细胞培养PBS配方.doc

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1、Phosphate-BufferedSaline(PBS;1×,pH7.4)ReagentQuantity(g)Finalconcentration(mM)NaCl8.0137.0KCl0.20 2.7Na2HPO4（12H2O）1.14（2.874） 8.0KH2PO40.20 1.5Combineallingredientsin∼800mLofH2Oandstirtodissolve.AdjustpHto7.4andbringfinalvolumeto1L.Sterilizebyautoclaving.Themostcommoncompositionof

2、PBS(1X)SaltConcentration(mmol/L)Concentration(g/L)  NaCl  1378.0  KCl  2.70.2  Na2HPO4  101.44  KH2PO4  1.80.24Startwith800mLofdistilledwatertodissolveallsalts.AdjustthepHto7.4withHCl.Adddistilledwatertoatotalvolumeof1liter.Theresultant1xPBSshouldhaveafinalconcentrationof10mMPO43-,

3、137mMNaCl,and2.7mMKCl.