肾上腺素对离体豚鼠左心室流出道组织自律性电活动的影响

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1、肾上腺素对离体豚鼠左心室流出道组织自律性电活动的影响：陈立锋徐涛范凌陈少峰孙琳　尹万斌　赵兰平【摘要】目的：观察肾上腺素(E)对离体豚鼠左心室流出道组织自发慢反应电位的影响。方法：采用标准玻璃微电极细胞内记录技术，观察E对离体豚鼠左心室流出道组织自发慢反应电位的影响。观测指标有：最大舒张电位(MDP)，动作电位幅度(APA)，0期最大除极速度(Vmax)，4期自动除极速度(VDD)，自发放电频率(RPF)，复极50%和90%时间(APD50、APD90)。结果：用100μmol/LE灌流，豚鼠左心室流出道自律组织动作电位

2、APA明显增大(P<0.01)，Vmax明显增快(P<0.05)，MDP显著增大(P<0.05)，APD90显著缩短(P<0.01);RPF明显增快(P<0.01)，VDD明显加快(P<0.05)。结论：E对左心室流出道组织自律细胞有兴奋作用。【关键词】肾上腺素;电生理学技术/心脏;豚鼠;左心室流出通;自律细胞Asisaclinicalcardiacstimulant,theepinephrine(E)hasveryimportantfunctioninthecardiovascula

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4、akercellsofGuineapig.Thepurposeofourstudyakercellsinleftventricularoutfloechanism(s)involved,provideelectrophysiologicproofforthenosogenesisofsomearrhythmiasthatoriginatedfromventricularoutfloenPreparation　　Guineapigofeithersex(entAnimalResearchCenterofChineseA

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6、mofaperfusionchamber[1,2].ThepreparationsL/min)(36.0±0.5)℃improvedLockesolution.Itaintainedat(36.0±0.5)℃icroelectrodetechniquetorecordAps,Thetransmembraneactionpotentialsol/LKClfilledglassmicroelectrode(atipresistanceof10～20MΩ)coupledtoahighinputimpedenceamplifi

7、er(madeinChengduInstrumenyFactory),Theamplifiedsignalsultichannelphysiologicalsignalacquisitionsystem(RM6280C,madeinChengduInstrumenyFactory)andprocessedbyamicroputer.Theparametersofactionpotentialsaticallyanalyzedbythemicroputer.　　1.3ElectrophysiologicalParamete

8、rs　　MDP(maximaldiastolicpotential),APA(amplitudeofactionpotential),Vmax(maximalrateofdepolarization),VDD(velocityofdiastolicdepolarization),APD50andAPD90(50%an