插壤温度对四倍体刺槐硬枝插条生根过程中生理生化的影响.pdf

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1、植物生理学报PlantPhysiologyJournal2012，48(7)：699-704699插壤温度对四倍体刺槐硬枝插条生根过程中生理生化的影响王小玲，赵忠’，高柱。江西省科学院生物资源研究所，南~330029；西北农林科技大学，西部环境与生态教育部重点实验室，陕西杨凌712100摘要：取四倍体刺槐3年生采穗圃中1年生硬枝插条为材料，研究扦插过程中插壤温度对插穗内源激素含量、酶活性和营养物质含量的影响，揭示四倍体刺槐扦插生根机制。结果表明：四倍体刺槐硬枝扦插过程中，插壤经加热处理，插穗内源IAA含量降低，ABA和ZR含量升高；根原基诱导期插穗内源IAA含量的变化趋势与未加热插穗相反，

2、ABA和ZR含量的变化趋势与未加热插穗相同。插壤经加热处理，插穗IAAO、PPO和POD活性的变化趋势与未加热插穗相同，两处理间插穗IAAO、PPO和POD活性差异不显著。插壤经加热处理，可显著提高插穗可溶性蛋白和可溶性糖含量，而对淀粉和植物总氮含量的影响差异不显著。因此，插壤加热处理，主要是通过降低四倍体刺槐硬枝插穗内源IAA含量，提高插穗可溶性蛋白和可溶性糖含量来促进插条不定根发生。关键词：四倍体刺槐；插壤温度；内源激素；酶活性；营养物质EffectsofPlugSoilTemperatureonPhysiologicalandBiochemicalinHard—woodCutting

3、sRootingofTetraploidRobiniapseudoacaciaWANGXiao．Ling，ZHAOZhong’，GAOZhu：InstituteofBiologicalResources．JiangxiAcademyofSciences．Nanchang330029,China,"。KeyLaboratoryofEnvironmentandEcologyinWesternChinaofMinistryofEducation，NorthwestA＆FUniversity,Yangling,Shaanxi712100,ChinaAbstract：Annualhardwoodcu

4、ttingsin3一year．oldcuttingorchardoftetraploidRobiniapseudoacaciaweretakenasmaterialsinthisexperiment．Effectsofplugsoiltemperatureonendogenoushormonescontents，oxi—daseandantioxidantenzymeactivity,nutrientcontentsofhardwoodcuttingswerecarriedouttoelucidatetherootingmechanismoftetraploidR．pseudoacacia

5、．Theresultshowed：endogenousIAAcontentofhardwoodcuttingsdecreasedwithplugsoilheatingduringrootingprocess．andincreasedinABAandZRcontents．Dur-ingtherootprimordialinitiationphase．contrast仃endsinIAAcontentandsimilartrendsinABAandZRcon—tentstothoseobservedintheheating．treatedandwithoutheating—treatedcut

6、tings．InadditionthetrendsofIAAO．PPOandPODactivitywiththeheating—treatedweresimilartothewithoutheating．treatedcuttings．TherewasnosignificantdiferenceinIAAO．PPOandPODactivitybetweenthetwotreatmentscuttings．Last-ly,solubleproteinandsugarcontentswiththeheating-·treatedweresignificantlyhigherthanthosew

7、ithoutheat-ing．treatedcuttings．However,therewerenosignificantdifierencesinstarchandtotalnitrogencontentsbe—tweentheheating—treatedandwithoutheating—treatedcuttings．Asaresult，theefectofplugsoilwithheating—treatedo