genetic homogeneity of in vitro raised plants of grapevine cv. crimson

《genetic homogeneity of in vitro raised plants of grapevine cv. crimson》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。

1、Availableonlineatwww.sciencedirect.comSouthAfricanJournalofBotany78(2012)302–306www.elsevier.com/locate/sajbShortcommunicationGenetichomogeneityofinvitroraisedplantsofgrapevinecv.CrimsonSeedlessrevealedbyISSRandmicrosatellitemarkersA.Nookaraju1,D.C.Agrawal⁎PlantTissueCultureDivision,CSIRNati

2、onalChemicalLaboratory,Dr.HomiBhabhaRoad,Pune–411008,IndiaReceived10May2011;receivedinrevisedform17August2011;accepted18August2011AbstractThepresentstudywasconductedtotesttheclonalhomogeneityofsixmontholdtissuecultureraisedplantsofgrapevinecv.CrimsonSeedlessusingInterSimpleSequenceRepeat(ISS

3、R)andSimpleSequenceRepeat(SSR)markers.Visibleassessmentoftheseinvitroraisedplantsmain-tainedinpolyhousedidnotshowanymorphologicaldifferencesamongthemselves.However,totestthegenetichomogeneityoftheseplants,wescreened50ISSRprimersoutofwhich,22primersproducedscorableandrepeatablebands.These22pr

4、imerswereusedfurtherforassessingge-netichomogeneityofinvitroraisedplantsofCrimsonSeedless.These22ISSRprimersgenerated134distinctbandclasseswithatotalof3216scorablebands.Alltheprimersshoweduniformbandingpatternforalltheinvitroraisedplantsandthemotherplant.Incaseof5SSRprimers(VS1,VVMD5,VVS2,VM

5、CNG4c8andVVMD31)used,atotalof288scorablebandswereobtained.Theallelesizesrangedfrom98to254bp.Alleliccompositionof23invitroraisedplantsandthemotherplantat5SSRlocididnotshowanypolymorphism.Theresultsofthetwomarkersystemsinthepresentstudyrevealedthegeneticuniformityamongtheinvitroraisedplantsdem

6、onstratingthereliabilityofinvitropropagationsystemusedforthecultivar.©2011SAAB.PublishedbyElsevierB.V.Allrightsreserved.Keywords:Allelecomposition;Clonalfidelity;InterSimpleSequenceRepeats;Monomorphicbands;Tissueculture;VitisviniferaL.1.Introductionduration,temperature,pHetc.),theoccurrencean

7、dextentofinvitrogeneticinstabilitymayalsobehighlygenotypespecificTissuecultureenvironmenthasbeenreportedtocausegeneral(Vázquez,2001).Ingeneral,micropropagatedplantsobtainedalterationsincellularcontrols,leadingtogenomicchangesinthefrompre-formedmeri