lekkerkerker human antibodies to dendritic cells generation, analysis and use in vaccination外语英文电子书

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1、HUMANANTIBODIESTODENDRITICCELLSGENERATION,ANALYSISANDUSEINVACCINATIONHUMANANTIBODIESTODENDRITICCELLSGENERATION,ANALYSISANDUSEINVACCINATIONHUMANEANTILICHAMENTEGENDENDRITISCHECELLENOntwikkeling,analyseengebruikinvaccinatie(meteensamenvattinginhetNederlands)PROEFSCHRIFTterverkrijgingvandegraadvando

2、ctoraandeUniversiteitUtrechtopgezagvandeRectorMagnificus,Prof.dr.W.H.Gispen,ingevolgehetbesluitvanhetCollegevoorPromotiesinhetopenbaarteverdedigenopvrijdag31mei2002desmiddagste2.30uurdoorAnnemarieNicoletteLekkerkerkergeborenop6december1972,teAsse,BelgiëPromotorenProf.dr.T.LogtenbergProf.dr.H.Cle

3、versCover:‘BlackHollyhockwithBlueLarkspur’GeorgiaO’Keeffe1929ISBN:90-393-3038-7ThestudiesdescribedinthisthesiswereperformedattheDepartmentofImmunology,UniversityMedicalCenterUtrecht,TheNetherlandsandwerefinanciallysupportedbyCrucellN.V..Whenyoutakeaflowerinyourhandandreallylookatit,it’syourworld

4、forthemoment.GeorgiaO’KeeffeCONTENTSCHAPTER1Generalintroduction9CHAPTER2APhageantibodiesagainsthumandendriticcellsubpopulationsby33flow-cytometrybasedselectiononfreshlyisolatedcellsCHAPTER2BPhageantibodiesselectedondendriticcellsinhumantonsil51CHAPTER3ReactivityprofilesofhumanIgG4monoclonalantib

5、odiesonnaturally69occurringandcultureddendriticcellsubsetsCHAPTER4BindingandcrosslinkingofhuMabMatDC64preventstheinvitro97differentiationofhumanmonocytesintodendriticcellsCHAPTER5Invitroantibody-mediateddeliveryofmelanomatumorantigenMAGE-1111toimmaturedendriticcellsresultsinanti-tumorTHandCTLres

6、ponsesCHAPTER6Lipid-taggedscFvfragmentsincorporatedintumorcellsmediate131receptor-dependenttargetingtoantigenpresentingcellsCHAPTER7Summarizingdiscussion145NederlandseSamenvatting155CurriculumVitae157ListofPublications158Nawoord159CHAPTER1GENERALINTRODUCTIONGENERALINTRODUCTIONINTRODUCTIONThelife

7、cycleofdendriticcells1Dendriticcells(DCs),firstdescribedin1973bySteinmanandCohn,arenowwidelyrecognizedasprofessionalantigenpresentingcells(APCs)thatplayapivotalroleindirectingtheimmuneresponse.TheabilityofDCstoprocessandpres