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时间:2020-06-19
《低温胁迫对4种幼苗的叶绿素荧光特性的影响.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、第34卷第2期中南林业科技大学学报、,01.34No.22014年2月JournalofCentralSouthUniversityofForestry&TechnologyFeb.2014低温胁迫对4种幼苗的叶绿素荧光特性的影响卢广超,许建新,薛立,张柔,吴彩琼,邵怡若(1.华南农业大学林学院,广东广州510642;2.深圳市铁汉生态环境股份有限公司,广东深圳518040)摘要:采用人工气候箱的方法,测定了低温胁迫下葛藤Puerarialobata、假连翘Durantarepens、老鸭嘴Thunbergiae
2、recta和台湾相思Acaciaconfuse幼苗的荧光参数,以便为筛选抗寒种类提供依据。结果表明,随着低温胁迫时间的延长,4种幼苗的最大荧光()、表观电子传递速率(ETR)、PSII实际光量子效率Y(II)逐渐下降,非光化学淬灭(NPQ)先上升后下降,PSII调节性能量耗散Y(NPQ)和PSII非调节性能量耗散Y(NO)逐渐升高。经过24h自然恢复,4种幼苗的均恢复到对照水平,ETR有所回升,Y(NPQ)恢复到显著高于对照水平;葛藤、老鸭嘴和台湾相思的NPQ保持稳定,假连翘的NPQ有所下降;假连翘和台湾相思的Y
3、(II)恢复到对照水平,葛藤、假连翘和台湾相思幼苗的Y(N0)恢复到对照水平。对荧光参数进行主成分分析表明,干旱胁迫下4种幼苗的抗寒顺序为台湾相思>假连翘>葛藤>老鸭嘴。关键词:低温胁迫;幼苗;叶绿素荧光参数;主成分分析中图分类号:$718.43文献标志码:A文章编号:1673—923X(2014)02—0044-06Effectsoflowtemperaturestressonchlorophyllfluorescencecharacteristicsoffourtypesoftreespeciesseedli
4、ngsLUGuang—chao,XUJian—xin一,XUELi,ZHANGRou,WUChai—qiong,SHAOYi—ruof1.CollegeofForestry,SouthChinaAgriculturalUniversity,Guangzhou510642,Guangdong,China;2.ShenzhenTechandEcology,EnvironmentCO.LTD.,Shenzhen518040,Guangdong,China)Abstract:Inordertoprovidethescie
5、ntificbasisforselectingcoldresistanceseedlingstype,thechlorophyllfluorescencecharacteristicsofPuerarialobata.Durantarepens.ThunbergiaerectaandAcaciaconfusewerestudiedunderlowtemperaturestress.Theresultsshowedthatwithincreasingtimeoflowtemperaturestress,thechl
6、orophyllfluorescencecharacteristicsofmaximalfluorescence(Fm),electrontransportrate(ETR)andactivequantumeficiencyofPSII(Y(II))offourseedlingstypesdecreasedgradually,whereastheirnon—photochemicalquenching(NPQ)firstroseandthendeclined,andthequantumyieldofregulat
7、edenergydissipation(Y(NPQ))andquantumyieldofnon—regulatedenergydissipation(YN0)increasedgradually;After24hours’naturalrecovery,therecoveredtothecontrollevel,ETRrose,whereastheY(NPQ)wassignificantlyhigherthancontrollevelforthefourseedlingstypes;ThevalueofNPQof
8、Plobata,erectaandA.confuseremainedstable,whereasthatofD.repensdeclined;TheY(II)ofD.repensandA.confuse,andtheYfNO)ofPlobata,D.repensandA.confuserecoveredtothecontrollevel;Thechlorophyllflu
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