中山大学细胞生物学英文题库

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1、中山大学细胞生物学英文题库1.Youthinkyoumighthavediscoveredaneworganelleusingelectronelectronmicroscopy,butyouarenotsureifit'srealoranartifactofyourtechnique.Whatmightyoudotoproveitisreal?a.Lookforthestructureinmanysimilarlypreparedspecimensfromthesamesource.b.Lookforthestructureinmanysimilarprepa

2、redspecimensfromdifferentsources.c.Lookforthesamestructureinspecimenspreparedusingdifferentstainingprotocols.d.Lookforthesamestructureinspecimenspreparedusingdifferent(orno)fixatives.2.Traditionalgeneticapproachestoproblemsofcellbiologyhavefollowedthepathofinformationfromgenetoprotei

3、n.BiochemicalapproacheshavetracedproteinsbackltotheDNAthatencodesthem,i.e.,fromproteintogene.Thischapterhasgivenyouthebasicstofolloweitherpath.Assumeyouarestudyingaproblemrelatingtocellfunction.Brieflyoutlinethebasicstepsyouwouldtakeifyouwerestartingwithageneandtryingtodeducethefunct

4、ionoftheprotein.Nowoutlinethestepsthatyouwouldtaketogetfromaspecificproteintoasequenced,isolatedgene.1.Indicatewhetherthefollowingstatementistrueorfalse.Ifitisfalse,expolainwhyinNuclease-chypersensitivesitesinchromationarelocatedinthelinkerDNAbetweennucleosomes.(F)2.Shortanswera.Evol

5、utionaryconservationofhistoneproteins.b.nuclearmatrix3.Supposeyoudiscoveratemperature-sensitivenutantwhosenucleusfailedtoaccumulatecertainnuclearproteinatanelevated(restrictive)temperature,butcontinuedtoaccumulateotherspecies.Whatconclusionsmightyoudrawaboutnuclearlocalizationandthen

6、atureofthismutation?CellspossessmorethanoneNLS.receptorthatrecongnizedifferentlocalizationsequences,andthatthemutationwasoccuringinagenethatencodedoneoftheseNLSreceptorspecies.1.Fillintheblanks.AfunctionalchromosomeregrirestheDNAsequenceelementatleastone_______topermitthechromosometo

7、copiedonetofacilitatepropersegregationofitstwocopiesatmitosisandtwo________toallowthechromosometobemaintianedbetwiincellgenerations.(ARS;CEN)2.Whichstatemintbestcharacterizesthenuclearlocalizationsignal?a.TheNLSistupicallyasmallmolecularweightmetabolicintermediate.b.TheNLSisashetchof

8、hydrophobica