分子生物学样卷答案.doc

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1、华中科技大学生命学院2007-2008学年度第二学期考试试卷《分子生物学》考试试题B卷闭卷考试时间：150分钟专业班级姓名学号分数PARTI:DESCRIPTION(3pointseach)1.microsatelliteDNA：AclassofrepetitiveDNAthatiscomposedofveryshort,tandemlyrepeatedsequences.ThemostcommonmicrosatelliteDNArepeatsarecomposedofrepeatsoftwoorthreenucleotides(e.g

2、.,CACACACA)2.slidingDNAclamp:Amultisubunitproteinthatformsaring-shapedstructurethatcanencircledouble-strandedDNA.WhenencirclingtheDNA,thisproteincanassociatewithotherenzymes(e.g.,DNApolymerases)andinhibittheirdissociationfromtheDNAtherebyincreasingtheirprocessivity3.snRNP

3、:Asubunitofthespliceosome.EachsnRNPcontainsbothaproteinandanRNAcomponent,thelatterbeingcalledasmallnuclearRNA.4.transitions:Asimpletypeofmutationinvolvingapyrimidine-to-pyrimidinesubstitutionorapurine-to-purinesubstitution5.branchmigration:TheprocessbywhichaHollidayjuncti

4、onmovestoanewlocationwithinafour-strandedDNArecombinationintermediate.6.Shine-Dalgarnosequence：SD序列，Aconservedsequence8-13ntupstreamofthefirstcodontobetranslated.ThissequencewasdiscoveredbyShineandDalgarno.Thesequenceispurine-richandcontainsallorpartof5’-AGGAGGU-3’whichca

5、nbase-pairwiththe3’-endofthe16SrRNA7.Frameshiftmutation:Insertionsordeletionsofoneorasmallnumberofbasepairsthatalterthereadingframe.8.DNAcloning：TheabilitytoconstructrecombinantDNAmoleculesandmaintainthemincellsiscalledDNAcloning.9.Restrictionendonucleases：Restrictionendo

6、nucleases(RE)arethenucleasesthatcleaveDNAatparticularsitesbytherecognitionofspecificsequences.10.aminoacyltRNAsynthetase：AnenzymethatcatalyzestheattachmentofanaminoacidtoacognatetRNAthroughtwosteps:adenylylationofaminoacidandtRNAcharging.PARTII:SINGLECHOICES(2pointseach)1

7、.AnonsensemutationisachangeintheDNAsequencethatresultsin:（3）1)asmalldeletionorinsertion.2)anaminoacidchangeintheproteinencodedbythegene.3)aprematurestopcodon.4)noneoftheabove.2.Bidirectionalandsemi-conservativearetwotermsthatreferto:（3）1)transcription.2)translation.3)repl

8、ication.4)alloftheabove.5)noneoftheabove.3.RNApolymeraseIistheeukaryoticenzymeresponsiblefor:（1）