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ID:56464419
大小:15.15 MB
页数:66页
时间:2020-06-19
《分子生物学之PPT素材精编.ppt》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、生物化学与分子生物学PPT素材可根据需要选择取舍Metabolismof5-fluorouracil(5-FU)5-FUCarac,Efudex5-Fluorouridine5-FUMP5-FdUMPUridinephosphorylase5-FluorodeoxyuridineThymidinephosphorylaseOrotatephosphoribosyltransferaseThymidinekinase5-FUTP5-FdUTPRNADNA5’-nucleotidase5’-nucleotidase
2、UridinekinaseATPADPATPADPPRPPWesternBlotting1.ResolveproteinsamplesonSDS-PAGE6.IncubatetheblotwithchemiluminescentHRPsubstrateandexposetofilm2.ElectrophoreticallytransferfractionatedproteinsfromgelontoPVDFmembrane3.Blockthemembranewithneutralprotein(BSAorm
3、ilkcasein)4.Incubatethemembranewithprimaryantibodyspecifictotargetprotein5.IncubatethemembranewithHRP-labeledsecondaryantibodyspecifictoprimaryantibodyCholesterolSterolToolkitPregnenolonProgesteronAldosteronCortisolCorticosteroneAndrostendioneTestosteronEstri
4、ol17-Estradiol1,25-DihydroxycholecalciferolCholicacidCovalentmodificationofproteinsPoly(ADPribosyl)Polymerase(PARP)Poly(ADPribosyl)polymerase(PARP)NADNicotinamidePoly(ADPribosyl)polymerase(PARP)Poly(ADPribosyl)chainelongationandbranchingNicotinamideNADNADDN
5、ApolymeraseDNApolymeraseDNApolymeraseOligosacharyltransferaseN-glycosylationpathwayinERMann-P-DolicholtransferasesFlippaseDolicholPDolicholPPDolicholPPDolicholPPDolicholPPGlcNAc-PtransferaseGlcNActransferaseDolicholPDolicholPPDolicholPPGDP-Mannosyltransfera
6、sesGlc-P-DolicholtransferasesDolicholPDolichol-pyrophofphataseCytosolER40S60SUTPCTPTMPPyrimidineNucleotideInterconversionsUMPATPADPH2OUDPNucleosidemonophosphatekinaseATPADPNucleosidediphosphatekinaseATPADPCTPsynthaseRibonucleotidereductasedeoxyUDPNucleoside
7、diphosphatephosphohydrolasedeoxyUMPThymidylatesynthaseN5,N10-MethyleneTHFDHFTTPNucleosidemonophosphatekinaseNucleosidediphosphatekinaseMyo-Inositol-3-phosphateIns(3)P1BiosynthesisandmetabolismofphosphoinositidesGlucose6-pgosphateIns(3)P1synthaseIns(3)P1pho
8、sphataseMyo-InositolInsPtdInssynthasePtdInsMyo-inositolkinasePtdIns-4-PPtdIns4-kinasePtdIns5-kinasePtdIns-4,5-P2PI3KPtdIns-3,4,5-P3PLCPLCPI3KPtdIns
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