缓冲液配制方法.doc

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1、一．常用缓冲溶液的配制方法1．甘氨酸—盐酸缓冲液（0.05mol/L）xml0.2mol/L甘氨酸＋yml0.2mol/LHCl，再加水稀释至200mlpHx/mly/mlpHx/mly/ml2.25044.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸Mr=75.07，0.2mol/L甘氨酸溶液含15.01g/L2．甘氨酸—氢氧化钠缓冲液（0.05mol/L）xml0.2mol/L甘氨酸＋yml0.2mol/LNaOH，加水稀释至200mlpHx/mly/mlpHx/mly/ml8.6504.09.6502

2、2.48.8506.09.85027.29.0508.810.05032.09.25012.010.45038.69.45016.810.65045.5甘氨酸Mr=75.07，0.2mol/L甘氨酸溶液为15.01g/L3．邻苯二甲酸—盐酸缓冲液（0.05mol/L，20℃）xml0.2mol/L邻苯二甲酸氢钾＋yml0.2mol/LHCl，再加水稀释到20mlpHx/mly/mlpHx/mly/ml2.254.6703.251.4702.453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.052.032   邻苯二甲酸氢钾Mr=20

3、4.23，0.2mol/L邻苯二甲酸氢钾溶液含40.85g/L4．柠檬酸—氢氧化钠—盐酸缓冲液10pHNa＋浓度/mol/L柠檬酸/gC6H8O7·H2O氢氧化钠/gNaOH(97%)盐酸/mlHCl(浓)最终体积/L*2.20.2021084160103.10.2021083116103.30.2021083106104.30.202108345105.30.3524514468105.80.45285186105106.50.3826615612610使用时可以每升中加入1g酚，若最后pH值有变化，再用少量50%氢氧化钠溶液或浓盐酸调节，置冰箱保存。5．磷酸氢二钠—柠檬酸缓冲液pH0.

4、2ol/LNa2HPO4/ml0.1mol/L柠檬酸/mlpH0.2mol/LNa2HPO4/ml0.1mol/L柠檬酸/ml2.20.4019.605.210.729.282.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.812.097.913.04.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.4

5、73.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.708.019.450.5510Na2HPO4Mr=141.98，0.2mol/L溶液为28.40g/L，Na2HPO4·2H2OMr=178.05，0.2mol/L溶液为35.61g/LC6H8O7·H2OMr=210.14，0.1mol/L溶液为21.01g/L6．柠檬酸—柠檬酸钠缓冲液（0.1mol/L）pH0.1mol/L柠檬酸/ml0.1mol/L柠檬酸钠/ml

6、pH0.1mol/L柠檬酸/ml0.1mol/L柠檬酸钠/ml3.018.61.45.08.211.83.217.22.85.27.312.73.416.04.05.46.413.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48.66.42.018.04.610.39.76.61.418.64.89.210.8   柠檬酸C6H8O7·H2O，Mr=210.14，0.1mol/L溶液为21.01g/L柠檬酸钠Na3C6H5O7·2H2O，Mr=294.12，0.

7、1mol/L溶液为29.41g/L7．乙酸—乙酸钠缓冲液（0.2mol/L，18℃）pH0.2mol/LNaAc/ml0.2mol/LHAc/mlpH0.2mol/LNaAc/ml0.2mol/LHAc/ml3.60.759.254.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.657.355.48.601.404.43.706.305.69.100.