鸭疫里默氏杆菌大罐发酵培养条件的优化

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1、2014୍36ज22௹ChinaPoultryVol.36đNo.22.2014࿹࣮ࡥБ࿥ၦ৚ଏ൦ۆऩնܷ࢞஡အ่ࡱႪ߄*຦ဇđ૬৫ᇏđڱ఼đሌࣁ౬đഫᆽ఼č೶תസўᇜ྾ତ൵၄࿹࣮ჽđ೶תўᇜ256600Ďᅋေğູࣉ၂ਔࢳ࿥ၦ৚ଏ൦ۆऩളӉหྟđЧ࿹࣮০Ⴈڿਅ࢞ଛಽณ஡အࠎđᄎႨᆞࢌഡ࠹ᇕ۲ऩۆ൦ଏ৚ၦ࿥ؓᇏܷ࢞྘ཬL10ᄝđم่ٚ࢞ࡱࣉྛਔႪ߄bෛުࡼႪ߄ࢲۆ൦ଏ৚ၦ࿥஻3Ӂള߄൫ଆܷܿ࢞྘նL200Ⴈ০đࡗӚӁളPMGᄝݔऩॆჰࣉྛဒᆣđࢲݔཁൕđ࿥ၦ৚ଏ൦ۆऩࠃऩඔбႪ߄భิۚ30%đູ࿥ၦ৚ଏ൦ۆऩॆჰӁြ߄

2、׍קࠎԤbܱ࡯Սğ࿥ၦ৚ଏ൦ۆऩĠ่࢞ࡱĠնܷ࢞࿥ၦ৚ଏ൦ۆऩčRiemerellaanatipestiferđ20minđ൐Ⴈభࡆೆ5%ઔ࿓bRAĎۋಙ൞ಆൗࢸအ࿥ြ၂ᇕᇶေࠥѨđఘࣂ1.1.3ᇶေ၎ఖބ൫࠴ູᆸđݓႇđ྘E40-ABSč࠹ܻܻٳຓሬඏܻචᇕ2ᇕࢤ૬ၦࠣᇍٝ໾ူႵေᇶᇍٝѨھŀ1łĠĎඳ܄Cecilܱࢤᆰ୩ุऩđᇏӱݖСᇅ૬ၦᄝbمܷٚ࢞čGUJS-10C྘ބGUJS-200C༢ၦ૬ᇉਈߊބ૧ၦЌ޹ིݔb෮ၛڿ೿྘đᆓࡾתٚള໾۽ӱഡС࠯ඌႵཋᄳ಩܄ඳĎĠRA஡အ่ࡱิ٤Сᇅ૬ၦؓ୩ٳӮིႵۚగფޚ

3、໑ᆒఖčSHZ-82A྘đࡾ්സࣁฆ൧ᇏնӈᇗေbૡۚ࢞ቔ୍ູ࣍টྍྖӁြđॖ၎ఖӌĎĠཨஞ࠴Ġ׸ႲbႵིิمٚ2.1ࢆږնđੱႨ০ࠎအ஡ބਈӁჰॆۚളӁӮЧđཁᇷ෪؋ളӁᇛ௹đࡨഒস׮఼đ1.2.1ऩᇕگ්ŀ2łิۚളӁིੱđູ࿥ၦ৚ଏ൦ۆऩѨ૵ࠃၦ૬౼ײۄЌթ࿥ၦ৚ଏ൦ۆऩJX-2ᇩđႨളӁิ܂ਔ၂ᇕྍႵིॖྛॆჰᇅС۽ၜb2mLઔנಽณ༎൤aࠁᄋđࢤᇕႿڿਅ࢞ଛಽณЧ࿹࣮ᆻᄝ๙ݖ࿥ၦ৚ଏ൦ۆऩ࢞஡အ่ࡱႪᇏđ37℃ᆒ஡အ18~24hđ౼ဢ߃ཌࢤᇕႿڿਅ࢞߄ղऩ၁୩ቋն߄ଢđູ࿹ᇅིۚၦ૬ଛܥุ஡အࠎ௜ϰđᇂညသ

4、ܷᇏđ37℃஡အ18~24hđյࠎԤbᄜ็࿊ژކѓሙ྘ऩઋႨڿਅ࢞ଛܥุ஡အ1ҋਘაٚمࠎࣉྛՂ஡အb1.1ҋਘ1.2.2࢞༥ऩᇕሰ၁ᇅС1.1.1ऩᇩႨڿਅ࢞ଛಽณ༡༯Ղ஡အऩઋđο2%ᇕሰ࿥ၦ৚ଏ൦ۆऩJX-2ᇩđ࿓Ⅱ྘đٳ৖ሱਈࢤᇕႿ120mLڿਅ࢞ଛಽณ஡အࠎᇏđ37℃ᆒКࣘࣁྒ࿥ӆѨඵ࿥đѩႮ೶תੳ׻ള໾॓࠯܄஡အ18~24hđࣜՂե࡟ဒކ۬ުđ4℃ЌթСႨbඳऩᇕ൩Ќթb1.2.3ٚဒ߄൫Ⴊၜ۽࢞ૡۚσഡ࠹ŀ2ł1.1.2مٚ࠹ഡဒൌࢌᆞႨᄎğဒ൫ࢌᆞࠎအ஡࢞đࡼ႕ڿਅ࢞ଛಽณğն׸Ϣ᥻5gđඣࢳೄϢཙ࿥

5、ၦ৚ଏ൦ۆऩ่࢞ࡱٳູ๙గčL/S.LĎa8gđ20%࢞ଛ࣏၁50mLđ੯߄଩1.5gđᇬ໇ཨ߄ሇ෎čr/minĎa஡အࠎpHᆴđѩࡼ۲่ࡱٳљഡ၁100mLđࡆᆕ਼ඣᇀ1000mLĠ116℃ٳဒ൫ุऎđ௜ඣ3۱ᇂऩ૵࿢ۚቆ࡮і1bٳඍഈğ࢞ቆ൫ဒनᄝ37℃่ࡱ༯đ஡အࠎູڿਅ࢞ଛಽณđٳљοᅶഈඍ൫ဒ่ࡱ۲ሱ൬۠ರ௹ğ2014-02-14*๙࿟ቔᆀđE-mailğbzshenzq@vip.sina.com࢞đܷ࢞ҐႨ10Lಸਈđ࢞၁ູ6000mLđٳ-53-2014୍36ज22௹ChinaPoultryVol.36

6、đNo.22.2014࿹࣮ࡥБі2ݔࢲקҩUFCቆ۲ࡗൈ๝҂࢞108cfuahі1ٳဒ൫ࢌᆞࡱ่࢞ቆ౦ঃ൫ဒ౼ဢൈࡗၹ෍൫ဒ྽྽681012141618202224๙గčL/S.LĎሇ෎čr/minĎ஡အࠎpHᆴ16111715222325626926423521220610.11007.226512516624027528328727125322520.11507.436312215822726327226824523122130.12007.64499612117620921222422720218440.510

7、07.455410813016722423624222920819650.51507.665711215221724825526724322621060.52007.27438411117821622422621420118271.01007.68489313421522324124323221519381.01507.295510613317522823724522621720591.02007.4350љҐࠢ࢞ު6a8a10a12a14a16a18a20a22a24h300Ď࢞၁đࣉྛࠃऩ࠹ඔࠣOD450nmᆴ࡟

8、ҩbcfu2508102001.2.4Ⴊ߄஥ٚބႪ߄۽ၜᄝGMPളӁӚࡗܿଆč150߄ളӁႋႨ100ࠃऩඔ۴ऌ஡အࠎ஥ٚႪ߄ބ࢞۽ၜႪ߄ࢲݔđ500ᄝGMPളӁӚࡗܿଆ߄࢞ളӁ3஻࿥ၦ৚ଏ൦681012141618202224ളӉൈࡗčhĎۆऩॆჰđܷ࢞ҐႨ200Lđ࢞၁ุࠒູ150Lđ◆1■2▲3×4*5●6789ٳљҐࠢ࢞ު6a8a10a1