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1、Borleeetal.SupportingInformationIdentificationofSyntheticInducersandInhibitorsoftheQuorum-SensingRegulatorLasRinPseudomonasaeruginosabyHigh-ThroughputScreeningBradleyR.Borlee,1±GrantD.Geske,3HelenE.Blackwell,3andJoHandelsman1,2ψ*DepartmentofPlantPathology1,DepartmentofBacter
2、iology2,andDepartmentofChemistry,3UniversityofWisconsin–Madison,Madison,Wisconsin53706±CurrentAddress:DepartmentofMicrobiology,UniversityofWashingtonSchoolofMedicine,1959NEPacificSt.,RoomK-360,Seattle,WA98195-7242.ψCurrentaddress:DepartmentofMolecular,CellularandDevelopmenta
3、lBiology,219ProspectSt.,YaleUniversity,NewHaven,CT06471*Correspondingauthor.Mailingaddress:DepartmentofMolecular,CellularandDevelopmentalBiology,219ProspectSt.,YaleUniversity,NewHaven,CT06471.Phone(203)432-9119.E-mail:jo.handelsman@yale.eduSupportingInformation.Supplementary
4、Table1.BacterialstrainsandplasmidsS-2SupplementaryTable2.AgonistinformationS-3SupplementaryTable3.AntagonistinformationS-3SupplementaryModelingResultsS-4SupplementaryFigure1S-5SupplementaryFigure2S-6SupplementaryFigure3S-6SupplementaryFigure4S-7SupplementaryFigure5S-7Supplem
5、entaryFigure5S-8SupplementaryFigure7S-8SupplementaryFigure8S-9SupplementaryFigure9S-9SupplementaryFigure10S-10SupplementaryFigure11S-10SupplementaryMethodsS-11SupplementaryReferencesS-13S-13Borleeetal.SupportingInformationSupplementaryTable1.BacterialstrainsandplasmidsStrain
6、orplasmidRelevantcharacteristicsaSourceorreference(seemaintext)PseudomonasputidaF117pKRC12AHL-deficientderivativeofP.putidaIsoF,∆ppuI.pKR-C12:pBBR1MCS-5carryingPlasB-gfp(ASV)-Plac-lasR;basedoncomponentsoftheP.aeruginosalasquorum-sensingsystem;GmR(9,10)PseudomonasputidaF117pK
7、RC12ΔlasRAHL-deficientderivativeofP.putidaIsoF,∆ppuI.pKRC12ΔlasR:pBBR1MCS-5carryingPlasB-gfp(ASV)∆Plac-lasRThisstudyEscherichiacoliMT102pJBA132MT102:araD139(ara-leu)7697∆lacthihsdR.pJBA132:pME6031carryingluxR-PluxI-RBSII-gfp(ASV)-T0-T1;basedoncomponentsoftheV.fischeriluxquor
8、um-sensingsystem;TetR(1)EscherichiacoliDH10BF-endA1recA1galUgalKdeoRnupGrps
