微生物检验主管技师基础知识模拟1

《微生物检验主管技师基础知识模拟1》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。

ᱥᢈẠ11.Ḅᑨ“”"#Aஹ%&'ᐰ)*+,-ᕈ/012Bஹ%&34'ᐰ)*-ᨵ/012Cஹ%&6)*-ᨵ/012Dஹ7+ᨵ89)*:-ᨵᶍ12Eஹ7+<ᙳ>?-@12AᫀCE2.DEFG⊈&ᨬJKLAஹ4࠷BஹMNMOPDECஹ-20࠷DஹRSTUDEEஹ40࠷DEAᫀCD3.VWXMNḄY:Aஹ4࠷Bஹ4࠷—(-18)࠷Cஹ0࠷Dஹ-10࠷Eஹ-2(TCZ[\]AᫀCB4.VW^YMNḄY:Aஹ4࠷

1Bஹ4-8࠷Cஹ0࠷Dஹ-10࠷Eஹ-20࠷Z[\]AᫀCE5._ᩩaLbcd₩ᘤḄghAஹᶖk⌱mn+oBஹmnY:pឤஹ<ᙳCஹᵨ,s+mnmntDஹtuvwஹxyzEஹ7+mnmnt\]AᫀCC6.{DE|}ᵨḄ~ᜓAஹTUNBஹSTCஹDஹᵯEஹᑖᐝᐝ:\]AᫀCB7.■ᡈᢓᑴt
uḄ■AஹBஹ■ῖCஹᢓDஹ◀Eஹ\]AᫀCB

28.{Ḅ¡}¢ᵨ⌕¤ᑮᡂᦔ¨᰿ªᘤ'«,u¢ᵨ®¯¢ᵨᨬ°⌕Ḅ±²³⚗AஹTUBஹµ¶

3Aஹ<åæBஹØ<åæCஹØ<åØDஹæ<åæEஹæ<åØAᫀCC12.ᵨçè⏣12᪶⏚ÞᵨÍ⏣12ëìᨬWíḄghLAஹᢵïwBஹãðÓÏ7+oCஹãñᜧÏ7+oDஹó᧕õ◀Eஹⅅ÷ᡈøᓄúû᧕ÞDNAᐳ12AᫀCB13.ᵨÍ⏣12Ï,SDSḄRNAýþF¢ᵨḄÿ
Aஹ⏚Bஹ⏚Cஹ⏚Dஹᓄ┞EஹᓄᫀC14.ᑴᜓᡠᵨ⏘
ᵨ☢!"ᑺ$ᑴḄAஹTEBஹTAECஹTBEDஹTris-HCl

4Eஹ&⏚ᫀD15.'ᦿ)*ᵨ+,-./Ḅ!0"ᱯឋAஹ./Ḅ᪶⏚"Bஹ./Ḅ456Cஹ./Ḅ7Dஹ./Ḅ89:6Eஹ./Ḅ;▬6ᫀB16.=>☩ᔆ0ABᒑD0EFGḄ>HIJKᐜMNDOPIQRP⚔TMNUVIᑁXYZ⁐\Iᕜ^_`aMbcIdefg◍IiHjklmNm⊈ឋoFIpᕜᨵᡂsḄtUV஺vmVḄᑖxᱥzᱏ|}☩ᐲ5⁐5⁐஺ᨵḄ☩ᐲ□ឋ⁚ᜧᩚ஺ᢥ᯿ᑖ᪆IAB
45DAஹ∑᪵ᵯᩚBஹ₝ᩚCஹᩚDஹ¡ᾦ£ᩚEஹ¤¥ᩚᫀE17.)¦§¨,©ªIᢙ\Ḅ□ឋ᪗®Aஹ2140BஹN180CஹN1160Dஹ21320EஹN1640

5ᫀD18.¯᪶°)*,72hH¯±ᑨ-®³□ឋ´µIᐸ·Tbᜧ¸¹ᶖ®AஹW2mmBஹ3—4mmCஹ5—9mmDஹll-19mmEஹN20mmᫀE19.Ὂ½¾¿ᙠX8%NaCI▰UjÂÃḄÄ
AஹÅ᝱ÄBஹÇÈÄCஹÉÄDஹÊËÄEஹÌ⊈ÄᫀE20.ᢙÍ“O”)*:ÎÏÐÑÒAஹ¯᪶.BஹÓÔCஹÕᾯDஹ×RØEஹញឋÚtᳫÚÔᫀE21.ܨ.B⊈᪗ÝÞßḄà⚗
Aஹµᙠ.Bâᵨᢙãᱥäåܨ

6Bஹæ0ç⊈᪗ݵæè◤êëêìíCஹëêìíᨵîÂÃIïÂᓄ´µðµᙠëêñ᛻j|}஺Dஹóᶧ®õö¡÷.BḄ⊈᪗ݵøìíᑮú4ᕜEஹûḆ
ᫀE22.⊈ýᾦþÿ
AஹẠBஹ␺Cஹ⃩Dஹ⌱EஹᫀC23.ᜧḄᓫAஹmmBஹpmCஹnm!Dஹ"ngEஹ⍝$%DaltonᫀB24.&
'()*⌕ᡂᑖḄAஹ᪶/01Bஹ(2Cஹ᪶(Dஹ34Eஹ'5⚩2ᫀD

725.789ᳫᙠ<=>?@,BCDEF⁐AஹH⁐Bஹ⋎⁐CஹJ⁐DஹK⁐EஹLM⁐ᫀC26.ᾯO8᜼Q
Aஹ☩ᐲT□ឋᳫBஹ☩ᐲT▢ឋᳫCஹᨵYᱥDஹ☩ᐲT□ឋᩚEஹ☩ᐲT▢ឋᩚᫀA27.\]⊈_(`abcḄd&e⚗g&ᒹiAஹcjE⊈_)ᱯlᢙ1ḄnBஹ⊈_᜜pCஹ⊈_ᱯlᦔrDஹ⊈_ᔾtuḄvwxyEஹ⊈_Ḅzb{ᫀA28.|tᢙ}~ẚ'ᵨdḄAஹ⏘ᜐᳮBஹCஹẚ

8DஹᵨSDSᜐᳮEஹᓝᜐᳮᫀB29.&〉ᔠ⍝ᑖ␺cᵨḄAஹấ⏚ᾦBஹH¡ᐲCஹ¢£¤ᾦDஹSS<ᾦEஹ¥ឋ/0▰§ᫀA30.¨©?ᱥªᱯឋḄ␺cᨬ¬⌱®?@¯Aஹ°±¯Bஹ²ᦪ¯Cஹ´µ¯Dஹ⊦·¯Eஹ¸®¯¹º»ᫀB31.¼½?@¾¿ḄᩩÁ~&ÂᑗḄ<ᩩAஹ?@¾¿◤⌕ÅÆᐙÈḄ⃩ɺᩭËBஹ◀ÍὊ⏚¥ḄÏ᜜,²ᜧeᦪᔠ〉ḄpHpH7.2-7.6Cஹ35஺2—37ᨬ〉ᔠᡠᨵ?@DஹᨵÒ?@◤⌕,Ö×ឋᜧØÙTÚBC

9Aஹ᪻H⁐BஹÜH⁐CஹM⁐Dஹz⁐EஹÜᮞ⁐ᫀA33.Þߧஹàᛢ§â᜜ã᛻§)å§᪵¨©çèéᑖê~<᪵åëAஹ<ìíᑁḄ⊤ð§Bஹ)ð§CஹñòóḄ§Dஹᔜð§ᙳöEஹòó÷øᫀA34.gùᡃûüýþᵨḄÿ
AஹTy21aBஹᐰ
Cஹ
DஹVi
Eஹὶ
ᫀD35.ᣚ᪆ᵨAஹBஹ᝘Cஹ!"Dஹᵞ$

10Eஹ%&'ᫀC36.ᣚ᪆ᢈ)⌕+,ᵨAஹᑖ./ᓄ12Bஹ3ḄᑖCஹ'ḄᑖDஹ᪶⏚Ḅᑖ./ᓄEஹ7Ḅ8ᓄᫀA37.9ឋ;<Ḅ=>Aஹ?@BஹABCCஹẚEFGDஹHᳫEஹJKLᩚᫀC38.N01OḄPQRSTAஹUᐺW01OPQRBஹ0139OPQRCஹXYRDஹUXYREஹᐸ[\ᫀD39.ᑴᜓ_ᵯaᯖᵯcXdUePHḄf⌕ᱥAஹhᾦ'

11BஹjCஹkឋᵯlDஹ<ᓄmEஹᵞn⏚opᫀC40._ᵯaᯖᵯcqᳮAஹXdᔾ▬uᵨBஹXdvwuᵨCஹXdxᡂz{|}~᪀DஹXdḄ7ឋEஹkឋᵯlᑴ⌼XdUeḄPHUeᵯ₹Ḅzᙠᵯcᩩ_ᵯᶇopᫀE41.AmpholineᵨAஹhᾦBஹa⏒XdᵯcCஹᾭᵯXdᵯcDஹ_ᵯaᯖXdᵯcEஹᚖᵯcopᫀD42.IFE>Aஹhᾦ'ᵯcBஹa⏒XdᵯcCஹᾭᵯXdᵯcDஹᚖᵯcEஹ_ᵯaᯖᵯc

12opᫀE43.-ᵨ⌶AஹᑖḄ/ᓄ12Bஹ3ḄᑖCஹ'ḄᑖDஹ᪶⏚Ḅ/ᓄEஹ7Ḅ8ᓄopᫀD44.ᑴᜓ=Ḅ᜜12¡ᵨ¢£¤¥ᜓAஹ¦§¨Bஹ¡©ᐝᑖᐝᐝ«¬Cஹ᜜ᑖᐝᐝ«¬Dஹ®¯°=±²³Eஹ®¯°´µᘤopᫀD45.hᾦ'XdᵯcḄᵨ⌶Aஹᑖ./ᓄ12Bஹ3ḄᑖCஹ'ḄᑖDஹ7Ḅ8ᓄEஹ᪶⏚Ḅᑖ./ᓄopᫀE46.ᾭᵯcᵨ⌶Aஹ3ḄᑖBஹ᪶⏚Ḅᑖ

13Cஹᢙq¸¹º¸Dஹ12ḄᑖEஹ7Ḅ8ᓄopᫀB47.ᵨ⏒⌕9K»¼½¾■ÀÁÂAஹ⏒ᢵÄBஹ⏒᧕ÆCஹ⏒ÇÈDஹ⏒ᐹÊËEஹ⏒᧕ᰦÍopᫀD48.SDS-a⏒Xdᵯcᑖᩩ,ÏAஹÐᑖᱥBஹᜧᑖᱥCஹÒᑖᱥDஹÓᔠEஹÐÒᑖᱥopᫀC49.ℍ⃔ᳫ¡ᑖ×Ø⁐ℍ⃔ᳫÁ⊤Ûℍ⃔ᳫஹῖÝℍ⃔ᳫÁᐸÞßḄᑖàá⌕+>ᢥ᯿ä¤ᱯæAஹXç⏘Bஹ⁐CஹÄ⏗&'DஹÄ⏗ᵞ☄⏣EஹᑁopᫀA

1450.SDS-a⏒Xdᵯcᑖᩩ,íÏAஹÐᑖᱥBஹᜧᑖᱥCஹᑖᱥDஹÓᔠEஹÐÒᑖᱥopᫀB51.SDS-a⏒Xdᵯcîïf⌕ᡂᑖAஹᓄmBஹᵞn⏚Cஹᵞ$Dஹðñ⏚Eஹ⏒opᫀB52.ᓝóôõñ⏚ma⏒XdᵯcÁᵨḄ12öᧇAஹὃùúûᐲBஹýᐲCஹþᐲDஹÿᐲEஹ✏₝ᐲᫀA53.ᓝ⏚⏒ᵯᑖ!᪗#$%ᵨ⌶()*ᡃ,-.Ḅ$%ᑖ!MarkAஹ01ᵯᩩ3ᑖ!Bஹ45$%6

15Cஹ78ᵯDஹ9ᔠ;ᙳEஹ=ᓄᔠᫀA54.ᜧ@ᦪ▲ᑴឋᑁᑗ⏘ḄHᵨIJAஹ10࠷Bஹ20࠷Cஹ30࠷Dஹ37࠷Eஹ50࠷ᫀD55.ᜧ@ᦪ▲ᑴឋᑁᑗ⏘ḄLMIJAஹ4࠷Bஹ4-8࠷Cஹ0࠷Dஹ-10࠷Eஹ-20࠷ᫀE56.PCRNOPᑡRSᱥ6U*V◤ḄXYAஹZᱥBஹDNAᔠ⏘CஹMg2+Dஹ[ᱥ\EஹdNTPᫀD

1657._`Ḅab*ᢣᩔefg◀᜜j᛻ḄlmnoᱥᭆqḄ]^*AஹVrᩔstᨵvwḄxyBஹU⌕{ᩔeᡈg◀ᡠᨵḄnoᱥ~vwCஹᩔe᜜j᛻ᡠᨵnoᱥDஹᩔsᡈg◀lmnoᱥEஹg◀ᡠᨵḄnoᱥᫀB58.Ixyᐹᨵsᵨ-ᵨ_`fey@ᦪvwḄxyᙠ55-60IJPᵨ@sAஹ5-10ᑖBஹ15-20ᑖCஹ30-60ᑖDஹ1-2Eஹ2-4ᫀC59.ᙠᩩPᨬ◤⌕@IJᵨ5-10ᑖᩔsᡠᨵxyf⏗yAஹ40࠷Bஹ50࠷Cஹ60࠷Dஹ70࠷Eஹ80࠷ᫀE60.¡¢8£␺aḄxy*Aஹ¥¦§ᳫy

17Bஹ©ª«⊈ᩚyCஹᾯ¦ᳫyDஹ¯ᳫyEஹᓱ±ᳫyᫀA61.ᙠ²³´µḄ¶·¸oᕈº»ᡈ᪷r½¾H´µ¿9ÀḄxyAஹÁÂBஹoÃÄCஹÅÆDஹᨵÁÂEஹÇᨵᩖyᫀD62.ᑴᜓ$%ᢙmËJÌÍUᜫÏḄÐÑ*AஹÒfÓ᪆ÑBஹ5ÕᣚÓ᪆ÑCஹÓ᪆ÑDஹ×£5ØÑEஹ⏚ÙÚaÑᫀA63.@ÛܲᢙmÝÞᑮàáâ*ᳮäḄåᔠAஹ10ÜBஹ20ÜáCஹ5ÜDஹ12ÜEஹ15Ü

18ᫀB64.ᙠæçè´µáRéSxy⊤ëìíAஹîïᩚyðñòᩚyBஹᜧóᩚyðñòᩚyCஹᜧóᩚyðîïᩚyDஹôõ⊈öyðñòᩚyEஹôõ⊈öyðîïᩚyᫀA65.ᵨ÷øöyḄùúLM´µûAஹüឋ$%▰þBஹP.V;ÿCஹSS
ᾦDஹECᾙEஹGNᫀA66.ᑡḄ⌱ឋ⌱ឋḄᨵAஹឋ
ᾦBஹឋ
ᾦCஹSS
ᾦDஹ4!
ᾦEஹ"#$
ᾦᫀD67.%ᱥ'ᐰ)⌕+,ᳮAஹ./Bஹ0123ᘤ◀67ᔣ9:

19Cஹ■Eஹ017ᔣ9:ᫀB68.?@ᩭBᑖD%ᱥEFGHIJᵨLMᵯOBᵨPᑖQDNAᜧᱏTUVWPAGEXLMᵯOAஹYZ[⏒]^_ᵯOBஹ`aᵯOCஹb9cdᵯODஹef7gcdᵯOEஹh⏚jklᵯOᫀA69.Qmnᢥ᯿qᜮstuᑖvwMAஹ1MBஹ2MCஹ3MDஹ4MEஹ5MᫀB70.xLMyz{|ᓣ~ᙳ᠒Byᑁz{ᙳAஹyBஹ◞yCஹᵯឤz./ᵨyDஹ₩yEஹᵯᵨyᫀB

2071.⌕+ᵫᑡMAஹᡚBஹCஹDஹ¡¢Eஹ£᪛¥ᫀB72.¦§9¦⊈ᩚḄª«¬ὅBᐸ°±²=IAஹ³⊦µBஹᾯlCஹῳ¸Dஹ¹º»Eஹᾱ½ᫀB73.¾¿À⊤ÂÃÄÅḄ±ÄÆuÇvAஹBஹÈ™ÊCஹᡚDஹEஹ¡¢ᫀB

2174.¾¿À⊤ÂÃÄ⌕+vAஹÄBஹ±ÄCஹ᜜¦§ÄDஹ³⊦ÄEஹ³Ì⊈ÄᫀA75.¦§Ḅ¾ᮤÎÏ⌕+ᙠAஹ1ÑÒlBஹÓᗕÎCஹÕDஹÖEஹ×1ÑÒlᫀC76.¿À|Øᶭ¾ḄIᵨÚ┦ÜᱥvAஹBᑁ⏒]ÃBஹÝÞßÃCஹᜧàᑁáÃDஹâ]ÃEஹãäåæÃᫀC77.ç᪶ᑖ×ᩚéêë,IᵨḄíîᱏïðvAஹ☩ᐲó§⁐Bஹᢙ⏚§⁐Cஹö÷⏚-ùú§⁐Dஹ₯ᐝ§⁐

22Eஹý§⁐ᫀB78.ᡠᑴᜓ
Ḅ,◤⌕᪵ᙠ1.05kg/cni2Aஹ10ᑖBஹ15-20ᑖCஹ30-40ᑖDஹ30ᑖEஹ60ᑖᫀB79.Ớ⏀ᵨ"#ᑺ%&'〉ᵨ)*"#Aஹ1%Bஹ2-2.5%Cஹ3-4%Dஹ4-5%Eஹ5-6%ᫀB80.+,᪗./012ᜐ4ᐹᨵ789:Aஹ;᪶ᑖ=ᩚBஹᾯ@A᜼CCஹDAEᳫDஹGH⁐ℍ⃔ᳫEஹL⊈ឋOᳫᫀA81.PQRS☠ᢙV

23AஹBSABஹ4᜜#XCஹSRBCDDAᳫY@ZEஹᐢ⊈\]^]ᫀD82._`abcadᕜᑁ,ᑖgaVhijḄ᪗.AஹᕏᔢᱥBஹnoCஹ⊈pDஹᾯqpEஹrpᫀC83.ᙠᾯ@Aabᾯqp/stuvḄwAஹᾯ@AᳫBஹDAOᳫCஹ;᪶ᑖ=ᩚDஹᵭyz{|Eஹ}~◚ᳫᫀE84.0u/rᨵ9:Ḅ4ᦪAஹ☩ᐲ▢ឋᩚᦪ
104/mlஹ☩ᐲ□ឋᩚᦪ
103/mlBஹ☩ᐲ▢ឋᩚᦪ
103/mlஹ☩ᐲ□ឋᩚᦪ
103/mlCஹ☩ᐲ▢ឋᩚᦪ
105/mlஹ☩ᐲ□ឋᩚᦪ
104/mlDஹ☩ᐲ▢ឋᩚᦪ
102/mlஹ☩ᐲ□ឋᩚᦪ
103/mlEஹ☩ᐲ▢ឋᩚᦪ
103/mlஹ☩ᐲ□ឋᩚᦪ
102/ml

24ᫀc85.ᘤ᣸hḄᱥAஹᢙVBஹᢙVCஹDஹឋᢙVEஹ~ᢙVᫀE86.s#⊈Ḅ¡AஹaL⊈OᳫBஹῖ£ℍ⃔ᳫCஹẚ_¥¦Dஹ§_`¨©EஹGH⁐ℍ⃔ᳫᫀC87.ª«ᜧᑺᢙV®ᡂ°±Ὂ³´µAஹᢙ¶®ᡂ·&᠒¹º»·&¼½BஹᢙV¼ᒕ¿᜜ḄᢙV/ÀÁᢙ¶CஹB4ÂᑖᓄÄÅƳᑮᢓᑴDஹÊ£ËÌsḄᢙ¶EஹÍ4ÂÎÏᫀC88.ᑖÐ~IgAᨵÑÒᢙ^]ÓÔ⏘ÖᵨḄ×ᦑAஹJOÖᵨBஹᑖÐᱏ;ᔠᙠÛOÜᕜÝ

25CஹᑖÐᱏᢙ⏘ÖᵨDஹIgAḄFCÖᵨEஹ2ÞIgA;ᔠÖᵨᫀB89.ᵨQRPS☠ឋᢙV°±⌕ßàáÊ£Ḅ°±ᳫ^]AஹIgGBஹIgMCஹIgADஹIgEEஹIgDᫀB90.ᦪ¨©ᙠyEZâKIAãᔠäå/ḄhAஹæ☢èéஹêëèHBஹæ☢ÀêëᙳèHCஹæ☢ÀêëᙳèéDஹæ☢èéஹêëèíEஹæ☢èéஹêëËèᓄᫀD91.îï¼ð¼ñ/ᐜóῃḄAஹ/ᑖ{ᱥBஹᜧᑖ{ᱥCஹõᑖ{ᱥDஹöᔠEஹ/õᑖ{ᱥᫀB

2692.îï¼ð÷⌕ßᵨ⌶Aஹ^]ḄᑖgBஹùÓᓄÀᱥḄᑖgCஹᾦûḄᑖgDஹ^]ᡈ᪶⏚ḄᑖgEஹÓḄþᓄᫀD93.îï¼ð÷ÿ
AஹᑖBஹᑖ᪆CஹDஹ᪆EஹᫀB94.ᨬᵨḄAஹBஹ᝘CஹℍDஹᵞ"Eஹ#$ᫀC95.Sephandex%Aஹ&ᾦBஹⅅCஹℍDஹ)*᧖

27Eஹ#$ᫀC96.,-./ῃḄ1Aஹ-ᑖᱥBஹᜧᑖᱥCஹ4ᑖᱥDஹ5ᔠEஹ-4ᑖᱥᫀC97.7ᳮ1Aஹᔾ▬;ᵨBஹ<=;ᵨCஹ>ᵯ₹ABDஹCᡂEFGHI᪀EஹḄKLឋᫀD98.ᵨNO᪆ḄOPAஹQ⏚SBஹQ⏚╅CஹU⏚VDஹWᓄYEஹWᓄ┛ᫀC99.O᪆⌕\ᵨ⌶Aஹ^_Ḅᑖ`aᓄ

28BஹOḄᑖCஹḄᑖDஹ᪶⏚Ḅᑖ`aᓄEஹLḄcᓄᫀA100.defgᡈfgᢙ7jkᙠmὅopEqrst᪵vwᑮyᐸ-Aᒹ|}ᑡqAஹᱏBஹᾯCஹ⊈DஹEஹᜐᫀCᱥᢈᳮ8A11.ᐲ¡¢⁐1¤¥¦-§¨⌕␺ᑮ¢⁐yᑨ«I¬1AஹG-¥
⁐,G+
¯⁐BஹG-¥
¯⁐,G+
⁐CஹG-¥
°⁐,G+
⁐DஹG-¥
⁐,G+
°⁐EஹG-¥
±⁐,G+
¯⁐ᫀB2.²¤¥ḄCHI᪀³Ḅv´1µ¶·¸¹yᙠ¸¹ᐸº»¼◤⌕Aஹ¾ᐲ¢⁐Bஹ☩ᐲ¡¢⁐Cஹᢙ⏚¢⁐DஹA¢⁐EஹÀ¯¢⁐

29ᫀD3.}ᑡᐵNÂ*pḄÃÄyÅÆḄ1Aஹ☩ᐲ¡¢⁐□ឋBஹ☩ᐲ¡¢⁐▢ឋCஹ²☘◭ËÌ┦Dஹ²¯◭ËᢙÎEஹឋ◤ÏᫀA4.}ᑡᐵNÐÑÂ*pḄÃÄÅÆḄ1AஹᨵÓÔBஹ╄S¢⁐yÐp
¯⁐Cஹ╄S¢⁐,Ðp
Ö⋎⁐Dஹ☩ᐲ¡¢⁐□ឋEஹØ1ÙÚÛᫀC5.ᙠÜÝCFTÞß,⌕²àv⊈áÜÝâã⊡pyåABºᱥ⊈áḄ⊡pâãæç1Aஹ65࠷Bஹ56࠷Cஹ37࠷DஹèBḄEஹABḄᫀE6.éÝឋᦿëìḄíîïAஹð

30Bஹ∭CஹòDஹ⇟EஹôᫀD7.ឫöfḄíîïAஹðBஹ÷CஹòDஹ⇟EஹôᫀE8.ᙢùឋᦿëìḄ⚜■üAஹâýஹâ⇟
þ⌕ÿBஹஹ⇟⌕Cஹஹ⌕ឋDஹ⌕Eஹ∫ஹ⌕ᫀC9.ᜩᑡḄ!ᵨ#$%ḄAஹᜩ&'Bஹ(Cஹ)&'Dஹ*'(Eஹ+,-.ᫀC

3110./0ឋ123ᨬᐹᨵ/0&789Ḅ+:;ᑡAஹ<=>?ὅBஹA&BCCஹA&DCDஹA&ECEஹA&FGCᫀA11.ᙠI/ᵯKLM᪵OPQRSAஹT⏚VQRSBஹTris-HClQRSCஹWXYQRSDஹZ⏚V-Z⏚QRSEஹ\⏚VQRS]^ᫀC12.ᑡ_⌱⚗bᑁDNAFᑴeb᜜PCRg!hiḄAஹjkᶇFᑴBஹdNTPmGᧇCஹoᔣFᑴDஹqr⏘teEஹDNAuᔠ⏘te]^ᫀD13.ᑡw&xLhᐹᨵyz{ஹhyᐭ}~-.ḄAஹxBஹxCஹAᩚx

32Dஹ}}3ὤxEஹx]^ᫀB14.xᢙGFᩖ,ᑡh⌕Ḅᐹᨵᑖ789ḄᢙGAஹOᢙGBஹHᢙGCஹKᢙGDஹFᢙGEஹViᢙG]^ᫀD15.-x,.ᑁxḄAஹETECBஹEPECCஹxDஹxEஹx]^ᫀD16.ᑡᐵ3¡ᩚxḄ¢£]^ḄAஹ☩ᐲ:⁐□ឋBஹᐹᨵ¨©᪀Cஹឋ◤¬Dஹឋ¬Eஹ⃩¯◤°±]^ᫀB

3317.²:ᑡ-x³M´µ¶ᱞ¸¹{ḄAஹETECBஹEPECCஹxDஹxEஹx]^ᫀD18.ᑡᐵº»⁐ℍ⃔ᳫxḄ¢£]^ḄAஹ☩ᐲ:⁐▢ឋBஹÀÁ¸⏘Cஹhr⏗ᵞ☄⏣Dஹǯ◤⌕ǯÈEஹÉ⌕ÊxËÌ]^ᫀB19.ᑡÍ£]^ḄAஹ᫥'ÏÐbÑÏÐbBஹ᫥'ÏÐbÒÓÔ¡ÕÏÐbCஹ᫥'ÏÐbÖ×ǯØ᧕Dஹ᫥'ÏÐbᢙÚ{ÛEஹ᫥'ÏÐbᐹᨵᑁ'Ü]^ᫀB20.ᑡÍ£]^ḄAஹÏÐbÝÀw&BஹÕÏÐbÝ´w&CஹÏÐbLÞᨵÕÏÐb´w&DஹÕÏÐbᒹàw&áâw&ãᜧ

34EஹÕÏÐbLÞᨵ᫥'ÏÐb´w&]^ᫀD21.åæÏÐb²:´r»çèéMᐸëᦑᵫAஹ-.'îïðᵨBஹñ⊈ÜðᵨCஹᾦôõDஹ᜜©ö¡Eஹ÷øᫀB22.EPECI-.Ḅù▬ᨵAஹûᦣឋù▬Bஹý▲ឋù▬CஹAஹBÿ
DஹAஹBEஹᱯឋ▬ᫀC23.ᑡḄAஹ⊟ᜩᯠᱥ!Bஹ⊟"#$%&Cஹ⊟'()ឋ*Dஹ⊟+,-᧕Eஹ⊟/0123ᫀBAஹ4ᾦ᡽ᦣ8942:;

35Cஹ⊈?8@AB89DஹCCFTEஹDEFGᑡᔜIJKḄLMᑖO24.24PLᫀC25.30ᑖᫀB26.3-7ᑖᫀE27.72PLᫀA28.ᑡQIR#ᱥᐹᨵUᾱWAஹXYZBஹ[UᎷᓫ^Cஹ_`aᩚZDஹcde⊈ᩚZEஹfᳫZᫀB29.ᡃij#᪗lmn00mlopqrstumḄᜧwZxᦪz{|}Aஹ1Bஹ3Cஹ5Dஹ7Eஹ9ᫀC

3630.ᑡQI~Zឋ◤ZAஹXYZBஹ_`aᩚZCஹᜧwᩚZDஹUᎷᓫ^ZEஹᫀD31.ZḄViᢙ#LAஹOᢙBஹHᢙCஹOஹHᢙDஹOஹHᢙEஹᙳ┯ᫀD32.ᐵ$[UᎷᓫ^ḄAஹ2ᾱᩚZBஹCஹᨵDஹ⃩,⌕Eஹ᧕{Ὂ¢ឋᫀC33.ᑡᐵ$[UᎷᓫ^ZḄ£Aஹឋ¤Bஹ¥ឋ¤Cஹឋ◤Dஹ¥ឋ◤

37Eஹ☩ᐲ¨⁐□ឋᫀC34./Zmsᨬ23Ḅd¨%ḄAஹwBஹ¬ZCஹZDஹ!®¯ZEஹ/°ᫀD35.±²ZḄᢙd¨³´⌕µAஹᑖ¶·IgABஹIgGCஹIgMDஹIgEEஹ/¸
ᫀA36.ᑡQI~Zᐹᨵ¹᪀AஹXYZBஹᾯ᜼½ZCஹ[UᎷᓫ^ZDஹᑖ¾ᩚZEஹᜧᩚwZᫀB37.ᑡ~ZQIᐹᨵ¿À¹᪀Aஹᾯ᜼½Z

38BஹXYZCஹDஹ⊟Eஹcde⊈ᩚZᫀB38.ᙠᑡ~ZmsÂÃÄ%&Ḅd¨mᑖÅᑮḄAஹ▢ÈwᩚZBஹÉÊwᩚZCஹËYwᩚZDஹÌ#wᩚZEஹÍÎ⏚ᩚZᫀD39.ᡃij#᪗lms()oqrஹtuḄᜧwZxᦪzÒ|}5Aஹ1mlBஹ10mlCஹ100mlDஹ1000mlEஹ10000mlᫀC40.ZḄᢙ-᧕#ḄI&ᨵAஹH-OBஹS-RCஹV-WDஹ/¸
Eஹ/ᙳÔᫀD

3941.ᜧwᩚZᢣᦪᢣÖ()᪵ØmḄᜧwZxᦪAஹRᓣBஹÚᓣCஹᓣDஹÛÜÝEஹÛÛᑖÝᫀC42.⌕°ᜧwᩚwZ()ḄÞßd¨s◀á⌕°ᜧwᩚZ᜜sãKäåᦪæÔçz஺éᨵÖmlÞêmḄçZz2()L,ìᨵíîïðAஹ1000Bஹ10000Cஹ100000Dஹ1000000Eஹ10000000ᫀC43.ñZḄ᪶⏚sᨬ23ḄAஹófDôDNABஹófõឋDNACஹᓫfDôDNADஹᓫfõឋDNAEஹᓫfRNAᫀB44.öE*÷øᑴÜúû$ᐸýþwÿ
ᵨᐭᐭᙠᑴ

40!
"#$%&'◤⌕ᵨ*ᑡ,-.ᡂᑖ1
2ᜐᳮ5
Ḅ78ឋAஹ;<=⏘BஹEDTACஹ@ឋA⏚⏘DஹᩈD<=⏘EஹEF⏚⏘GᫀIA45.K2ឋLMNᵫ*ᑡ,-PQḄ
RSḄAஹT!
QBஹUVW
QCஹXY
QDஹZY
QEஹM
QGᫀIC46.[ᔾ⍝ᔠ
ᙠ_`abcdRS*ᑡ,-.efgeᙠḄhiAஹjᔠBஹkᔠCஹ᪶mnDஹo▲qEஹẚsGᫀIB47.tum
vwᡂᦔḄyᒹ{Aஹᜐᳮᑺ}

41Bஹ~ᱥᦪ}Cஹ"⏚@DஹᓄᢙᱥḄᙠEஹᙳGᫀE48.
◀78Ꮤᱥ᜜d78*ᑡ,.AஹBஹᱥCஹ⁚ᱥDஹ"ᏔᱥEஹ¡GᫀID49.¢£¤¥Ḅᨬ§⌕ḄdRS7ᑈḄ
◀K7
᜜ᒹ{*ᑡ,-.
Aஹ[ᔾ⍝ᔠ
BஹXK7
Cஹ©ª
Dஹ«
EஹLMN
GᫀID50.ᙠᡃ,¯°±W²³´⊈¶Ḅ%·¸¹⌕º*ᑡ,-.᧎¼Aஹ⇟¾Bஹ¿ÀCஹÁ¾DஹÂEஹÃ

42GᫀIE51.ÄÅÆwᙠ⌱ÈFSSÉᾦᐸwÌ´e´()⁐ᡈÑ⁐AஹÒÓBஹÔÓCஹ~ÕDஹÒÖEஹÔᮞGᫀIC52.*ᑡᔜÙÙÚÛᩚwQAஹÝÞ⏚ᩚwÙBஹ¯ßàwÙCஹ᰿âãwÙDஹäᱯwÙEஹÛᩚwÙGᫀID53.Ớ⏀ᐹᨵêÅëḄᩔíᵨᐸᵨîᑴAஹᢓᑴð⏘ñòᵨBஹẚóôCஹᓴᓄwö<=÷&øîdùuDஹᦋûwöüឋEஹýwö<=ῃÿᫀC54.ᵬḄᑺAஹᓄᑺBஹ

43CஹDஹᑺEஹ⏚ᫀD55.ᩭᑺAஹᓄᑺBஹCஹ⏣DஹᑺEஹᫀE56.⏚!ḄᑺAஹᓄᑺBஹCஹ⏣DஹᑺEஹᫀB57.ᵬḄ"ᵨᳮAஹᢓᑴ'(⏘*ឋBஹ,-./0Ḅ12⏚3ᔠ567ឋCஹᢓᑴ89:;Ḅ*ឋDஹ<=>?@5?ABCEஹ5>?ᑁ8Eᜫ*ᫀB

4458.⏣Ḅᩔ-"ᵨHᑴ◀JK᡾MNOPQ"ᵨ᜜⌕TAஹ5-.⏘ᜫ*Bஹ5-./0ῃV7ឋCஹᢓᑴ>?@ᔠᡂDஹXY-.Z=[Eஹᦋ7>?Ḅ]=ឋᫀB59.ᙠᑴᜓ`a2bOc◤ᵨe⁐ghOe⁐ᩭi᦮pHk,mᑴ᪗oe⁐gpcᵨqrstuAஹvewstuBஹx⏚stuCஹy⏚stuDஹTris⏚stuEஹz⏚z⏚stuᫀC60.cᵨELISA{|}~⏘3ᔠᱥ⊈ḄstustuAஹvewstuBஹx⏚stuCஹy⏚stuDஹTris⏚stuEஹz⏚z⏚stuᫀC61.ᙠ"ᵯb,᪵5ᵨḄstuAஹ⏚stuBஹTris-⏚stuCஹvewstu

45Dஹz⏚-z⏚stuEஹy⏚stuᫀC62.ᙠ"ELISA{|bOᡠᵨḄᨵᑭᔾ▬ᙠ⏘᪗ḄstuᒹuAஹ⏚stuBஹTris-HClstuCஹvewstuDஹz⏚-z⏚stuEஹy⏚stuᫀA63.|ᑁᵨ⏘ὶ{|ஹ}~⏘3ᔠᱥ⊈ḄstucᵨḄAஹvewstuBஹx⏚᠒᠒tuCஹy⏚stuDஹTRIS-⏚stuEஹz⏚-z⏚stuᫀC64.v¡hcᵨhO¢Ḅ⌕£A^ᵨ¤¥ᩔ¦§-Bஹᵨ¨¤¥©ᩔ¦§-ᩖ-«¬®ᱥḄE¯Cஹᵨ°±¥©²³ᩔ-Dஹ´µ¶ᣚᩔ-Eஹᵨ¤¥°9b¸ᩔ¦>-ᫀB

4665.¤[¹-bO¹-ᡂᦔ¬»,¤[¼½¥©[¾ஹb¸ᨵᐵO,¼½⛳Á©ᨵᐵO:Â⌕Ãᑮ¹-ᡂᦔ◤⌕A*᣸C┡ᑁᐰÈ´½Bஹ᣸C2/3´½ᓽÊCஹ᣸C1/2´½ËÌDஹ᣸C1/3´½ÍÌEஹ¬ᵨ᣸C´½ÍÌᫀA66.ÎK-ÏÐᐰKÑÒO◤ÓÔÕÖ×½ØᡂÙ×OÚᵨÙ×ÛÜÝÞᘤÝÞb,ÛÜqrà⁐áâãÙ×Aஹä⁐Bஹå⁐Cஹ0⁐Dஹæ⁐Eஹᐲè⁐ᫀD67.ÎKéᙠ-êuÖYᐭ᰿íᑺ᪵AஹᾙïBஹðïCஹῃᾦòóDஹ8ᳮVEஹy⏚stuᫀC68.-êuOᙠÎKé◤Y'{ᑺAஹKÑᑺ

47Bஹ᰿íᑺCஹ⚜ÎᑺDஹῃVᑺEஹÎ3ᑺᫀB69.>-õö.ᙠKÑᩩøù¬ú8ûüᦑAஹᜫJVᑖmᾦ7ឋBஹᜫJVᑖ/0E7ឋCஹᜫJVᑖVᓄᔠᱥ7ឋDஹᜫJVᑖ⏘7ឋEஹᜫJVᑖ89:;ᜫ*ᫀB70.ÿ
ᾦ⌱ឋ〉ᵨᱥAஹ⍝BஹCஹ᪶DஹḄᑖEஹ!"#ᫀ%D71.'()*+ᡠ◤Aஹ./0
ᾦBஹ123
ᾦCஹ456⌱ឋDஹᦋ89:;Eஹ<឵#ᫀ%D

4872.ẚ?@A,ᑖᡠ◤ᨬ〉Aஹ./0
ᾦBஹ123
ᾦCஹD⌱ឋDஹᦋ89:;Eஹ<឵#ᫀ%A73.⍝EḄᑖᡠ◤Aஹ./0
ᾦBஹ123
ᾦCஹ456⌱ឋDஹᦋ89:;Eஹ<឵#ᫀ%B74.FGᩚ◤⌕ᨬ〉AஹJᾙLMBஹNᜧ◭QCஹ1%R⊈
ᾦDஹTឋU⏗WEஹXᦋ8#ᫀ%C75.YZ␺\]Ẇ_EḄᱥ`ᱯឋb◤⌕cd,⌱〉eḄfgh⌕Ḅ᝞〉ᔠklmḄAஹJᾙLMBஹNᜧ◭Q

49cஹ1%R⊈
ᾦDஹTឋU⏗WEஹXᦋ8#ᫀ%B76.nᑡmpᨬ〉qrsᙠ25࠷ḄAஹu᝱mBஹwR⊈mCஹxmDஹy?mEஹz{m#ᫀ%E77.|klmᙠ᜜~pḄᢙ0nᑡpḄAஹᙠஹp,klmTBஹklmᙠឋᩩn⌕ᐸ⍝᧕TCஹpḄklmDஹklm|⏚¢£ᜧ¤┦Eஹklmᙠx¦§¨p©ª#ᫀ%D78.ᢙ⏚«⁐£E`®ᵨ¯°±,ᐸ²ᳮ´µᱥ¶ᐹᨵὊ⏚ឋºᱥ¶Aஹ»¼¶Bஹ½ᓄᔠᱥCஹᾦ¢Dஹ´µ¿ᱥ¶Eஹ´µ⏘¢#ᫀ%C

5079.☩ᐲ«⁐h⌕ḄE␺ë⁐°±ÄmÅÆ95%⏀ÈÉḄÊᵨAஹG-Ḽ⁐ᱞG+ῃ⁐BஹG-ῃ⁐G+Ḽ⁐ᱞCஹG-G+Îῃ⁐Dஹ|G-G+ÎÏῃ⁐ÊᵨEஹG-G=ÎÐÑÒÓ«ᡂᦔ#ᫀ%B80.klmÖ×pḄV-PØ×ÊYÙᑖnᑡµÚḄÛὃᢣ᪗°AஹßᐺáâELTorBஹO1â0139CஹO10139DஹãäÚâåãäæEஹçèÚâåçèÚ#ᫀ%A81.☩ᐲ«⁐Ḅéêëì%íîïÃEðñZòóôᐵö±᪵ᩭAஹᜧùᦪC+ḄᑁèQYü,G-᜜èQYüBஹᜧùᦪC-ýᑁèQYü,C+᜜èQYüCஹG+ᐹᨵ´⏘,G-þÿDஹG+
⏘,G-ᐹᨵEஹG+ᨵ,G-ᐹᨵᫀB82.᪶ᑖᩚᢙ⏚⁐□ឋḄᨬ"#$%Aஹ2ᩩ/mlBஹ5ᩩ/ml

51cஹ10ᩩ/mlDஹ15ᩩ/mlEஹ20ᩩ/mlᫀ'C83.(ᑡ*+,-〉ᔠ01+,Aஹ2ᜧ◭5+,-BஹBCYE6ᾦ+,-Cஹ89:;ᱥ+,@AB>a-C⊈EFGAஹHI᜼KBஹLMNᳫCஹ01DஹLMPQREஹLM⊟QRᫀB85.(ᑡ*=>ᱥᙠUVᓿXYZ>[\]AஹPQRBஹ01Cஹ^_⊟QRDஹLMNᳫEஹ`ab⊈ᩚᫀC

5286.cdefg^_⊟QR᪗i@jk᪵mn*ᢙ>5o⚜ᜐᳮAஹ☘◭5BஹN◭5Cஹt◭5Dஹᑭv;Eஹ2ᜧ◭5ᫀB87.␺x01@,mny⏘z{j6ᾦ|☢e~jᙠ[@ᑁᑨxAஹ20sBஹ30sCஹ60sDஹ90sEஹ120sᫀB88.fg01mnR-ᑁ⏒⏘z{,89.ᢥ᯿(ᑡ*-⁐ᓄᑨx%□ឋAஹt⁐%X⁐BஹX⁐%t⁐Cஹt⁐%⁐Dஹ⁐%t⁐Eஹt⁐%⁐ᫀB90.cd᪗i%ᓣ01Ḅ□ឋf᳛mn⏚ᜐᳮjk᪵ᵨ(ᑡ*ᱥAஹ20%HCL

53Bஹ30%HCLCஹpH2.2Ḅ0.2moI/LKCL.HCLDஹ0.2NH2SO4EஹINH2SO4ᫀC91.cdefg¡⊟QR᪗i@jk᪵mn*ᢙ>5o⚜ᜐᳮAஹ☘◭5BஹN◭5Cஹt◭5Dஹᑭv;Eஹ2ᜧ◭5ᫀB92.¢k£=¤¥ᢥ᯿ᐝ§¨⍝ªFG«¬ḄAஹkᐝ§£=¤Bஹᨩ¯°£=¤Cஹ₯ᐝ£=¤Dஹ²³´£=¤Eஹᵯ£=¤ᫀBA2⚪·93.¸¹º5»¼A-B·º5᪀,¾⍝¿IḄº5ᙳ»¼Á᪀஺¸¹º5⏘ÃឋÄᑖᙠÅᓫ³AஹA1BஹA2CஹB

54DஹAlÇBEஹAlÇA2ÈÉᫀA94.Ê˺5Ḅ¢>ᱥÃឋÌÍÎÏaÐÑÒÓ£F᪵ῳÕAஹÖºឋBஹÑ׺ឋCஹ¾ºឋDஹØe´eEஹØeÙ¥ÈÉᫀC95.Ê˺5ᵫAஹBÅᓫ³᪀ᡂ,AᵫAlஹA2᪀ᡂjᐸZᨵᢓᑴßàᔠᡂáᵨḄ¥AஹA1Åᓫ³BஹA2Åᓫ³CஹBÅᓫ³DஹØe´eEஹØeÙ¥ÈÉᫀAA3⚪·âkÑá␺x@jᢥ᯿ãä>ᓄåæzçឋèéz{ã␺x%ÊËê»96.ëkäá>ᓄåj◤íîÑá␺ïz{Aஹðឋ-ñòóôA-DóõBஹö:´Ḅ÷ø^êCஹA+B

55Dஹᜧ¾ùúêEஹØeÙâᫀC97.û>ᓄåüᔠËê»ýØÉxí4þækᓫþÿ⊈ᕈ□ឋὅᑣᐭAஹBஹCஹA+BDஹ₯ᐝEஹᑖ␺!ᫀ#E98.&ᐺ()*+,-./ᓄ1⊈2␺,4*ᵨAஹBஹCஹA+BDஹ₯ᐝ(ᳫEஹὊ8ឋ9:!ᫀ#BB1Aஹ;<=>?@BஹA<=>?@CஹQBDஹឫDE=>?@EஹF=>?@

56GᑡᔜJKEḄMNᑖOP99.ᙢRឋᦿTUV!ᫀ#B100.WXឋᦿTUV!ᫀ#AYZᢈ\]^_`ab91.ᙠdEefXgh,ijk⊈2l,Ag-AbᨵnoᱯRqrAg-Ab⌕tᱯRPAஹᑖhuឋBஹ*⌮ឋCஹw┦ឋyDஹᱯ:ឋEஹ&ᱯ:ឋ!ᫀ#D2.☩ᐲ<|⁐P~⌕Ḅ(␺O|⁐ᐸ|ᑺᓲᡀ<ỚᡠḄ.ᵨAஹ᤬|ᧇ.ᵨq᧕ῃ⁐Bஹ|(CஹdGῃ⁐f.ᵨDஹᩔ(Eஹẚ(!ᫀ#A3.⊈ᑴḄ¡¢i£■ῖᑺq■¦ᩖ(/¨,iᵨḄP©ª«iᵨ¬®Aஹ0.001%

57Bஹ0.01%Cஹ0.1%Dஹ1%Eஹ10%!ᫀ#B4.¯ᙠSS°ᾦ²/¨³Ḅᜧµ¶·<(³¸⁐Aஹ¹ºBஹ»ºCஹ¼½Dஹ¾Eஹ»ᮞ!ᫀ#B5.ᙠᵨ⊈2lgᡈfXWXE2ÁÂh,ijkAgÃAb,fXÄÅÆᨵᵯÈÉÊÃqᵯÈÉËÃᨵᐵ,ÍᵯÈÉγᨬЬhAஹÑᓣw┦ឋBஹÓÔw┦ឋCஹDஹ9EஹÑᓣᱯ:ឋ!ᫀ#A6.ÕÖ×ᳫ(ᙠØÙ²⌕tÚGᑡÛÜKEAஹᾱÞBஹᜧßឋÕÖCஹÕàáᓄDஹÕ⁚

58EஹãäZÖ!ᫀ#B7.⚩æ(ᢙMᡈè(ᙠéÜêjkAஹëìBஹ°ᾦ᡽ᦣCஹê1DஹEஹdWᵯï!ᫀ#D8.ᢙ⏚|⁐ḄñᑨAஹ(@Ḽᐲ⁐®ᢙ⏚(Bஹ(@Ḽº⁐®ᢙ⏚(Cஹ(@Ḽô⁐®ᢙ⏚(Dஹ(@Ḽ½⁐®ᢙ⏚Eஹ(@¾⁐®ᢙ⏚(!ᫀ#B9.GᑡÛÜõö÷〉ᔠúû(õöAஹüᜧ◭þõö÷BஹBCYE°ᾦõö÷Cஹÿ
DஹPPLOEஹKorthofᫀB10.ᐰ◤ᡂᵨ!"#$ᘤ#$&,!"()*+⁐-./

59Aஹ1⁐Bஹ2⁐Cஹ3⁐Dஹ4⁐Eஹᐲ6⁐ᫀD11.89:⌱<ឋ>?@6〉ᵨB)*CAஹDEFBஹ3ᗕᩚCஹẚJKᩚDஹLMNEஹOPᩚᫀE12.QឋO⏗STᾦCYE(〉ᵨB)*CAஹᑖWXYᩚBஹLMNCஹ3ᗕᩚDஹZ⍝CEஹ\]ḄᫀE13._ᾙab(cᵨḄdeᨵghᱯj()*Aஹk◤lmnḄCBஹkoᨵpḄCஹkoqpḄDஹ⌕lstḄCEஹkuvwxḄC

60ᫀc14.yᐲzḄ{ឋ|}~ᧇᙠᵯW᝱cᨵ□ᵯ₹CzᵯjᙠᜧpH᧕B{ឋ~ᧇᔠḼ⁐AஹpH12vBஹpH2-5CஹpH6-7DஹpH8-9EஹpH10-12ᫀB15.☩ᐲN~⁐◀ᡂᑖஹᩩᨵᐵ᜜,)*ᨵᐵAஹBஹᱏCஹ¡Dஹ¢oᢈ¤Eஹῃ⁐ᫀD16.95%Ḅ¦⏣¨©)*ª«¬70-75%Ḅ¦⏣¨©ᡂᦔ¯Aஹ95%¦⏣°±⊤☢´3µ¶·ឋ¸■º¦⏣»¼ᐭBஹµ¶ᢓᑴ⊤☢Ḅ⏘QឋᑁÂ⏘ÃᜫQCஹg¡¬ÅÆÇᢓᑴCÈÉᔠᡂÊ
¯DஹQÊ
¯Eஹ■ºCËÌÊ
¯ᫀA17.ÍYαÏᢈ¤¯ÐªᳮḄÒÓÔ⚗(ÐÖ×Ḅ

61AஹØÏ⏚(HauC14)ᙠªᑺoᵨkÜᔠᡂg¡ᜧÝḄÏ⚩ßàᡂáâᵯḄãÆΨ©BஹᵨªäÊåæçᙢéØÏ⏚ᑴᜓᔜÐìßíαÏ⚩ßCஹ¡ឋᡈï¡ðḄñ¶ÍY#$æä,αÏòÊåógôõö÷⚩ßḄøùúûᜧ,üý/ÍYÏ÷~⁐DஹαÏ⚩ßþÿ᧕ᔜᐳᔠEஹᜧḄᵯḄ⚩☟ᵯᵨᡂ!"#ḄḄ$%&᝱ᦑ)$%*ᫀ,D18../012ᒹ456ᘤ8./9:;./ᑖ=>ᑡ@ᱥBCD./ᑖ=AஹTCRᑖ=BஹMHCᑖ=CஹIgᑖ=Dஹ⊡%ᑖ=Eஹᵬ&GH*ᫀ,E19.IJKLMNO2SPAPᚈ@RSᔠAஹFCBஹFabCஹF(ab)2DஹTUVEஹWXV*ᫀ,A20.YZ9:[T9:ᵨᢙCDḄMcAb␺@CDᢙ^□ឋAஹCD8

62BஹCD4CஹCD3DஹCD2EஹCD1*ᫀ,A21.abAg-AbNOcdefghijNOklᨬnkḄAg,AbopqAஹ3,1Bஹ1,3Cஹ1,1Dஹ2,3Eஹ3,2*ᫀ,E22.AbrsklAஹtulvBஹwulvCஹNOxyDஹBNOEஹNOz{*ᫀ,A23.AgrzᜧsAஹᱯ}ឋ~ᓣBஹᱯ}ឋCஹ┦ឋ~ᓣDஹ┦ឋEஹ┦ឋ;ᱯ}ឋNOBX

63*ᫀ,D24.᪗Ḅ⚜ᜐᳮ᪵ab>ᑡᱥAஹlONNaOHBஹ20%HClCஹ14g/L⏚Dஹ0.9%NaClEஹ40g/LNaOH*ᫀ,E25.᫥[%ᨬ〉ᔠḄ᪗Aஹ⊈Bஹ>Cஹ᫥[DஹEஹ*ᫀ,B26.>ᑡ¡¢£¤ḄAஹ¥%¦B§¨ᩩª«f¬Bஹ¥%2ᨵ¯°¥%«fCஹ¯°¥%±²Ḅ³f´)µ¶%fDஹ¯°¥%d·¸¹ºEஹ¯°¥%»IFA¼¢*ᫀ,E27.᪶¬HLMs,72hw¾ᑨ!▢ឋᐸÂRÃÄÅᶖ!AஹW3mmBஹW4mm

64CஹW6mmDஹW8mmEஹ10mm*ᫀ,B28.ÈÉ=ḄÊËᨵAஹÌge⁐%Î᣸BஹÌgÐÑÎÒCஹÌgÐÑ᡽ÔDஹ⌼ᡂÖᜫØXEஹÙ¨ᙳ*ᫀ,E29.ÛÜݬԬÞßÐ᪵ᵨAஹᦻá°Bஹâឋ▰äCஹℍ⃔çè⏚é▰äDஹGNÔ¬EஹCEMÔ¬Þß*ᫀ,B30.ᐵD0139ëÛÜݬP01ëḄoì>íB£¤ḄAஹ0139ëḄ᪵îᱥïᱯðPO1ëElTorñoìòóḄBஹôὅ0ᢙ^Bc0ᢙ^Ḅ./⊈öôὅ÷øùJKCஹ0139ëÛÜݬ÷úûDஹ0139ëᨵüý§çEஹ0139ë¬þÿᨵ
01ḄᫀC

6531.☩ᐲ⁐⌕Ḅ␺⁐Ḅ!ᨔ#$%ឋᧇ,)*+ᵯ-ᙠ/0ᶖḄ᤬4%ឋᧇ56AஹpH1-2BஹpH2-3CஹpH3-4DஹpH4-5EஹpH5-6ᫀB32.!᪶ᩚḄᑖ;<=ᡠ◤<=Aஹ@ᾙBCBஹDᜧ◭<=Cஹ1%G⊈Iᾦ<=DஹKឋL⏗N<=EஹOᦋQ<=ᫀE33.RS◤⌕Ḅᨬ〉<=Aஹ@ᾙBCBஹDᜧ◭<=Cஹ1%G⊈Iᾦ<=DஹKឋL⏗N<=EஹOᦋQ<=ᫀD34.<=LVᩚᡠ◤<=AஹWXYIᾦ<=BஹZ[\Iᾦ<=

66Cஹ]^_⌱aឋ<=DஹᦋQbcd<=Eஹe឵<=ᫀC35.<=ᑭ/hijᡠ◤Ḅ<=AஹWXYIᾦ<=BஹZ[\Iᾦ<=Cஹ]^_⌱aឋ<=DஹᦋQbcd<=Eஹe឵<=ᫀE36.e឵<=ᡈlmᾙn<=〉ᔠp<=q$rsᱥAஹu<=Bஹᑭ/hijḄᑖ;<=Cஹv⍝Ḅ<=Dஹxy<=EஹRSḄ<=ᫀB37.uz{|ᢈ~$,ᐸ⌕iᳮ/0ᢝAஹ᠒{᠒|Bஹ{|Cஹ᠒{|Dஹ⌕{|ᓽEஹ{|ᫀB

6738.{ᢝrsᱥKYḄᦑAஹᦋᐸឋ¡Bஹᑁ£ᑖ¤ᡂ!ᨔCஹᑁ£ᑖ¤ᡂ!ᨔ¤ᡂ¦ᴻ¡Dஹ¨©᰿«Eஹis¬®ᑮ|᡾ᫀC39.±²³5|²³ᦔY⌕ᜧᐸᦑAஹµᓣ·¸Bஹ±²ᨵ¹º²»¼Yᜧ½¾¿ÀCஹẚÂ⏘ÄDஹ½ÅÆÄᑖÇEஹÅᜫKᫀB40.ᦋ¬ÉṹËᑡÍÎṹÏᨬÐÑÒḄÓÔAஹÕ᪶ᵞ⏚ØÙÚBஹᓄÜÝᑺÚCஹPCRÚDஹ#᜜àÚEஹáâã*ᫀA41.äå³ᡂᦔæçèᵨsᱥÔஹᓄÜÔ+஺ᐸësᱥÔAஹìíqᙳïðñòëäå¾ó<=sôᓽBஹìᨵõöḄïðñòëäå¾ó<=ôᓽCஹì÷á<=Ḅïðñòëäå¾ó<=ôᓽ

68Dஹìøùïðñòëäå¾ó<=ôᓽEஹì◤ùïðñòëäå¾ó<=ôᓽᫀB42.úûᔣýþ⊈¿ÝçÿḄIgG
ᓄ⊈ᳫᡠᵨAஹ⏚BஹTris-HCLCஹDஹ⏚⏚Eஹ⏚ᫀD43."#$%&'ᑺ)ᵨ*ᳮAஹᢓᑴ./⏘12)ᵨBஹ3456ᓄᔠᱥ9:;?4Ḅ@AឋDஹCDEᣚ)ᵨ345GHᜫJEஹ⌼ᡂ45ᑁᱥNᑖPẚRᫀC44.STUᐲWX&'ᑺᩔ4*ᑺAஹẚR45?Z[BஹẚR?Z\]ᢓᑴῃ_⏘JឋCஹ3GH`aDஹᢓᑴbcdDEஹ■f?49:ᫀB

6945.gᱥhiᑖCjklm5nᵨḄgᱥAஹoBஹpCஹqrDஹsHrEஹᜧHrᫀC46.uᑡᐵx᫥'lm5z{|}~ḄAஹ5᜜Bஹ5᜜ᩩuCஹᐢDஹᢙEஹ☘◭┦ᫀC47.&'ᵨᩔ%5|¡¢4ᡈᱯ¥¦bᱥ§¨ᢝ&'ᱥ|ᡠ◤Ὂ¬ᱥN>N᝞¯᝘./⏀²³&'nᵨḄ´Aஹ70.7࠷,40-60¶Bஹ71.7࠷,15-30¶Cஹ72.7࠷,5-10¶Dஹ73.7࠷,2-5¶Eஹ74.7࠷,1-2¶ᫀB48.·¸¹º»¼6᪗¾᝞¿ÀÁ〈ÃÄ■Å᪗¾`a,Çᙠ᪗¾|ÉᐭᱥNAஹINNaOH

70Bஹ20%HClCஹ4g/LËÌ⏚ÍDஹ0.9%NaClEஹ%4ÎÏ6ᫀ:C49.᪗ÑÒDNAḄ3'ÓÔknᵨT4Õ45DNAÖᔠ⏘×ᜧØᩚ4DNAÖᔠ⏘IKlenowᱏÛ⌕ÝᵫxßḄAஹ3,-5,᜜ᑗ᪶⏚⏘JឋâãBஹ5,-3,᜜ᑗ᪶⏚⏘JឋâãCஹÖᔠ⏘JឋâãDஹäåJឋæãEஹçJឋæãᫀA50.èé⊈ê`»hi⊈ê`»ëÇḄßìᵨxéḄÃíîḄ,èé`»ëÇÃí⊈ê|AஹAgBஹAbCஹïAbDஹðᐰAbEஹòóAbᫀA51.ÄôÃíAg,nᵨèé`»hiᙠèé`»hi|SPAÀõöIg÷ᔠAஹIgEBஹIgDCஹIgA

71DஹIgGEஹIgMᫀD52.᝞¿søᑴᜓḄDNAì▲ᑴ⏘ᑗûᑣýþu☢⚗ᜐᳮᨵ⚪AஹRNA⏘ᓄBஹ⏘KᜐᳮCஹ-Dஹ⏘ᜐᳮEஹSDSᜐᳮᫀC53-58⚪ᙠAg-Ab!"#$%⚩'ឋᢙ*⊈,-./53.ᡠ12Ḅ!4Aஹ56!Bஹ78!Cஹ9ᾦ᡽ᦣ!Dஹ=>!Eஹ?@ᵯB!ᫀD54.!CDEFGDᑨIAஹ++++Bஹ+++Cஹ++Dஹ+EஹLᫀC

7255.MN!O#ᙠPQRS"T4Aஹ10hBஹ8hCஹ6hDஹ4hEஹ2hᫀE56.VQCFT./WXYZ["QCFTᡠ\Ab4AஹIgMBஹIgACஹIgEDஹIgDEஹIgGᫀE57.QCFTḄ!QD4Aஹ65࠷Bஹ56࠷Cஹ37࠷Dஹ24࠷Eஹ4࠷ᫀC58.!"T4Aஹ24hBஹ12hCஹ6h

73Dஹ3hEஹ30ᑖᫀE59-62M:Aஹ_ᾙa▰Bஹcdஹefஹghg⏚jஹkl⏚fCஹmnDஹefEஹoឋpSS9ᾦqrs4tuvḄ⌱xឋqrs#ᡂᑖz{#ᜧ}ᑖ~⃩rᱥᢓᑴᑺ␺ᵨᢣᑺ஺ᐸoᑡᔜFᱥ59.ᢣᑺᫀE60.ᢓᑴᑺᫀB61.⃩rᱥᫀA62.␺ᵨnᫀC3.⊈ឋᳫ"#ᕜ12⊈#2~Aஹa⊈BஹB⊈CஹY⊈Dஹ6⊈

74Eஹe⊈ᫀB4.}[ឋℍ⃔ᳫ£{F⊈¤#ᐸo?¥ᨵ}[¦ᵨḄ⌕¨4Aஹa⊈¤BஹB⊈¤CஹV⊈¤Dஹ6⊈¤Eஹ£⊈¤ᫀA63.ᜧªᩚRNA¬ᔠ⏘Ḅ⌕¨®¯ᵫ5±²s³ᡂ#ᐸo()¶·¸¹ºḄ¦ᵨAஹa²sBஹB²sCஹV²sDஹ8²sEஹ£²sᫀD64.ᑭ¼½Ḅ¦ᵨ¾¿4RNA¬ᔠ⏘Ḅ()Aஹa²sBஹB²sCஹB'²sDஹ8²sEஹY²sᫀB

7565.ᨵ¾ᡂÀÁÂ᷈Äὅ¶[Æ᠒,ÉÊ"▣ÌឋᜮÎஹ⌲ÐÑᒓஹÓÔᨵÌÕஹូ×ஹᕏᔢஹᾯÛÜÝÞ□ឋ஺ᾯàáâᱏ#EᓺDåæZ⁐èéêÌëᨵìÛḄ⏗î®ï#ð¶ñFòZᨬḄô4Aஹ⁐õöBஹᵭøùºúCஹ᪷◭Dஹý◚ᳫEஹ☘◭
ᫀD66.ᯠᙠ38Cᡈᜩῳஹῳ!ᜧ#ᓝ%&஺()ஹ*+,-ូ/ᕏᔢᐸᨬ456Aஹញឋ9:;

7668.9Ḅ⃩?ᵨNᵫ9⊤☢Ḅ᡽ᦣ?ᵨIᡂḄ¥᡽ᦣ?ᵨḄ¦V§ᐵḄ©
NAஹ9ḄªḄ«¬ឋBஹᑁ᜜⃩ᱥ®¯VḄ°±Cஹ9Ḅ²ᨽ´µDஹᑁ⃩ᱥ®¶ᓄḄ¸VEஹ⃩ᱥ®N¹ºឋḄᡈᾦºឋᫀC69.ᙠPCR»¼@,DNAḄ½¾┯À᳛ÂÃÄFḄÅᦑNAஹÇᱥ¯V]ÈBஹPCR᡽ÉÊËᦪÌÍCஹ⌨ÏVÌFDஹTaqDNAÐᔠ⏘ÓÔ3'-5'᜜ᑗ⏘ÖឋEஹ×ØDNAḄÙÚᫀD70.ᐵÛ9⊈ÝÞßḄàáNAஹ9âᐭ⊈ãᙠᐸ@äåBஹᓄᾱឋ9âᐭ⊈ãᙠᐸ@äåCஹ9ᙠçèäåéêA
ëᐭ⊈ìDஹ9âᐭí⊈ìîïᙠᐸ@äåEஹ9᜜AAëᐭ⊈ìᫀD71.,ðᔠᾯªò᜼ô9᪗õö÷¥〈ùḄúᑣNAஹö÷᪗õüý§9þ?Bஹö÷᪗õ⌕
〈Cஹ᪗〈⌕ᢝஹᢝDஹ⌕ᙠḄᩞ

77Eஹᙠᵨᢙ#ᱥ%&ᩞ'ᫀ)C72.*+ஹ⏚ឋ.+/012⏚ឋ3ᧇᐸ6789ᵯ₹,=Ḅ>?ᑖ8ᨵBᵯ₹CᐸDᔠAஹᾦGBஹ1HCஹIJ⏚DஹKLMNOᑖEஹKLMNPᱥM'ᫀ)C73.QᑡS#ᱥ0ᵨTUV2WᶭYZ[ᳫYZAஹ☘◭_Bஹab_Cஹ᪍d◭_DஹIJᡈGEஹf◭_'ᫀ)D74.QᑡN,ghᙠ48ijklᓄno⏣qHḄrḄsAஹtuvwBஹxwyvwCஹzwyvwDஹ{ᑁwyvwEஹ}2~0s'ᫀ)D75.ᙠ᪶Lᱥ᪶iḄ⌕ḄN()ᙠᓄNᨬ0

78AஹHlBஹH2ACஹH2BDஹH3EஹH4'ᫀ)A76.kQsAஹᩚBஹᳫCஹᙊDஹEஹ'ᫀ)A77.¡¢£¤¢£¥¦wḄᑖ6Q§0B¨ḄsAஹᐸ⌱ªJdnSS«ᾦBஹd¬⊈¯ᑖ6°KCஹd¬±²᪗³ᑖ6Dஹ´Nd0gµEஹ¶·᪗□ឋ᳛ᙠo°º?ᕜj¼½¾¿'ᫀ)D78.ᙠÀJ2LÁÂᔁoḄsAஹÄÅÆÇᩚBஹᾙÉÊCஹᾪÌᩚDஹÍᩚEஹ∑᪵ÆÇᩚ

79'ᫀ)A79.ᙠ³ÐÑ⊈ÒÓÔÕÖ×°jØᵨḄAg-AbÙÚÛÜ_ᨵ1ÝsᐸN%?ÞÙÝ〉Þà¿j,Ag—AbÙáÂAஹàâBஹ0ãCஹäåDஹæ᠒Eஹè6'ᫀ)A80.éHêJëTAஹJẠJBஹ␺îJCஹ⃩JDஹ⌱ªJEஹðñJ'ᫀ)B81.óôõö÷Õsᨵᦔ÷ÔÕ%?÷ḄᡂᦔúTõöôûஹÝüjý,?᪵ᨬi◤1ᜧ
ᢝ15-20ᑖ,ᩔᒹᙠᑁᱥAஹo.5kg/cm2Bஹ0.8kg/cm2C^1.0kg/cm2D^1.05kg/cm2Eஹ1.5kg/cm2ᫀD

8082.ᙠCFT⌕!⊈#$%⊡',()*+ᱥ⊈#Ḅ⊡'$%-./Aஹ65࠷Bஹ56࠷Cஹ37࠷Dஹ1*ḄEஹ)*ḄᫀE83.3᪶ᑖ5ᩚ78ᢙ⏚;⁐□ឋḄᨬ@A.BA^2ᩩ/ml5ᩩ/mlCஹ10ᩩ/mlDஹ15ᩩ/mlEஹ20ᩩ/mlᫀDC84.ᑡF7Gᨬ〉I-.ᙠ25࠷Ḅ/AஹJ᝱F7BஹLM⊈F7CஹNF7DஹOPF7EஹQRF7ᫀE85.STF7ᙠ᜜VGḄᢙW
ᑡXYG)Z[Ḅ/Aஹᙠ\]ஹ\^_`GSTa7b%cde

81BஹSTF7ᙠmឋᩩn⌕oᐸfg⍝7ij᧕b%CஹqrstGḄSTF7uvlDஹSTF7⏚wx)ᜧz┦EஹSTF7ᙠNt|}~GᫀD86.ᙠ7Gᐭ᰿ᑺ᪵/:AஹᾙBஹCஹῃᾦDஹᳮ]tEஹ⏚ᫀC87.ᵬḄᵨ¡ᳮAஹᢓᑴ¥¦⏘w%ឋBஹ¨7'©ªḄ«¬⏚3ᔠs®¢ឋCஹᢓᑴI¯°Ḅ%ឋDஹ±²³´sµ¶·Eஹs³ᑁ¡¸ᜫ%ᫀB88.º⏚»/¼½¾wḄᑺAஹ¿ᓄᑺBஹÁ¼]w

82cஹ⏣wDஹÄ¡ᑺEஹÅwᫀB89.ᙠᡃÇÈÉÊËÌÍ·⊈ÎḄÏÐÑÒ⌕ÓBᑡ¾᧎ÕAஹ⇟°Bஹ×ØCஹÙ°DஹÚEஹÛᫀE90.ÜÝÞḄᨬÁ⌕ḄßàáâãᑈḄå◀çãå᜜Äᒹᑡ¾åAஹèᔾ⍝ᔠåBஹLçãåCஹêëåDஹìåEஹíîïåᫀD91.ᙢñឋᦿëPóḄ⚜■öAஹ$÷ஹ$⇟BÁ⌕øùBஹ$úஹ$⇟BÁ⌕øùCஹ$ûஹ$ØBÁ⌕ឋøùDஹ$ÚBÁ⌕øùEஹ$∫ஹ$∭BÁ⌕øùᫀC

8392.ᡃÇþ᪗
()ஹḄᜧᦪ5Aஹ1mlBஹ10mlCஹ100mlDஹ1000mlEஹ10000mlᫀC93.ᙠᑡ
!Ḅ"#
ᑖ%ᑮḄ'Aஹ▢*ᩚBஹ78ᩚCஹ9:ᩚDஹ;/ᩚEஹ<=⏚ᩚᫀD94.ᑡ,-./ᱥᐹᨵ3ᾱ5Aஹ?:Bஹ@3ᎷᓫCCஹDEFGHᩚDஹI"J⊈ᩚEஹDELᳫᫀB95-98⚪AஹNᾦ᡽ᦣRS(4PTU)BஹCFTCஹ⊈VRWXYRS

84DஹZCFTEஹ[\]^ᑡᔜ-`aḄbcᑖd'95.24ebᫀC96.30ᑖᫀB97.3-7ᑖᫀE98.72ebᫀA99."#ᑡfghiᱞklmnḄ'AஹETECBஹEPECCஹopDஹqrstuEஹvwxyzᫀD100.ᑡᐵ|DEFGHᩚḄ}~yzḄ'Aஹ☩ᐲu#⁐□ឋBஹᐹᨵ᪀Cஹឋ◤DஹឋEஹ⃩◤yzᫀB