柿叶提取物对高血糖小鼠降血糖作用及其机制探讨.pdf

《柿叶提取物对高血糖小鼠降血糖作用及其机制探讨.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、L0czr0N工mJ】z0n工一zm∽m王m0一n广cz一

2、，FBG)值和血清果糖(fructosamine，FRU)值，评价柿叶提取物的降血糖效果；从糖消化吸收、肝糖原合成、肌肉己糖激酶(hexokinase，HK)活性、血清TC及TG含量、肝脏SOD活性和MDA含量及胰岛组织形态等方面，初步探讨柿叶提取物降血糖作用机制。[结果】与模型组比较，柿叶提取物高、低剂量均能显著降低小鼠FBG值，但对FRU无明显影响；柿叶提取物高剂量组能增加肝糖原的合成、提高肌肉HK的活性、降低血清TC、TG、肝组织MDA的含量及增加肝组织SOD的活性；但对胰腺损伤无明显修复作用。【结论】柿叶提取物可降低高血糖小鼠空

3、腹血糖值，其作用可能与增加肝糖原的合成、提高肌肉HK的活性、降低血清TC、TG、肝组织MDA的含量及增加肝组织SOD的活性等有关。关键词：柿叶提取物；降血糖；四氧嘧啶；空腹血糖；作用机制中图分类号：R331文献标识码：A文章编号：10o5-55o9(2013)11—1330-06HypoglyeemicEfectandMechanismsofPersimmonLeafExtractonHyperglycemicMiceZhangLing，LiuQi，MaoLei，etalCollegeofPharmaceuticalSciences，Z

4、hejiangChineseMedicalUniversity，Hangzhou《310o53)Abstract：[O~ective]ToinvestigatethehypoglycemicefectanditspossiblemechanismsofpersimmonleafextractonhyperglycemiamiceinducedbyALx．[Method】Usingwaterextractionandalcoholprecipitationprocessforpreparingpersimmonleafextractand

5、tailveini~ectionALXtoestabhshhy—perglycemicmicemode1．Bydeterminatingmicef~fingbloodglucose(FBG)valueandserumfuctosamine(FRU)valuetoevaluatethehypoglycemicefectofpersimmonleafextract．Todiscussthehypoglycemicmechanismsbytestingthedigestionandabsorptionofsugar，hverglycogens

6、ynthesis，muscleHex—okinase(HK)activity，semlnTCandTGconten~，SODactivityandMDAcontentsintheliverandpancreasisletorganizationform，etc．【Result]Corn—paredwiththemodelgroup，persimmonleafextracthigh，lowdosegroupscansignificantlyreducethemiceFBGvalue，buthavenoobviousefectonFRUva

7、lue．Persimmonleafextracthighdosegroupcanincreasefiverglycogensynthesis，improvemuscleHKactivity，reduceserumTCandTGcon—tents，increaseSODactivityandMDAcontentinlivertissue，buthasnoobviousrepairofpancreaucinjury．[Conclusion]Pemimmonleafextraccanre—ducethehyperglycemiamiceFBG

8、value，whichmayberelatedtOtheincreasingofliverglycogensynthesis，improvmentofmuscleHKactivity，reductionof