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1、棉花学报CottonScience2013袁25渊5冤院426~431盐分胁迫下2-氯-6-(三氯甲基)吡啶浸种对棉种发芽及其生理特性的影响*陶瑞袁刘涛袁褚贵新渊石河子大学农学院/新疆生产建设兵团绿洲生态农业重点实验室袁石河子832003冤摘要院通过设置0尧80尧120mmol窑L-13种NaCl浓度袁研究了3种盐分水平下2-氯-6-(三氯甲基)吡啶浸种对棉种发芽的影响袁分析了棉种萌发过程中可溶性蛋白尧可溶性糖尧抗氧化物酶及丙二醛含量对2-氯-6-(三氯甲基)吡啶浸种的生理响应遥结果表明袁盐分胁迫显著抑制了棉种的
2、萌发袁而2-氯-6-(三氯甲基)吡啶浸种能明显减轻盐分胁迫对种子发芽的抑制遥在3个盐分水平(0尧80尧120mmol窑L-1)下袁2-氯-6-(三氯甲基)吡啶浸种处理的发芽率尧发芽势及种子活力指数均比清水浸种有显著提高袁其增幅分别为11.5%～58.4%尧16.8%～65.0%和42.9%～82.5%曰同时袁2-氯-6-(三氯甲基)吡啶浸种可显著提高棉种SOD尧POD尧CAT的活性袁分别比清水浸种增加了57.2%～282.7%尧8.3%～139.3%和6.4%～15.1%袁且显著降低了电解质外渗率与MDA含量遥在
3、盐分胁迫下袁利用4.1伊10-3mmol窑L-1的2-氯-6-(三氯甲基)吡啶浸种可提高棉种抗氧化物酶活性袁促进其萌发过程中可溶性有机物的转化袁显著提高棉种发芽率和发芽势遥关键词院2-氯-6-(三氯甲基)吡啶曰盐分胁迫曰棉种曰发芽率曰酶活性中图分类号院Q945.34文献标志码院A文章编号院1002-7807(2013)05-0426-06*TAORui,LIUTao,CHUGui-Xin(832003,)Astimulationexperimentwascarriedouttoinvestigatetheeffe
4、ctofnitrapyrinsoakingofcottonseedoncottongerminationrateunderdifferentsaltstressconditions(0,80,120mmol窑L-1NaCl).Meanwhile,cottonseedactivitiesofantioxidaseandcontentsofseedwater-solublesugar,water-solubleprotein,antioxidantenzymesandmalondialdehyde(MDA)incot
5、tonseedweremeasuredduringthecottonseedgerminationprocess.Anobviousinhibitioneffectoncottonseedgerminationwasobservedundersaltstress;butnitrapyrinsoakingtreatmentssignificantlyincreasedcottonseedgerminationrates,germinationpotential,andcottonseedvigorindex.Com
6、paredwithwatersoakingtreatment,germinationrateincreasedby11.5%～58.4%,germinationpotentialincreasedby16.8%～65.0%andcottonseedvigorindexincreasedby42.9%～82.5%whenusingnitrapyrinsoakingtreatmentatthesamesaltconcentrationlevel,indicatingnitrapyrinsoakingplaysasig
7、nificantroleonalleviatingthesaltinhibitioneffectofcottonseedgermination.Nitrapyrinsoakingtreatmentsstatisticallyincreasedcottonseedenzymeactivities.Comparedwithwatersoakingtreatment,enzymeactivitiesofSOD,POD,CATincreasedby57.2%～282.7%,8.3%～139.3%and6.4%～15.1%
8、withnitrapyrinsoakingtreatmentsatthesamesaltconcentrationlevel,respectively.Furthermore,cottonseedelectrolyteleakagerateandMDAcontentofnitrapyrinsoakingwerelowerthanthatofthewatersoakingt
