screen+and+effect+analysis+of+immunostimulants+for+sea+cucumber,+apostichopus+japonicus

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1、ChineseJournalofOceanologyandLimnologyVo1．27No．1．P80—84．2009DoI：10．1O07／s00343．009．O080．5Screenandeffectanalysisofimmunostimulantsforseacucumber，Apostichopusjaponicus冰LIJiye(李继业)，什，SUNXiuqin(孙修勤)卅’，ZHENGFengrong(郑风荣)"叶，⋯HAOLinhua(郝林华’CollegeofMarineLScience，O

2、ceanUniversity'ofChina，Qingdao266003，ChinaNorthChinaSeaEnvironmentalMonitoringCenter,StateOceanicAdministration，Qingdao266033，China一”TheFirstInstituteofOceanograph~SOA，Qingdao266061，ChinaReceivedJune18，2008；revisionacceptedOct．8，2008AbstractImmunostimulantsma

3、yimprovediseaseresistanceofaquacultureanimalsbypromotingthenonspecificimmunityresponseoftheorganisms．Fivetypesofsaccharides，includingchitosan，yeastpolysaccharide，burdockoligosaccharide，seaweedpolysaccharideand1entinusedodespolysacchalnde，werescreenedforpotent

4、ialuseasimmunostimulantsbyusingspectrophotometry．Thesaccharideswerei~ectedintoApostichopusjaponicus，aseacucumber,andthelysozymeandsuperoxidedismutase(SOD)activitiesofthecoelomicfluidandepidermalslimeweremonitoredinsixconsecutivedays．Theresultsshowthatthe1ysoz

5、ymeactivityoftheanimal’scoelomicfluidwassignificantlystimulatedofday2．day4andday6aftertheiniectionofthesaccharides(P<0．05)，Theefectsofchitosanandyeastpolysaccharidewerethemostnotable．Thelysozymeactivityoftheepidermalslimewassignificantlyincreasedbychitosana，y

6、eastpolysaccharide，seaweedpolysaccharide，andburdockoligosaccharideonday1andday2aftertheiniectionrP<0．05)．TheSODactivityofthecoelomicfluidwassignificantlypromotedbythesaccharidesonday2andday4post—injection(P

7、donday2．Thesefindingsindicatethatchitosanandyeastpolysaccharidearethemostefectiveimmunostimulantsandpotentia1healthyanti—diseasefeedstufforA．iaponicus．Keyword：Apostichopusjaponicus；immunostimulant；lysozyme；superoxidedismutaselINTRODUCTIONurgentlyneededforfind

8、ingeffectivecures．Immunostimulant，alsonamedschlepper，mayApostichopusjapomcus，aspeciesotseaimprovediseaseresistanceoftheorganismsbycucumber，iseconomicallyimportantinmariculturepromotingori