Cu2＋在黑水虻体内迁移及对其发育影响-论文.pdf

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1、第37卷第3期2014年6月遵义医学院学报Vo1．37No．3JournalofZunyiMedicalUniversityJun．2014Cu2+在黑水虻体内迁移及对其发育影响夏嫱，朱伟，廖业，喻国辉，方旭，杜联峰(1．遵义医学院珠海校区，广东珠海519041；2．珠海市农业科学研究中心，广东珠海519075)[摘要]目的探讨饲料中cu在黑水虻体内迁移规律及对其发育影响。方法以添加不同浓度cu“的人_T饲料饲喂黑水虻幼虫，利用等离子体原子发射光谱仪测定各发育阶段虫体的cu。含量；利用昆虫毒理学方法对其发育影响进行研究。结果黑水虻各发育阶

2、段虫体cu含量均随处理浓度增加而增加，1200mg·kg处理浓度时cu“含量最高；Cu可随黑水虻的发育而迁移，高浓度胁迫下迁移规律为：蛹>6龄幼虫>成虫。cu“胁迫对黑水虻幼虫存活率、化蛹率、成虫寿命及雌雄性比均无显著影响，但高浓度处理可显著降低蛹的羽化率并延长幼虫及蛹发育所需时间。结论cu可在黑水虻体内进行迁移，高浓度胁迫下迁移规律为：蛹>6龄幼虫>成虫；仅一个世代cu“胁迫对黑水虻幼虫存活率、化蛹率、成虫寿命及雌雄性比影响较小，主要降低蛹的羽化率及延长幼虫及蛹发育所需时间。[关键词]铜；胁迫；黑水虻；累积；发育[中图法分类号]Q969

3、．97[文献标志码]A[文章编号]1000—2715(2014)03-0300-04MigrationofCuinblacksoldierfliesanditseffectsonthedevelopmentXiaQiang，ZhuWei，LiaoYe，YuGuohui，FangXu，DuLianfeng(1．ZhuhaiCampusofZunyiMedicalUniversity，ZhuhaiGuangdong519041，China；2．ZhuhaiAgricultureRe—searchCenter，ZhuhaiGuangdong51

4、9075，China)[Abstract]ObjectiveToinvestigatethemigrationdisciplinesofCuinblacksoldierfliesanditseffectsonthedevelopment．MethodsBlacksoldierflylarvaewerefedwithdifferentconcentrationsofCuhCu¨coflcentra．．tionsininsectofeachdevelopmentalstagesweremeasuredbyplasmaatomicemissio

5、nspectrometer．EffectsofCuonthedevelopmentwereassessedbyinsecttoxicologymethod．ResultsCuconcentrationsininsectofeachdevelopmentalstageswereincreasedwiththeelevatedconcentrationsofCutreatments，inwhichCu(1200mg．kg)treatmentleadtothehighestCu¨concentrationsininsect．Cumigrated

6、withthedevelopmentofblacksoldierfliesandthemigrationdisciplineafterhighCuconcentrationstreatmentwasinpupation>sixin—starlarva>adult．Therewasnosignificantdifferenceoflarvasurvivalrate，Pupationrate，adultlongevityandsexratiointhestressofCuonblacksoldierflies，whereashighCucon

7、centrationtreatmentsignificantlyre—ducedtheeclosionrateandprolongedthedevelopmentoflarvaandpupation．ConclusionCumigratesinblacksoldierfliesanditsmigrationdisciplinesafterhighCuconcentrationstreatmentwasinpupation>sixinstarlar—va>adult．OnlyonegenerationofCu“stresshasnosign

8、ificantdifferencesinlarvasurvivalratepupatingrate，，adultlongevityandsexratioandmainlyreduceseclo