杜仲灰斑病防治药剂的筛选-论文.pdf

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1、贵州农业科学2014，42(9)：1O6～108GuizhouAgriculturalSciences[文章编号]1001—3601(2014)09—0533—0106—03PlantProtection·SoiIandFertilizer·Microoraanism杜仲灰斑病防治药剂的筛选任明国，李荣玉。，刘红昌，龙友华，吴小毛(1．贵阳市开阳县农业局植保站，贵州开阳550025；2．贵州大学农学院，贵州贵阳550025；3．贵州省药用植物繁育与种植重点实验室，贵州贵阳550025)[摘要]为筛选出有效防治杜仲灰斑病的药剂在生产上应用，采用菌丝生

2、长速率法，研究甲基托布津、百茵清、中生菌素、多抗霉素、多菌灵和苯醚甲环唑等6种杀菌剂对杜仲灰斑病菌的室内毒力，并进行了田间药效试验。结果表明：室内毒力测定，多抗霉素、多茵灵和苯醚甲环唑对灰斑病菌有较好的抑制作用，其EC5o分别为1．67／~g／mL、0．96tzg／mL和0．18~g／mL；而甲基托布津、百茵清和中生菌素对灰斑病菌的毒力相对较弱。经田间药效试验，多抗霉素1000倍液、多茵灵2000倍液和苯醚甲环唑2500倍液对杜仲灰斑病有较好的防治效果，第2次施药10d后的防效分别可达74．36、8O．82和79．67，显著优于其他药剂处理，可作为

3、防治杜仲灰斑病的首选药剂。[关键词]杜仲；灰斑病；杀菌剂；毒力测定；药效；药剂筛选[中图分类号]$436．611．1[文献标识码]AScreeningofFungicidesControllingGrayLeafSpotDiseaseofEucommiaulmoidesRENMingguo，LIRongyu，LIUHongchang。，LONGYouhua，WUXiaomao(1．KaiyangPlantProtectionStationofAgriculturalBureau，Kaiyang，Guizhou550025；2．Agriculture

4、CollegeofGuizhouUniversity，Guiyang，Guizhou550025；3．GuizhouKeyLaboratoryofPropagationandCultivationonMedicinalPlants，Guiyang，Guizhou550025，China)Abstract：InordertoscreeneffectivefungicidescontrollinggraylearspotdiseaseofE．ulmoides，thetoxicityofsixfungicides(thiophanatemethyl，ch

5、lorotha1on订，Zhongshengmycin，polyoxins，carbendazimanddifenoconazo1e)onE．ulmoidesgrayleafspotpathogenweredeterminedinlaboratoryusingmyceliumgrowthratemethod．ThefieldtrialofthesixfungicidescontrollinggraylearspotdiseaseofE．ulmoideswasalsocarriedoutunderthefieldcondition．Results：T

6、hiophanate—methyl，chlorothalonilandzhongshengmycinhadworseantimicrobialeffectonA．tenuis．polyoxin。carbendazimanddifenoconazo1ehadbetterantimicrobialeffectonA．tenuisthanothers，withEC50of1．67og／mL，0．96g／mLand0．18￡上g／mL，respectively．Thefieldtria1resultsalsoconfirmedthattendaysafte

7、rsecondsprayedapplication，thecontrolefficiencyofpolyoxin1000times，carbendazim2000timesanddifenoconazole2500timesreached74．36，8O．82and79．67，respectively．Controleficiencyofthethreeagentswassignificantlybetterthanthatofothertreatments．Therefore，polyoxin，carbendazimanddifenoconazo

8、lecouldberecommendedasthefirst—choicefungicidestocontrolgrayl