DB43∕T 2390-2022 小龙虾人工繁育技术规程(湖南省)

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ICS65.150CCSB5243湖南省地方标准DB43/T2390—2022小龙虾人工繁育技术规程Codeofpracticeforartificialbreedingofcrayfish2022-07-08发布2022-10-08实施湖南省市场监督管理局发布

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2DB43/T2390—2022目次前言························································································································Ⅲ1范围·····················································································································12规范性引用文件······································································································13术语与定义············································································································14环境条件···············································································································15主要设施···············································································································16亲虾培育···············································································································27人工催产···············································································································28控温孵化···············································································································39苗种培育···············································································································310捕捞与运输···········································································································311生产记录··············································································································4参考文献····················································································································5I

3DB43/T2390—2022II

4DB43/T2390—2022前言本文件按照GB/T1.1—2020《标准化工作导则第1部分：标准化文件的结构和起草规则》的规定起草。请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。本文件由湖南省农业农村厅提出。本文件由湖南省农业标准化技术委员会归口。本文件起草单位：湖南省水产科学研究所、湖南风向农业科技发展有限公司、湖南助农农业发展有限公司、顺祥食品有限公司、湖南中闳生态农业科技发展有限公司。本文件主要起草人：何志刚、王冬武、田兴、李金龙、谢仲桂、邓时铭、刘丽、何咏、王湘华、刘训华、任维、黄庆明、周顺祥、胡立军。III

5DB43/T2390—2022IV

6DB43/T2390—2022小龙虾人工繁育技术规程1范围本文件规定了克氏原螯虾（Procambarusclarkii）（以下简称小龙虾）人工繁育的环境条件、主要设施、亲虾培育、人工催产、控温孵化、苗种培育、捕捞与运输、生产记录的技术要求。本文件适用于小龙虾人工繁育。2规范性引用文件下列文件中的内容通过文中的规范性引用而构成本文件必不可少的条款。其中，注日期的引用文件，仅该日期对应的版本适用于本文件；不注日期的引用文件，其最新版本（包括所有的修改单）适用于本文件。GB3838地表水环境质量标准GB11607渔业水质标准GB13078饲料卫生标准NY/T391绿色食品产地环境质量NY/T471绿色食品饲料及饲料添加剂使用准则SC/T1135.4稻渔综合种养技术规范第4部分：稻虾（克氏原螯虾）SC/T6093工厂化循环水养殖车间设计规范DB43/T634畜禽水产养殖档案记录规范DB43/1752水产养殖尾水污染物排放标准3术语和定义本文件没有需要界定的术语和定义。4环境条件场地环境应符合NY/T391的规定。水源水质应符合GB3838（Ⅲ）与GB11607的规定。5主要设施5.1亲虾培育设施宜选用规格为2m×2m×1.5m或2m×3m×1.5m、网目网眼规格为3mm的聚乙烯无结网箱，用浮筒架设，每排网箱留0.5m～1.0m人工通道，每两排网箱留2m宽水道，培植水雍菜、水芹菜等22水生植物。网箱设置在面积3000m～6000m的池塘中，池塘水深2m左右，进排水独立设置。网箱面积占池塘总面积的1/4～1/3。1

7DB43/T2390—20225.2繁殖设施5.2.1大棚22宜采用钢架塑料大棚，应能调光、保温、防雨和通风，面积1000m～1500m。5.2.2配对池在大棚内建2m×4m或3m×6m的水泥池，池深1m左右，池底坡比3％～5％。占大棚面积30％左右。5.2.3孵化池在大棚内建5m×10m或5m×15m的水泥池，内壁光滑，进排水口用网眼规格0.6mm的过滤网双层套袋过滤，池底四周高，中间低，坡比3％～5％，在池底最低处设置出水口、集虾槽及水位保持装置。孵化池占大棚面积60％左右。5.3苗种培育设施22在大棚外按孵化池面积1∶10配套建设苗种标粗池，单池面积为500m～1000m，池深1m左右。池底一端设内口径为10cm～20cm的排水管。5.4配套设施配备控温、控光、供电、充气和水处理设施，均应符合SC/T6093的相关技术要求。6亲虾培育6.1后备亲虾选择应从有资质的苗种场采购，经检验检疫合格。种虾应附肢齐全、无损伤、体表无附着物，体格健壮、活动能力强；体长6cm以上，雌虾体重不低于30g/只，雄虾体重不低于35g/只。6.2培育22雌、雄亲虾应分箱培育，培育密度为15尾/m～20尾/m。投喂亲虾专用饲料（粗蛋白质不低于30％，赖氨酸不低于1.40％，粗脂肪不低于5％），饲料符合GB13078和NY/T471的规定。雌虾定期拌喂鱼糜或维生素E，促进性腺发育。水质应保持pH8.0～8.3，氨氮小于0.4mg/L，溶解氧大于5mg/L。7人工催产7.1亲虾挑选8月底～9月初，检测雌虾卵巢发育情况，待卵巢发育到Ⅳ期个体达80％以上时，进行人工催产。7.2药物催产可使用人绒毛膜促性腺激素（HCG）催产，每只雌虾100IU～150IU，雄虾剂量减半。注射部位为腹部第二体节，注射时针头与腹部呈30度角左右，深度为3mm左右。2

8DB43/T2390—20227.3配对22将注射后的亲虾，按雌雄比（2～3）∶1配对放入产卵池，放养密度20只/m～30只/m，产卵池保持微流水，溶解氧5mg/L以上。7.4抱卵配对48h后，应将雄虾移走，雌虾抱卵后转移至孵化池，集中孵化。8控温孵化8.1抱卵虾投放22放养密度40只/m～50只/m，水温控制在20℃～25℃，溶解氧5mg/L以上。8.2日常管理受精卵开始脱膜，可适量投喂亲虾专用饲料。待稚虾大多数离开母体可自由游动时，将亲虾移走，进行稚虾培育。9苗种培育9.1稚虾培育饵料以培育浮游生物为主，适当投喂小龙虾开口粉料。15d～20d后，稚虾体长2cm～3cm时，可出售或分池培育。9.2幼虾培育22宜在室外苗种培育池进行，放养密度为300尾/m～500尾/m，以投喂小龙虾幼虾配合饲料为主，饲料符合GB13078和NY/T471的规定。培育至次年3月上中旬，幼虾体重2g～3g时，可起捕出售或转塘养殖成虾。9.3日常管理培育期间定期换水、增氧，透明度保持30cm～40cm，溶解氧5mg/L以上。尾水排放按DB43/1752的规定执行。9.4病害防控按照SC/T1135.4的规定执行。10捕捞与运输10.1捕捞稚虾宜采用排水法，用网具在集虾槽或排水口收集；幼虾宜采用网眼规格为1.6cm的地笼捕捞。10.2运输稚虾带水充氧运输，幼虾塑料框铺水草保湿运输。3

9DB43/T2390—202211生产记录按DB43/T634的规定执行。4

10DB43/T2390—2022参考文献[1]GB/T20014.15—2013良好农业规范第15部分：水产工厂化养殖基础控制点与符合性规范[2]GB/T30890—2014凡纳滨对虾育苗技术规范[3]SC/T1144—2020克氏原螯虾[4]SC/T2096—2019三疣梭子蟹人工繁育技术规范[5]T/HNSX001—2020克氏原螯虾（小龙虾）配合饲料5