DB440300T32.1-2007农业转基因生物食用安全性检验第1部分样品制备.pdf

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1、免费标准网(www.freebz.net)ICS65.020.01B04DB深圳市农业地方标准DB440300/T32.1—2007农业转基因生物食用安全性检验第1部分：样品制备Ediblesafetydeterminationofagriculturegeneticallymodifiedorganisms—PartI:Methodforsamplepreparation2007-11-12发布2008-02-12实施深圳市质量技术监督局发布免费标准网(www.freebz.net)无需注册即可下载免费标准网(www.fr

2、eebz.net)DB440300/T32.1—2007目次前言.................................................................................II1范围................................................................................12规范性引用文件..........................................................

3、............13术语和定义..........................................................................14DNA样品制备........................................................................15RNA样品制备........................................................................26蛋白质样品制备.

4、.....................................................................37外源基因表达产物制备................................................................3附录A（规范性附录）DNA样品制备....................................................5A.1DNA样品制备常用试剂及仪器................................

5、........................5A.2DNA提取方法......................................................................6附录B（规范性附录）RNA样品制备.....................................................8B.1RNA样品制备常用试剂及仪器.........................................................8B.2RNA提取方法...

6、....................................................................8附录C（规范性附录）蛋白质样品制备..................................................10C.1蛋白质样品制备常用试剂及仪器.....................................................10C.2蛋白质提取制备方法...........................................

7、....................10附录D（规范性附录）外源基因表达产物制备............................................11D.1外源基因表达产物制备常用试剂及仪器...............................................11D.2从转基因生物制备表达产物方法.....................................................12D.3工程菌的构建及培养方法......................

8、.....................................14参考文献.............................................................................16I免费标准网(www.freebz.net)无