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1、Rumenlysineescape,rumenfermentationandproductivityofearlylactationdairycowsfedfreelysineP.H.Robinsona,*,E.J.DePetersa,I.Shinzatob,H.SatobaDepartmentofAnimalScience,UniversityofCalifornia,Davis,CA,95616,USA,andAtlanticDairy&ForageInstitute,FrederictonJunction,
2、NB,E0G1T0,CanadabAjinomotoCo.,Inc.,5-8KyobashiI-Chome,Chuo-Ku,Tokyo104,Japan*Correspondingauthor.Tel:530-754-7565;Fax:530-752-0175;EM:SubmittedtoAnimalFeedScienceandTechnologyinJune2005Revisedandre-submittedinAugust2005.Revisedandre-submittedinSeptember2005.A
3、bstractTheprimaryobjectivewastoquantitateforestomachescapeoflysinefedtocowsinafreeform.Howeversinceitwasexpectedthatalargeproportionofthelysinewouldbedegradedintherumen,otherobjectivesweretodetermineiflysineimpactedruminalfermentationaswellasdetermineeffectso
4、nperformanceofthecows.FourmultiparousHolsteincows,fittedwithlargediameterrumencannulaebetween6and8weekspriortotheirprojectedcalvingdate,wereassignedina4x4Latinsquaredesignexperimentbetween2and4weekspost-partum.Allcowswerefedthesametotalmixedration(TMR)andtrea
5、tmentdifferenceswereachievedbymanuallyincorporatingL-lysineHClintoeachcow’sindividuallyweighedallocationofTMRatthetimeoffeedingtodeliver0,1,2or3gofL-lysinefromL-lysineHCl/kgofdrymatter(DM)intake,althoughactuallydeliveredlysinevalueswereabout16%higher.Asexpect
6、ed,averagerumenfreelysineconcentrationsincreasedlinearly(P=0.05)duetoincreasedfeedinglevelsoflysine.RumenpH,Nandvolatilefattyacidconcentrations,aswellasotherorganiccomponentsofrumeningesta,includingthoseofisolatedrumenbacteria,wereunaffectedbylysinefeeding.In
7、takeofDM,neutraldetergentfibreandcrudeproteinwerenotinfluencedbyincreasedfeedingofL-lysine,aswereproductionofmilkanditscomponents.Feedingincreasinglevelsoffreelysinetolactatingdairycows,inthreelevelsupto71g/d,resultedinanestimatedforestomachescapeoflysineof35
8、g/kgoflysinefed,alevelthatisonlyabout1/6ofthosereportedinpreviousstudiesbaseduponshorttermpulsedosingand/orfeedingstudies.Keywords:lysine,forestomach,escape,rumenAbbreviations:AA,aminoaci
