常用缓冲液配方及缓冲范围.pdf

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1、常见缓冲液的缓冲范围缓冲液名称及常用浓度缓冲pH范围主要物质分子量MES(2吗啉代乙磺酸)5.5-6.7195.2Bis-Tris5.8-7.2209.2HEPES6.8-8.2238.3PIPES6.1-7.5302.4MOPS6.5-7.9209.3Tricine7.4-8.8179.2TEA(三乙醇胺)7.4-8.3149.2甘氨酸-盐酸缓冲液（0.05mol/L）2.2-5.0甘氨酸Mr=75.07邻苯二甲酸-盐酸缓冲液（0.05mol/L）2.2-3.8邻苯二甲酸氢钾Mr=204.23磷酸氢二钠-柠檬酸缓冲液

2、2.2-8.0磷酸氢二钠Mr=141.98柠檬酸-氢氧化钠-盐酸缓冲液2.2-6.5柠檬酸Mr=192.06柠檬酸Mr=192.06柠檬酸-柠檬酸钠缓冲液（0.1mol/L）3.0-6.6柠檬酸钠Mr=257.96乙酸钠Mr=81.76乙酸-乙酸钠缓冲液（0.2mol/L）3.6-5.8乙酸Mr=60.05邻苯二甲酸氢钾-氢氧化钠缓冲液4.1-5.9氢钾Mr=204.23磷酸氢二钠-磷酸二氢钠缓冲液（0.2mol/L）5.8-8.0磷酸氢二钠-磷酸二氢钾缓冲液（1/15molL）4.92-8.18磷酸二氢钾-氢氧化钠缓

3、冲液（0.05mol/L）5.8-8.0巴比妥钠-盐酸缓冲液(18℃)6.8-9.6巴比妥钠Mr=206.18三羟甲基氨基甲烷（Tris）Tris-盐酸缓冲液(0.05mol/L25℃)7.10-9.00Mr=121.14甘氨酸-氢氧化钠缓冲液(0.05mol/L)8.6-10.6甘氨酸Mr=75.07硼砂-氢氧化钠缓冲液(0.05mol/L)9.3-10.1硼砂Na2B4O7·10H2OMr=381.43碳酸钠Mr=286.2碳酸钠-碳酸氢钠缓冲液(0.1mol/L)9.16-10.83碳酸氢钠Mr=84.0碳酸钠-

4、氢氧化钠缓冲液(0.025mol/L)9.6-11.0磷酸氢二钠-氢氧化钠缓冲液10.9-12.0氯化钾-盐酸缓冲液（0.2mol/L）1.0-2.2氯化钾Mr=74.55氯化钾-氢氧化钠缓冲液（0.2mol/L）12.0-13.0氯化钾Mr=74.55参考下图：常见缓冲液的缓冲范围常用缓冲液的配制方法1.甘氨酸-盐酸缓冲液（0.05M）Xml0.2M甘氨酸+Yml0.2M盐酸再加水稀释至200mlpHX/mlY/mlpHX/mlY/ml2.25044.03.05011.42.45032.43.2508.22.6502

5、4.23.4506.42.85016.83.6505.0甘氨酸分子量＝75.070.2M甘氨酸溶液含15.01g/L2.邻苯二甲酸-盐酸缓冲液（0.05M）Xml0.2M邻苯二甲酸氢钾+Yml0.2M盐酸再加水稀释至200mlpHXYpHXY2.254.6703.251.4702.453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.052.032邻苯二甲酸氢钾分子量＝2.4.230.2M邻苯二甲酸氢钾溶液含40.85g/L3.磷酸氢二钠-柠檬酸缓冲液pH0.2M0

6、.1M柠檬酸pH0.2M0.1M柠檬Na2HPO4/ml/mlNa2HPO4/ml酸/ml2.20.4019.65.210.729.282.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.812.097.913.04.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.

7、07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.708.019.450.55Na2HPO4分子量＝141.980.2M溶液含28.40g/LNa2HPO4·2H2O分子量＝178.050.2M溶液含35.61g/LC6H8O7·H2O分子量＝210.140.1M溶液含21.01g/L4.柠檬酸-氢氧化钠-盐酸缓冲液

8、pH钠离子浓度柠檬酸氢氧化钠浓盐酸终体积/L/MC6H8O7·H2O/gNaOH/gHCl/ml2.20.2021084160103.10.2021083116103.30.2021083106104.30.202108345105.30.3524514468105.80.45285186105106.50.38266156126