生化仪比色杯报警原理.ppt

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1、Photo-CalWhatisa“Photo-cal”Photo-cal(Photocalibration)Photo-calisCuvetteBlanking(CellBlank)CheckCuvetteconditionAdjust“ZeroBase”forPhotometerIttakesabout20minWhatisa“Photo-cal”DispenseDIwaterfromReagentProbeToallCuvettes1)CheckCuvettecondition2)AdjustZERObaseHowtod

2、o?CuvetteCheckAfterfinish“Photo-cal”,pleasechecktheCuvetteconditionAnydirtyCuvette??AnyScratchoncuvette??CuvettecheckSelect“CuvetteStatus”CuvettecheckRecommendRangeMean0.03Abs.0.10PressthisCuvettecheckThereareThreetypeofcheckingmethods.MeancheckAbsorbancecheckCuv

3、ettecheckIfthereisanyproblematiccuvettes,AUshowserrorMeancheckRedcolorAbsorbancecheckBluecolorCuvettecheckGreencolorCheckErrorCuvetteErrorCuvette(DirtyCuvette)WhatisMeanCheck?OpticalDensity(OD)ofeachCuvetteMean-0.03+0.03Morethan+/-0.03OutofRangeDirtyCuvetteWhati

4、sAbsorbanceCheck?PreviousPhotocalDataDirtyCuvetteNEWPhotocalDataMorethan+/-0.100OutofRangeOpticalDensity(OD)ofpreviousPhotocal0.100CuvetteCheckIfthereareanyErrorCuvette,youhavetocleanorreplacethecuvetteHowtochangetheCuvetteHowtochangetheCuvetteCuvetteNumberCuvetteC

5、hangeYouhavetodo“Photo-cal”again,whenyouclean/replacethecuvettePhoto-calClean/replacecuvettePhoto-cal(2ndtime)