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时间:2020-06-19
《旱盐交叉胁迫对燕麦幼苗叶片生理特性的影响.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、第32卷第3期干旱地区农业研究V01.32No.32014年5月dturalResearchintheAridAreasMav20l14旱盐交叉胁迫对燕麦幼苗叶片生理特性的影响刘建新,王金成,王瑞娟,贾海燕(陇东学院生命科学与技术学院/甘肃省高校陇东生物资源保护与利用省级重点实验室,甘肃庆阳745000)摘要:以‘定莜6号’燕麦幼苗为试材,分析了旱盐交叉胁迫下叶片02-和H202产生、膜脂过氧化及抗氧化系统的变化。结果表明,随着干旱和盐胁迫程度的提高,叶片活性氧代谢失调,细胞膜受到严重伤害,表现为SOD、POD和APX活性先升高后降低,
2、AsA含量下降,CAT活性及GSH和丙二醛(MDA)含量升高,02和02产生增加。0.3%的土壤含盐量可显著提高轻度(土壤含水量占田问持水量65%)和中度(土壤含水量占田间持水量50%)干旱胁迫下燕麦幼苗叶片SOD、POD和APX的活性,降低02产生速率及H202和MDA含量;0.6%的土壤含盐量对轻度干旱胁迫燕麦幼苗叶片的02产生速率及H202和MDA含量无明显影响,却使中度干旱胁迫下的02产生速率及H202和MDA含量显著升高;在重度干旱(土壤含水量占田间持水量35%)胁迫下,随着土壤含盐量的增加,燕麦叶片SOD、CAT、POD、A
3、PX活性和AsA、GSH含量显著下降,o2产生速率及H,0,和MDA含量明显增加。表明适量的土壤含盐量可通过提高抗氧化系统的活性缓解轻、中度干旱胁迫对燕麦幼苗的伤害,而过量的盐分则加重干旱损伤。关键词:燕麦;旱盐交叉胁迫;抗氧化系统;膜脂过氧化中图分类号:$512.6;Q945.78文献标志码:A文章编号:1000—7601(2014)03.0024.05EfectsofdroughtandsalinitycombinedstressonactivitiesofphysiologicalcharacteristicsinAvenanud
4、aseedlingleavesLIUJian—xin,WANGJin—cheng,WANGRui—juan,JIAHai—yan(University胁,lcKeyLaboratoryforProtectionandUtilizationofLongdongBio-resourcesinC.ansuProvince;CollegeofLifeScienceandTechnology,LongdongUniversity,Qingyang,Gansu745000,PRChina)Abstract:Thepotcultureexperime
5、ntwasconductedtostudytheresponsetodroughtandsalinitycombinedstressofreactiveoxygenspecies(ROS),including02andH202,aswellasmalondialdehyde(MDA)contentandantioxidativesysteminleavesofnakedoat(AvenanudaL.‘DingyouNo’6)seedlings.I1hemsuhsshowedthatthecellmembranewasseriouslyd
6、amagedduetothedisturbedROSmetabolism,activitiesofcellantioxidantenzymesandantioxidantcon—tent.Catalase(CAT)activityandglutathione(GSH)contentincreased,aseorbicacid(AsA)contentdecreased.Super—oxidedismutase(SOD),peroxidase(POD),andascorbateperoxidase(APX)increasedtothepea
7、kandthendecreased.Furthermore,02generationrateandcontentsofH202andMDAsignificantlyincreasedinleavesofnakedoatseedlings.Comparedwiththecontrolsoil.salinityof0.3%significantlyenhancedtheactivitiesofSOD.PODandAPXinleavesundertheconditionsoflowdroughtstress(65%soilfieldcapac
8、ity)andmediumstress(50%soilfieldcapacity).Inaddition.soilsalinityat0.6%showednosignificantinflHenceonRO
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