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1、棉花学报CottonScience2015袁27渊4冤院300―309光质对离体培养条件下绿色棉纤维发育及黄酮合成的影响111,2111*11洪亮袁张雷袁钱森和袁孙旭袁蔡永萍袁林毅袁高俊山袁郭宁渊1.安徽农业大学生命科学学院袁合肥230036曰2.安徽工程大学生化学院袁芜湖241000冤摘要院为探讨光质对绿色棉纤维生长发育和黄酮合成的影响袁以绿色棉品种绿絮棉1号为材料进行离体培养袁比较红光尧黄光尧蓝紫光尧白光和暗处理条件下绿色棉胚珠鲜物质质量尧纤维长度尧纤维素含量尧黄酮含量等生理和分子指标的差异遥结果表明院光处理能够促进胚珠鲜物质质量的增加
2、袁但抑制了纤维的伸长遥在纤维发育前期袁各光处理抑制了蔗糖合成酶活性和蔗糖合成酶基因表达曰纤维发育后期袁白光处理提高了蔗糖合成酶活性袁促进了蔗糖合成酶基因表达遥红光尧黄光和蓝紫光处理显著提高了茁-1,3轧葡聚糖酶活性渊10～20DAC渊Daysafterculture冤冤曰红光尧黄光和白光均能提高茁-1,3轧葡聚糖基因表达量袁而蓝光则抑制了基因的表达遥白光促进了纤维素的合成袁而蓝紫光抑制了纤维素的合成遥在纤维发育初期渊10DAC冤袁蓝紫光抑制了黄酮合成及其关键酶基因表达曰而在发育后期渊30～40DAC冤袁白光处理能够促进黄酮的合成及其关键酶基
3、因表达遥可见袁光质对绿色棉纤维发育及黄酮合成具有一定的影响遥关键词院绿色棉曰离体培养曰光质曰基因表达曰纤维发育曰黄酮合成中图分类号院S562.03文献标志码院A文章编号院1002-7807(2015冤04-0300-1010.11963/issn.1002-7807.201504002111,211111HongLiang,ZhangLei,QianSenhe,SunXu,CaiYongping,LinYi,GaoJunshan,GuoNing渊1.230036,;2.241000,)Toinvestigatetheeffectofligh
4、tqualityongreencottonfiberdevelopmentandflavonoidsynthesisinthegreencottonvarietyL俟xumianI,physiologicalandmolecularindicatorsweremeasuredasamaterials,andtheovulefreshweight,fiberlength,cellulosecontent,flavonoidscontentandotherphysiologicalandmolecularindicatorswithredlig
5、ht,yellowlight,bluelight,whitelight,anddarkgrowthconditions.Alllightconditionstestedpromoteovulefreshweight,whileinhibitingfiberelongation.Duringearlyfiberdevelopment,alllightconditionsinhibitsucrosesynthaseactivityandsucrosesynthasegeneexpression.Onlywhitelightimprovessuc
6、rosesynthaseactivityandpromotesexpressionatalaterstageinfiberdevelopment.Red,yellow,andbluelightsignificantlyincreased茁-1,3-glucanaseactivity(10-20Dayafterculture,DAC),whilered,yellow,andwhitelightimproved茁-1,3-glucanasegeneexpression.Onlybluelightsuppressedexpressionatthi
7、slocus.Cellulosesynthesisispromotedbywhitelightandinhibitedbybluelight.Duringearlyfiberdevelopment(10DAC),bluelightinhibitsflavonoidsynthesisandexpressionofkeygenesinthispathway.Duringlatefiberdevelopment(30-40DAC),whitelightpromotesflavonoidsynthesisandkeygeneexpression.T
8、hus,thesefindingsuggestlightqualityhasacertaininfluencesonthefiberdevelopmentandflavonoid
