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时间:2021-01-16
《静电纺丝纳米纤维膜固定化酶及其应用.docx》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、第22卷第9期化学进展Vol.22No.92010年9月PROGRESSINCHEMISTRYSep.,2010静电纺丝纳米纤维膜固定化酶及其应用*代云容牛军峰殷立峰刘佳蒋国翔(北京师范大学环境学院水环境模拟国家重点实验室北京100875)摘要静电纺丝是一种简单有效的制备聚合物纳米纤维的技术,在组织工程、药物控释和传感器等方面具有广泛的应用。采用静电纺丝技术制备得到的纳米纤维膜具有比表面积大、孔隙率高和易于分离回收等优点,可以作为一种优良的酶固定化载体,目前在酶固定化领域受到了广泛的关注。本文综述了近年来静电纺丝纳米纤维膜固定化酶的研究
2、进展,在阐述静电纺丝纳米纤维膜制备技术的基础上,详细介绍了纳米纤维膜表面担载法和包埋法固定化酶的原理和方法,分析了不同固定化方法的优缺点,并讨论了静电纺丝纳米纤维膜固定化酶的应用前景,对静电纺丝纳米纤维膜固定化酶的发展方向进行了展望。关键词静电纺丝纳米纤维膜酶表面担载法包埋法中图分类号:O631.3;TQ340.64;Q55文献标识码:A文章编号:1005-281X(2010)09-1808-11ElectrospunNanofiberMembranesasSupportsforEnzymeImmobilizationandItsApplicati
3、onDaiYunrongNiuJunfengYinLifengLiuJiaJiangGuoxiang(TheStateKeyLaboratoryofWaterEnvironmentSimulation,SchoolofEnvironment,BeijingNormalUniversity,Beijing100875,China)AbstractElectrospinningisconsideredasasimpleandeffectivetechnologyforproducingpolymernanofibers,whichhasbeenwi
4、delyusedintissueengineering,drugreleaseandsensors,etc.Thenanofibermembranesobtainedfromelectrospinningexhibitmanyextraordinarypropertiesincludinghighsurface-to-volumeratio,porousstructureandreusability.Duetotheseexcellentfeatures,electrospunnanofibermembranesbecometheoutstandi
5、ngcandidatesforenzymeimmobilization,andhaveattractedextensiveattention.Onthebaseofbrieflyclarifyingthepreparationtechniqueofelectrospunnanofibermembranes,thisreviewmainlysummarizestherecentadvancesinusingelectrospunnanofibermembranesassupportsforenzymeimmobilizationbytwodiffer
6、entmethods,i.e.surfaceimmobilizationandencapsulation.Surfaceimmobilizationreferstophysicaladsorptionorchemicalattachmentofenzymesonpristineormodifiedelectrospunnanofibermembranes,andencapsulationmeansimmobilizingenzymesinelectrospunnanofibermembranesthroughelectrospinningthemi
7、xtureofenzymeandpolymer.Theadvantagesanddisadvantagesofdifferentimmobilizationmethodsareanalyzed.Theapplicationsoftheenzymeimmobilizedelectrospunnanofibermembraneinbioreactors,biosensorsandthetreatmentofenvironmentalpollutantsarediscussed,andtheprospectsofthisresearchfieldarea
8、lsopresented.Keywordselectrospinning;nanofibermembranes;enzym
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