猪瘟和蓝耳病疫苗中牛病毒性腹泻病毒污染情况的检测与分析毕业论文

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1、青岛农业大学毕业论文题目：猪瘟和蓝耳病疫苗中牛病毒性腹泻病毒污染情况的检测与分析姓名：南76767学院：动物科技学院专业：动物医学专业班级：1003学号：20103637指导教师：韩先杰2014年5月21日目录中文摘要······································································································IAbstract······························

2、····························································II引言································································································21材料与方法21.1试验材料21.1.1试验疫苗21.1.2试验试剂21.1.3试验主要仪器设21.2试验方法31.2.1RNA的提取31.2.2RT-PCR检测BVDV31.2

3、.3巢式PCR扩增41.2.4琼脂糖凝胶电泳41.2.5胶回收提纯41.2.6回收产物连接51.2.7转化反应51.2.8阳性克隆筛选61.3序列分析62结果与分析72.1PCR扩增结果72.1.1巢式PCR扩增结果72.1.2菌液PCR结果82.2测序结果92.3序列分析112.3.1进化树分析112.3.2同源性比较123讨论133.1检测方法133.2BVDV的污染情况133.3控制疫苗中的BVDV污染措施143.3.1预防免疫143.3.2注意制作工艺143.3.3国家加强对疫苗的监控与

4、净化144小结14致谢············································································································15参考文献······································································································16猪瘟和蓝耳病疫苗中牛病毒性腹泻病毒污染情况

5、的检测与分析动物医学专业南福龙指导教师韩先杰摘要：为调查我国商品化猪瘟和蓝耳病疫苗中牛病毒性腹泻病毒污染情况，本文采集179份蓝耳病疫苗和105份猪瘟活疫苗，用RT-PCR方法检测疫苗中的牛病毒性腹泻病毒，评估疫苗污染牛病毒性腹泻病毒的情况。结果表明，蓝耳病疫苗中检出6份阳性样品，总污染率为3.6%；猪瘟疫苗中检出3份阳性样品，总污染率2.9%，造成污染的病毒均为牛病毒性腹泻病毒1型。关键词：牛病毒性腹泻病毒；PCR；猪瘟；猪蓝耳病；疫苗IDetectionandAnalysisofBovine

6、ViralDiarrheaVirusPollutioninClassicalSwineFeverandPorcineReproductiveandRespiratorySyndromeVaccinesStudentmajoringinAnimalMedicineNanFulongTutorHanXianjieAbstract:ToinvestigatethecontaminationsituationofBVDV,105CSFvaccinesamplesand179PRRSvaccinesamp

7、leswerecollected.Then,RT-PCRwasappliedtodetectBVDVandevaluatetheconditionofpollutioninvaccines.Theresultsshowed6ofthe179PRRSvaccinesampleswereBVDVpositiveandthecontaminationratewas3.6%.Atthesametime,3ofthe105CSFvaccinesampleswereBVDVpositiveandthecon

8、taminationratewas2.9%.AllthedetectedBVDVwereidentifiedasBVDVtype1.Keywords:BovineViralDiarrheaVirus;PCR;ClassicalSwineFever;PorcineReproductiveandRespiratorySyndrome;Vaccine16目前，我国猪群重大疫病的防控主要依赖疫苗免疫，如猪瘟、蓝耳病等。弱毒疫苗具有可诱导体液和细胞双重免疫、只接种一次即可起到良好的预防效果、持续时间长、不