-TOYOPEARL填料在血浆蛋白中的应用.ppt

《-TOYOPEARL填料在血浆蛋白中的应用.ppt》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。

1、血液制品层析工艺中TOYOPEARL填料东曹(TOSOH)公司生命科学部门.PlasmaProductsReviewApplicationonPlasmaProteinContent2.ProteinConcentrationinPlasmaIndicationAlbumin40g/LVolumerestorationaftertrauma,shock,burnsAlpha1ProteinaseInhibitor1.5mg/mLHereditaryemphysemaAnti-DIgGTitervariesaRhprophylaxisinpregnancyandchildbirthAntithr

2、ombinIII100ug/mLAntithrombinIIIdeficiencyC1-inhibitor170ug/mLHereditaryangioedemaFactorIX10ug/mLHemophiliaBFactorVIII0.5ug/mLFactorVIIdeficiencyFactorXI0.3ug/mLHemophiliaBFactorXIII30ug/mLFactorXIIIdeficiencyFibrinogen3g/LTissuesealantcomponentFibronectin300ug/mLWoundhealingHepatitisBIgGTitervariesa

3、HepatitisimmunityImmunoglobulinGUpto12.5g/LPrimaryandsecondaryimmunedeficiencyMeaslesIgGTitervariesaMeaslesprotectionandtreatmentProteinC4ug/mLNeonatalthrombosisRabiesIgGTitervariesaRabiesriskTetanusIgGTitervariesaTetanusprotectionandtreatmentThrombin150ug/mLbTissuesealantcomponentVaricellaZosterIgG

4、TitervariesaChickenpoxprotectionVonWillebrandFactor10ugVonWillebrand’sdiseaseaTiters(antibodyconcentrations)varyinug/mLranges.bAsprothrombinPlasmaProteinsofCurrentInterest3.PlasmaProductsinChina4.PlasmaprecipitationsupernatantCryoprecipitateFactorVIIIFractionICentrifugation4oCEthanoltreatment,Centri

5、fugationsupernatantprecipitationFibrinogenFactorIXAbtithrombinIIICohnColdEthanolFractionationProcess(Continued)5.precipitationsupernatantFractionIVFractionVEthanoltreatment,CentrifugationsupernatantprecipitationHaptoglobinAbtithrombinIIIAlbuminFractionII+IIIsupernatantprecipitationprecipitationEthan

6、oltreatment,CentrifugationFractionIIGlobulin(forintramuscular)Globulin(forintravenous)EthanoltreatmentEthanoltreatment,CentrifugationCohnColdEthanolFractionationProcess6.ProductionCapacityofPlasmaProteinsCapacity;MillionL/YCompanieswithyellowbarhaveappliedToyopearltotheirprocess.7.CSLBioplasma,Austr

7、aliaCommonwealthSerumLaboratory(CSL)InternationalpharmaceuticalcompanybasedinAustraliaasCSLgroupsAllprocesseswereappliedbychromatographyforCohnethanolfractionationexceptFraction1in1994Thefourthlargest