不同牧草缓冲容量的比较研究本科毕业论文定稿

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1、仲恺农业工程学院毕业论文不同牧草缓冲容量的比较研究姓名院（系）生命科学院专业班级学号指导教师职称教授论文答辩日期2013年6月10日仲恺农业工程学院教务处制承诺书我承诺在毕业论文活动中遵守学校有关规定，恪守学术规范，本人毕业论文内容除特别注明和引用外，均为本人观点，不存在剽窃、抄袭他人学术成果、伪造、篡改实验数据的情况，如果有违规行为，我愿意承担一切责任，接受学校的处理。学生(签名)：2013年6月10日摘要禾本科植物和豆科植物有不同的缓冲容量。禾本科植物的缓冲容量低(P>0.05)，豆科植物的缓冲容量比较高（

2、P>0.05），通过本实验计算得到狗牙根的缓冲容量为0.55，柱花草的缓冲容量为1.51，象草的缓冲容量为0.91，缓冲容量越高，则牧草在青贮过程中需要形成的乳酸越多，如豆科植物，因其缓冲容量高，不添加添加剂就很难青贮。缓冲容量的高低与植物体内粗蛋白含量和可溶性糖含量相关,缓冲容量高(P>0.05)的牧草，其粗蛋白含量和可溶性含量也高（P>0.05）。关键词：牧草青贮pH值缓冲容量目录1前言····························································1

3、2材料与方法······················································22.1试材及试样准备·················································22.1.1象草的采集和烘干·············································22.1.2柱花草的采集和烘干···········································32.1.3狗牙根的采集和烘干······

4、·····································32.2测定方法和步骤················································32.2.1牧草缓冲容量的测定···········································42.2.2牧草粗蛋白的测定·············································42.2.3牧草可溶性糖含量的测定····························

5、···········53.结果·····························································53.1不同草本科植物的缓冲容量·······································53.2不同草本科植物粗蛋白含量·······································63.3不同牧草可溶性糖含量···········································74.讨论·········

6、····················································8参考文献···························································9英文摘要···························································10致谢······························································111前言牧草青贮过程中

7、，参与活动的微生物种类很多,而以乳酸菌为主,所以青贮的关键在于乳酸发酵的程度。正常青贮时,各类乳酸菌在含有适量水分、碳水化合物以及缺氧环境下,快速繁殖,可生成大量乳酸,少量醋酸、丙酸、琥珀酸等。牧草通过乳酸菌发酵而达到一定的酸度(一般为pH4一4.2)，这是青贮成功的前提条件。大多数牧草的pH值大约为6左右.为了达至稳定青贮的目的，乳酸菌发酵形成的酸不仅要中和牧草原有的碱性，而且牧草本身还具有缓冲pH值变化的能力，也需要一定数量的酸。牧草这种缓冲pH值变化的能力越强，则需要形成的乳酸越多.同时[1]也就需要更多

8、的可发酵碳水化合物。牧草这种对pH值下降的自然缓冲能力，就称之为牧草的缓冲容量。牧草的缓冲容量就是pH值达到4.0时所需的乳酸数量，其单位为摩尔(乳酸)/kg或100g牧草(干重或鲜重)，或g(乳酸)/kg或100g牧草(干重或鲜重)。本实验采用的牧草有禾本科象草、狗牙根，豆科植物柱花草。象草象草是南方地区的主要栽培牧草,具有生长快、产量高、适口性好等优点。象草是我国南方建立人工草场的