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1、生物化学与分子生物学实习指导（PracticeinstructionofBiochemistryandmolecularbiology）GuidanceforBiochemistryandmolecularbiologypractice(Chineseversion)PracticeinstructionofBiochemistryandmolecularbiologyExperiment1.Determinationofproteincontent(colorimetricanalysisandspectrophotometry)Inexperi
2、menttwo,DNAwasextractedfromliverofmiceExperimentthreeDNAenzymatichydrolysisandDNAfragmentagarosegelelectrophoresisExperimentfourmodernPCRTechnologyIsolationandidentificationofserumantitrypsin(alpha1AT)infiveexperimentalsubjectsContentonesaltingoutmethod,gelfiltrationmethodCont
3、enttwocelluloseionexchangechromatography,determinationofproteincontent,determinationofenzymeactivityContentthreeSDS-polyacrylamidegelelectrophoresisContentfoursummaryInexperimentsix,thespecificity,agonistandinhibitorofenzymewereobservedEffectoftemperatureandpHonenzymaticreacti
4、onspeedinexperimentsevenEffectofexerciseeightonthecontentoflacticacidinurineExperimentnineliverglycogentestExperimenttenextractionandthin-layerchromatographyofliverlipidsExperiment1.Determinationofproteincontent(colorimetricanalysisandspectrophotometry)[objectiverequirements]1
5、.masteringthebasicprincipleofcolorimetricanalysisandspectrophotometry,makingandusingthestandardcurve,andcalculatingtheresultofstandardtubemethod2.familiarwithcommonmethods,principlesandapplicationsofproteincontentdetermination3.tounderstandtheprincipleandapplicationofthecommon
6、lyusedvisibleandultravioletspectrophotometer[teachingcontent]Determinationofproteincontentby1.phenolreagentmethodLambert-Beerlaw,colorimetricmethodandbasicprincipleofspectrophotometry,722spectrophotometer,methodandapplicationofdeterminationofproteincontent,proteincontentdeterm
7、ination,theoperationprincipleofphenolreagentmethod,standardcurvepreparationanduseDeterminationofproteincontentof2.biuretmethodTheexperimentwascompletedindependentlyandindependently,andcomparedwiththephenolreagentmethod3.determinationofproteincontentbyUltravioletSpectrophotomet
8、ryProteindeterminationprinciple,UVspectrophotometrymethod,adv
