不同负荷的游泳运动对大鼠认知能力及海马神经疲劳的影响-论文.pdf

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1、中国实验诊断学2014年3月第18卷第3期一377一文章编号：1007—4287(2014)03—0377—03不同负荷的游泳运动对大鼠认知能力及海马神经疲劳的影响刘忠民，高拮，牛娜，董炜达(吉林大学体育学院，吉林长春130012)摘要：目的探讨不同负荷游泳运动对大鼠学习记忆的认知能力及海马神经型一氧化氮合酶(n-NOS)表达与疲劳机制。方法将4O只体重为350—400g的Wistar雄性大鼠分为对照组和每天进行15mins、30mins、60rains的小中大负荷游泳运动的实验组；实验持续8周后采

2、用“Y迷宫”进行学习记忆能力测试；利用S-P免疫组化染色法检测海马神经n-NOS的表达水平。结果学习记忆的达标次数和错误次数，中等负荷组显著低于对照组和大负荷组(P<0．05和P％0．01)；与对照组相比中、大负荷组海马神经n—NOS阳性表达均显著提高(P<0．01)；与小负荷组比较，中负荷组和大负荷组均有显著性差异(P

3、高；大运动负荷组学习记忆能力下降而n—NOS表达水平提高，其相互作用机制有待于深入研究。关键词：游泳运动；学习记忆；海马神经；一氧化氮合酶中图分类号：R338．3文献标识码：ATheInfluenceofVariousSwimmingLoadsonRats’CognitiveCapabilityandHippocampalNeuronFatigueLIUZhong—min，GAOZhe，NIUNa，eta1．(PhysicalFpducationCollege，JilinUniversity，Cha

4、ngchun130012，China)Abstract：ObjectiveThepurposeistoexplorethefatiguemechanismofrats’cognitiveabilitytolearnandmemo—rizeandtheexpressionlevelsofhippocampalneuronn—NOSundervariousswimmingloads．Methods40Wistarmaleratsweighing350—-400garedividedintoacontro

5、lgroupand3experimentalgroupsthatswimundervariousloadsevery—-day，for15rain，30min，and60minrespectively．After8weeks，theratsreceiveabilitytestsoflearningandmemorizingthoughaY—mazeandthelevelsofhippocampalneuronn-NOSexpressionaremeasured，bymeansofummunitygr

6、oupdyeing．Resultsinthetimesofpassandfailure，thegroupundermoderateloadissignificantlylowerthanthecontrolgroupandthegroupunderlargeload(P<0．05，P<0．01)；comparedwiththecontrolgroup，positiveexpressionofhippocampalneuronn-NOSlevelsofthegroupsundermoderateand

7、largeloadsincreasesignificantly(P％0．01)；thereisasignificantdifferencebetweenthegroupundersmallloadandgroupsundermoderateandlargeloads(P％0．05，P<0．01)；thereisalsoasignificantdifferencebetweenthegroupundermoderateloadandtheoileunderlargeload(PdO．O1)．Concl

8、usionswimmingofmoderateloadcaneffectivelyimproverats’abilitytolearnandmemorize；thelevelsofhippocampalneuronn—NOSexpressionincreasesignificantlyalongwiththeincreaseoftheloads：theabilitytolearnandmemorizeofthegroupunderlargeloaddecreasesw