枸杞多糖及丹参酮延缓小鼠皮肤衰老实验探究

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1、枸杞多糖及丹参酮延缓小鼠皮肤衰老实验探究【摘要】目的比较枸杞多糖与丹参酮对衰老小鼠皮肤的抗衰老作用。方法60只雌性昆明种小鼠，随机分为4组(n=15)：正常对照组皮下注射生理盐水；其余3组每日颈背部皮下注射D-半乳糖［1000mg/(kg•d)］造成小鼠衰老模型，同时各组分别灌胃生理盐水(衰老模型组)、枸杞多糖［200mg/(kg•d)］、丹参酮［1500mg/(kg•d)］。42d后，测定小鼠背部皮肤组织匀浆中超氧化物歧化酶(SOD)活力、过氧化脂质代谢产物丙二醛(MDA)

2、含量和皮肤羟脯氨酸(Hyp)含量。结果枸杞多糖可使衰老模型小鼠皮肤中MDA含量显著降低,较丹参酮组明显；但SOD活力显著升高,不及丹参酮组改变显著；枸杞多糖和丹参酮均可使羟脯氨酸含量升高，且效果相当。结论枸杞多糖与丹参酮都有显著的抗小鼠皮肤衰老作用，枸杞多糖清除皮肤中脂质过氧化代谢产物方面较丹参酮明显,而提高皮肤抗氧化酶活力则不及丹参酮显著，在增强成纤维细胞活性方面两者效果相当。【关键词】皮肤衰老；枸杞多糖；丹参酮Experimentalresearchontheskinagingfunctionoftansh

3、inoneandlyciumbarbarum8polysaccharidesLIANGJie，LIXiang.DepartmentofPlasticSurgery，AffiliatedHospitalofGuangdongMedicalCollege,Zhanjiang,Guangdong524001，China【Abstract】ObjectiveTocomparetheanti-agingeffectsofLyciumbarbarumpolysaccharidesandTanshinoneontheskin

4、ofD-galactose-inducedmiceagingmodel．Methods60femalemicewererandomlydividedinto4groups(n=15):Miceinnormalcontrolgroupreceivedsubcutaneousinjectionofnormalsalineandmiceintheother3groupsreceivedcervicodorsalinjectionofD-galactose［1000mg/(kg1•d)］for42dtoes

5、tablishsubacuteagingmodel.Theexperimentalgroupsincludedagingmodelgroup(withoutfurthertreatment)，Lyciumbarbarumpolysaccharides［200mg／(kg•d)group，Tanshinone［1500mg／(kg•d)］group．Fortytwodayslater，thedorsalskinsampleswerecollectedtodetermineSODactivi

6、ties、MDAandhydroxyprolinecontents．ResultsTheeffectofLyciumbarbarumpolysaccharidesisbetterthanTanshinonesignificantlyindecreasethecontentofMDA，butin8increasetheSODactivityTanshinonehavebettereffection.Bothofthemcanincreaseingthehydroxyprolinecontents，butthese

7、isnodifferent.ConclusionBothLyciumbarbarumpolysaccharidesandTanshinonehavenoticeableanti-skinagingeffects，LyciumbarbarumpolysaccharidesisbetterthanTanshinoneinclearingskinlipidperoxidationmetabolitesaspects，butTanshinonehavebettereffectioninenhancingOxidasea

8、ctivity，theyhavenodifferenteffectioninenhanceingfibroblasts.【Keywords】Skinaging；Lyciumbarbarumpolysaccharides；Tanshinone当今社会由于老龄化的加速到来以及人们对美的强烈追求，希望借助医学手段延缓衰老，永保青春。枸杞为茄科植物枸杞成熟果实，明朝李时珍在《本草纲目》中记载：