advanced analysis algorithms for microscopy images

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1、AdvancedAnalysisAlgorithmsforMicroscopyImagesSihengHeSubmittedinpartialfulllmentoftherequirementsforthedegreeofDoctorofPhilosophyintheGraduateSchoolofArtsandSciencesColumbiaUniversity2016ProQuestNumber:3742913AllrightsreservedINFORMATIONTOALLUSERSThequalityofthisreproductionisdependentuponthe

2、qualityofthecopysubmitted.Intheunlikelyeventthattheauthordidnotsendacompletemanuscriptandtherearemissingpages,thesewillbenoted.Also,ifmaterialhadtoberemoved,anotewillindicatethedeletion.ProQuest3742913PublishedbyProQuestLLC(2015).CopyrightoftheDissertationisheldbytheAuthor.Allrightsreserved.Th

3、isworkisprotectedagainstunauthorizedcopyingunderTitle17,UnitedStatesCodeMicroformEdition©ProQuestLLC.ProQuestLLC.789EastEisenhowerParkwayP.O.Box1346AnnArbor,MI48106-1346©2015SihengHeAllrightsreservedAbstractAdvancedAnalysisAlgorithmsforMicroscopyImagesSihengHeMicroscopeimagingisafundamentalexp

4、erimentaltechniqueinanumberofdiverseresearchelds,especiallybiomedicalresearch.Itbeginswithbasicarithmeticoperationsthatintendtoreproducetheinformationcontainedintheexperimentalsample.WiththerapidadvancementinCCDcamerasandmicroscopes(e.g.STORM,GSD),imageprocessingalgorithmsthatextractinformati

5、onmoreaccurateandfasterarehighlydesirable.Theoverarchinggoalofthisdissertationistofurtherimproveimageanalysisalgorithms.Asmostofmicroscopeimagingapplicationsstartwith uorescencequantication,rstwedevelopaquanticationmethodfor uorescenceofadsorbedproteinsonmicrotubules.Basedonthequantiedresu

6、lt,theadsorptionofstreptavidinandneutravidintobiotinylatedmi-crotubulesisfoundtoexhibitnegativecooperativityduetoelectrostaticinteractionsandsterichindrance.ThisbehaviorismodeledbyanewlydevelopedkineticanalogueoftheFowler-Guggenheimadsorptionmodel.Thecomplexadsorptionkineticsofstreptavidintobi

7、otinylatedstructuressuggeststhatthenanoscalearchitectureofbindingsitescanresultincomplexbindingkineticsandhenceneedstobeconsideredwhentheseintermolecularbondsareemployedinself-assemblyandnanobiotechnology.Inthesecondpart,apowerfullock-i