avastin抑制兔眼小梁切除术后滤过泡瘢痕化的作用

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1、Avastin抑制兔眼小梁切除术后滤过泡瘢痕化的作用：吕娟，赖铭莹，应方微，黄丽娜，成洪波【摘要】　　目的：探讨Avastin抑制兔眼小梁切除术后滤过泡纤维瘢痕形成的作用。方法：兔20只40眼随机分为3个不同剂量Avastin实验组、丝裂霉素C（MMC）对照组及空白对照组。对兔眼行常规小梁切除术，Avastin实验组于术毕及术后3，7d术区结膜下分别注射0.5，1及2mgAvastin；MMC对照组术中筋膜囊、巩膜瓣下放置0.2g/LMMC棉片。术后裂隙灯下观察滤过泡形态及角膜、前房等情况；术后7，14d摘除眼球，常规HE染色观察组织形态，在高倍视野中

2、，通过滤过泡腔面在结膜中形成的面积百分比及滤过泡形成区在结膜中面积百分比，评价不同剂量Avastin结膜下注射对滤过泡形成及纤维化的影响。结果：术后7d各组结膜纤维结缔组织中均可见明显的滤过泡形成。术后14d仅注射2mgAvastin实验组及MMC对照组的结膜组织中可见明显滤过泡；1mgAvastin实验组见少量面积微小的滤过泡；0.5mgAvastin实验组及空白对照组滤过泡均消失，纤维组织增生明显。术后7d各组高倍视野下滤过泡腔面在结膜中形成的面积百分比及滤过泡形成区在结膜中面积百分比无明显差异。术后14d，2mg及1mgAvastin实验组滤过泡

3、腔面积在结膜中形成的面积百分比分别为1.0%，0.8%，MMC组为0.9%。2mg及1mgAvastin实验组滤过泡形成区在结膜中面积百分比分别为26.1%，2.9%，MMC组为25.8%。2mg及1mgAvastin实验组的滤过泡腔面及形成区面积与结膜面积的百分比均明显高于空白对照组，单项方差分析结果分别为F=270.10,P=0.00；F=49.99,P=0.00；2mgAvastin实验组的上述滤过泡面积百分比与MMC组无差异。结论：结膜下注射Avastin可有效帮助滤过术后滤过泡的形成与维持，缓解滤过泡的纤维增生。【关键词】Avastin；滤过

4、泡；瘢痕化；小梁切除术；血管内皮生长因子　　AbstractAIM:ToevaluatetheefficacyofAvastinontreatingtheblebfibrosisaftertrabeculectomyinrabbits.METHODS:Fortyeyesof20rabbitsundergonetrabeculectomylydividedintothreedifferentdosesofAvastinexperimentalgroups,mitomycinC（MMC）groupandblankcontrolgroup.Theeyesi

5、nthreeAvastinexperimentalgroupslyreceivedsubconjunctivalinjectionof0.5，1，2mgAvastinyfinishedandon3rd,7thdaysaftersurgery.IntheMMCtreatedgroup，MMCcottonpatch，0.2g/L,matoryreactionsp;thefilterareasinationonpostoperative7，14days.RESULTS:Filterblebscouldbeseenclearlyineachgrouponpos

6、toperative7days.Andonpostoperative14daysinthe2mgAvastingroupandMMCgroup,filteringblebsoreelevatedthanothergroups;in1mgAvastingroup,thereesmallblebs;andin0.5mgAvastingroupandblankcontrolgroup,filterblebsdisappeared,andmarkedhyperplasiaoffibroustissuecouldbeseen.Underthemicroscope

7、onpostoperative7days,thereingregionintheconjunctiva.Onpostoperative14days,thepercentageoftheblebcavitysurfaceareaintheconjunctivaof2mgand1mgAvastingroupsandMMCgroupingregionintheconjunctivaingregionintheconjunctivaof2mgand1mgAvastingroupgAvastingroupandMMCgrouponthefilteringbleb

8、areapercentage.CONCLUSION:Subconjunctivalinject