烟草疫霉侵染前后剑麻叶片转录组学研究.pdf

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1、热带作物学报2014，35(3)：576—582ChineseJournalofTrooicalCro烟草疫霉侵染前后剑麻叶片转录组学研究汪平t一．高建明2．杨峰2郑金龙刘巧莲，陈河龙，易克贤。1海南大学环境与植物保护学院．海南海口5702282裹农业部热带舞作物生物学鸶与釜遗传资源利酱用重点实验室海南海口5711013裹农昱业部热带作物有害生荔物蕤综是合治理重薹点篓实蠢验萎室海南『羊J海口571101摘要为克隆剑麻受斑马纹病病原菌烟草疫霉侵染后的应答基因．以病原菌侵染前后的剑麻H．11648叶片为材料

2、，构建剑麻转录组UnigeD．e数据库；组装得到非冗余Unine131422个，其中，500～1000bp的Unigene占总数65．39％，Unigene的数量随其长度递减。GO分类分析发现：在得到的所有Unigene中，注释到生物过程的基因最多(152835)；细胞组分其次(129197)；参与分子功能的最少(47420)KEGG代谢通路分析将剑麻转录组unigene分成128类，其中，参与生化代谢通路的Unigene最多，有9354个，占总数27．09％；参与植物一病原互作代谢通路的基因有l795

3、个，占总数5．2基因表达丰度RPKM值显示上调基因9415个．下调基因28980个．其中参与病原互作的基因占680个关键词烟草疫霉；侵染：剑麻；转录组中图分类号$563．8文献标识码ATranscriptomeofSisalLeafPretreatedwithPhytophthoranicotianaeBreda．WANGPing，GAOJianming，YANGFeng。，ZHENGJinlongLIUQiaolian，CHENHelong~，YIKexianICollegeofEnvironment

4、andPlantProtection，H~nanUniversity,Haikou，Hainnn570228,China2KeyLaboratoryofBiologyandGeneticResourcesofTropicalCrops，MinistryofAgricuhure／InstituteofTropicalBioscienceandBiotechnology,ChineseAcademyofTropicalAgriculturalSciences，Haikou，Hainan571101，Chin

5、a3KeyLaboratoryofIntegratedPestManagementonTropicalCrops，MinistryofAgrieulture／InstituteofEnvironmentandPlantProtection，ChineseAcademyofTropicalAgriculturalSciences，Haikou，Hainan571101，ChinaAbstractS／sa／leavesinfectedwithPhytophthoranicotianaeBredawereus

6、edtoconstructthetranscriptometostudytheresponsegeneofsisalinfectedbyPnicotianaeBreda．．Amongthe131．422nonredundantunigenes．65．39％werebetween500-1000bp，indicatingthatthenumberofunigeneswaslengthdependent．Theunigeneswereinvolvedinthebiologicalprocess(152835

7、)，cellularcomponent(129197)andmolecularfunction(44112)categoriesbyGOanalysis．PredictedbyKEGGpathwaymapping，thetranscriptomeofs／sa／wasdividedinto128classes．Theunigenesinvolvedinbiochemicalpathwayandplant-pathogeninteractionwas9354(27．09％)and1795(5．2％1，res

8、pectively．GeneexpressionabundanceRPKMvalueshowedthattherewere9415up—regulatedgenesand28980down—regulatedgenes，inwhichplant—pathogeninteractionrelatedgeneis680．KeywordsPhytophthoranicotianaeBreda．；Infection；Sisal；Transcript