最新农业植物病理学专题报告 (2)课件ppt.ppt

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1、农业植物病理学专题报告(2)ThisProgramfundedby：HenanOutstandingYouthFund，andTheFundforCASTrialStationofAgriculturalEcologyPart1.BackgroundCollectionofChemicalElicitorsSecuritytest2.Outline：TestinducedeffectinpotTestinducedeffectinfliedmechanismInhibitioneffectonpathogenChangesofenmyces，Protein，pat

2、hotoxinPart2.Materialsandmethods2.1Materials★ChemicalElicitors：Chitosan,SA（2-(acetyloxyl)-Salicylicacid）ethephonCCC（chlorocholinechloride）Oligoglucose,BTH(Benzothiadiazole-7-carbothioic),Procainehydrochloride.OxalicacidDPC(N,N-dimethylpeperidiniumchloride)★Cottoncultivar：Zhongmian17，s

3、usceptible★Pathogenstrain：VerticilliumdahliaeHN98-12.2UsingofChemicalElicitorsChitosan100μg/ml2timesSA100μg/ml2timesOligoglucose1000μg/ml2timesBTH100μg/ml2timesProcainehydrochloride80μg/ml2timesOxalicacid100μg/ml2timesDPC50μg/ml1timesethephon50μg/ml1timesCCC100μg/ml1times2times：seedli

4、ngstage+squarestage；1times：seedlingstageTheinhibitionofchemicalelicitorstothegrowthofmyceliumandsporesgerminationofVerticilliumdahilaeweretestedinvitro.growthofmycelium：——myceliumdishsporesgermination：——suspenddrop2.3Theinhibitionofchemicalelicitorstothegrowthofmyceliumandsporesgermin

5、ation2.4Theeffectofchemicalelicitorstobiochemistryofcottonplants—Peroxidase(POD)，Phenylalanineaminolyase(PAL)activityandtheContentsofhemi-gossypolweremeasuredbyspectrophotometer；—Water-solubilityproteinswasanalyzedbySDS-PAGEPart3.Results133.1Theeffectofchemicalelicitorsoncontrolcot

6、tonVerticilliumwilt3.1.1inpot3.1.2infieldTable1.TheeffectofchemicalelicitorsoncontrolcottonVerticilliumwilt（inpot，bellstage）TreatmentsIncidence%Indexofdiseaseeffect%Chitosan2.71.4*79.7SA8.93.352.5Oligoglucose7.83.648.1BTH15.84.830.6Procainehydrochloride8.33.154.8Oxalicacid6.73.351.7DP

7、C3.31.7*75.8ethephon3.32.5*63.8CCC11.74.735.2Non-treatment（CK）11.76.9——Table2.TheeffectofchemicalelicitorsoncontrolcottonVerticilliumwilt（inpot，harveststage）TreatmentsIncidence%Indexofdiseaseeffect%Chitosan23.412.8**63.1SA43.527.520.7Oligoglucose37.014.3*58.7BTH29.216.0*53.8Procainehy

8、droch