,andthatthemutationwasoccuringinagenethatencodedoneoftheseNLSreceptorsp

7、ecies.1.Fillintheblanks.AfunctionalchromosomeregrirestheDNAsequenceelementatleastone_______topermitthechromosometocopiedonetofacilitatepropersegregationofitstwocopiesatmitosisandtwo________toallowthechromosometobemaintianedbetwiincellgenerations.(ARS;CEN)2.Whichsta

8、temintbestcharacterizesthenuclearlocalizationsignal?a.TheNLSistupicallyasmallmolecularweightmetabolicintermediate.b.TheNLSisashetchofhydrophobica