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ID:11745134
大小:46.50 KB
页数:18页
时间:2018-07-13
《痛泻要方对ibs模型血清内源性物质代谢干预实验探究》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、痛泻要方对IBS模型血清内源性物质代谢干预实验探究 [摘要]通过代谢组学评价痛泻要方对肠易激综合征(irritablebowelsyndrome,IBS)大鼠模型血清内源性物质代谢干预效应,寻找潜在的生物标志物并分析其代谢途径,探讨痛泻要方的作用机制及病证模型的证候本质。将40只Wistar大鼠复制为IBS模型,随机分为模型对照组和痛泻要方给药组4组,另设空白对照组10只。痛泻要方(低、中、高)组分别灌胃剂量为0.203,0.406,0.812g・mL-1的痛泻要方,空白对照组及模型对照组给予等体积的生理盐水,每日1次,连续2周。各组大鼠于灌胃第0,15天采集血清
2、,经处理后供UPLC-Q-TOF-MS进行代谢组学分析。鉴定出8个潜在生物标志物,分析出8条主要代谢通路,其与IBS疾病的神经递质代谢、炎性免疫、脑神经功能及能量代谢等有关,痛泻要方对IBS疾病的作用机制可能涉及血清素突触和色氨酸代谢、半胱氨酸和甲硫氨酸代谢、甘油磷脂代谢、烟酸和烟酰胺代谢等过程,其可能是IBS模型肝旺脾虚证的生物学基础[关键词]痛泻要方;肠易激综合征;肝旺脾虚;内源性代谢物;代谢组学[Abstract]ToevaluatetheeffectofTongxie18Yaofangoncardiacendogenousmetabolisminirrita
3、blebowelsyndrome(IBS)ratsbyusingmetabolomicsmethod,finditspotentialbiomarkers,analyzethemetabolicpathways,andexplorethepharmacologicaleffects,mechanismsofactionandsyndromeessenceofsyndromemodel.FortyWistarratswereusedtoestablishIBSmodels,andthenrandomlydividedintofourgroups:modelcontro
4、lgroupandTongxieYaofangtreatmentgroups(high,medium,lowdose).Another10ratswereusedasnormalgroup.TheratsinTongxieYaofang-treated(low,mediumandhighdose)groupswereorallyadministratedwithTongxieYaofangextractsonceadayfor2weeks,respondinglywiththedosesof0.203,0.406,0.812g・mL-1.Theratsinnorma
5、lgroupandmodelcontrolgroupweregivenwithequalvolumeofsalineonceadayfor2weeks.Onthe0and15thdays,serumwascollectedandeachsampleextractwasanalyzedbyUPLC-Q-TOF-MS.Eightpotentialbiomarkerswereidentifiedand8majormetabolicpathwayswerefoundtoberelatedwithIBSdiseasesneurotransmittermetabolism,in
6、flammatoryimmunity,18brainfunctionandenergymetabolism,etc.TongxieYaofanghadcertainpharmacologicaleffectsonIBS,anditsmechanismmayberelatedtoserotonergicsynapse,tryptophanmetabolism,cysteineandmethioninemetabolism,glycerophospholipidmetabolism,nicotinateandnicotinamidemetabolismandsoon,w
7、hichmightbethebiologicalbasisofIBSliver-spleendeficiencysyndrome.[Keywords]TongxieYaofang;IBS;liverstagnationandspleendeficiency;endogenousmetabolites;metabolomics本研究�M在前期工作中已进行了较深入的痛泻要方对肠易激综合征(irritablebowel18syndrome,IBS)大鼠模型脑-肠互动效应机制影响的研究[1-2],并对其作用机制有一定阐释。对机体、病证的作用机制仅通过某些作用靶点、机制、
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