英文文献报告.ppt

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1、ReadingReportTheeffectofmixotrophyonmicroalgalgrowth,lipidcontent,andexpressionlevelsofthepathwaygenesinChlorellasorokinianaIF=3.425主要内容本文围绕生物能源微藻的培养进行研究。对目的微藻进行培养优化，对微藻的光合自养、异养和混合培养对其生长、脂质影响进行比较和分析，认为混合培养即可以大量促进微藻的生物量的累积并提高微藻的脂含量，而且代价较异养要低，可以作为微藻大规模培养的可选择手段。且C.sorokinianaCCTC

2、CM209220要优于另外两种微藻。本文通过实时PCR，对（accD、acc1、rcbL）基因在C.sorokinianaCCTCCM209220中的表达量进行测试，并将表达差异和脂质含量进行比较，选出了和脂质含量密切相关的基因作为将来基因改造的候选基因。认为在非异养条件下，accD基因的表达水平可作为评估脂质含量的检验手段之一。ContentsIntroduction1.Results2.Discussion3.Benefits4.IntroductionThesearchofanimprovedrawmaterialisoneofthemajo

3、robjectivesinbiodieselresearch.Microalgaehavemanyadvantagesfortheproductionofbiodieselcomparedtootheroilcrops.IntroductionThecultivationofmicrolagaedoesnotrequirelargeareasofarable.AdvantagesMicroalgaecangrowrapidly,andmanymicroalgaespeciesarerichinoils.IntroductionHeterotroph

4、yMixotrophyPhotoautotrophyWithoutorganiccarbonsources.SlowgrowthLowestcostRelayonorganiccarbonsourcesFastgrowthHighestcostcomplementphotoautotrophywithorganicmaterialFastgrowthRelativelycheapMixotrophicMixotrophiccultivationcancomplementphotoautotrophywithorganicmaterial,thuss

5、horteningthegrowthcycle,increasingbiomassyield,anddecreasingthecostofheterotrophicmicroalgaecultivation.IntroductionAcetyl-CoAcarboxylase(ACCase)Inthebranchpoint,acetyl-CoAcarboxylase(ACCase)cancatalyzeacetyl-CoAtobeconvertedtomalonyl-CoAandtoenterthepathwayoflipidbiosynthesis

6、.Thisreactionisacriticalstepinfattyacidbiosynthesis,soACCaseisconsideredakeyenzymeforlipidaccumulation.IntroductionaccDheteromericacetyl-CoAcarboxylasebetasubunit.acc1homomericacetyl-CoAcarboxylasercbLribulose1,5-bisphosphatecarboxylase/oxygenaselargesubunitIntroductionIntrodu

7、ctionThispaperanalyzedtheexpressionofthreedifferentgenes(accD,acc1,andrbcL)inthisstrainunderdifferentmixotrophicconditionsbyreal-timePCR.Inordertostudygeneregulationrelatedtolipidsynthesis,andexaminedtherelativeexpressionofthreegeneswithrespecttogrowthandoilaccumulation,whicho

8、ffersusefulinsightsintosubsequentstepstoimprovethisparticular